Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
Identifieur interne : 002F40 ( PubMed/Curation ); précédent : 002F39; suivant : 002F41Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
Auteurs : Fanya Zeng [République populaire de Chine] ; Ken Yan Ching Chow ; Chung Chau Hon ; Ka Man Law ; Chi Wai Yip ; Kwok Hung Chan ; Joseph S Malik Peiris ; Frederick Chi Ching LeungSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Anticorps (immunologie), Expression des gènes, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Immunisation, Immunité cellulaire, Immunoglobuline G (immunologie), Plasmides (génétique), Plasmides (immunologie), Production d'anticorps (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Sous-unités de protéines (immunologie), Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- génétique : Glycoprotéines membranaires, Plasmides, Protéines de l'enveloppe virale, Protéines recombinantes, Vaccins à ADN, Virus du SRAS.
- immunologie : Anticorps, Glycoprotéines membranaires, Immunoglobuline G, Plasmides, Protéines de l'enveloppe virale, Protéines recombinantes, Sous-unités de protéines, Vaccins à ADN, Virus du SRAS.
- Animaux, Expression des gènes, Glycoprotéine de spicule des coronavirus, Immunisation, Immunité cellulaire, Production d'anticorps, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies (immunology), Antibody Formation (drug effects), Gene Expression, Immunity, Cellular, Immunization, Immunoglobulin G (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Plasmids (genetics), Plasmids (immunology), Protein Subunits (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Vaccines, DNA (genetics), Vaccines, DNA (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Immunoglobulin G, Membrane Glycoproteins, Protein Subunits, Recombinant Proteins, Vaccines, DNA, Viral Envelope Proteins.
- drug effects : Antibody Formation.
- genetics : Plasmids, SARS Virus.
- immunology : Plasmids, SARS Virus.
- Animals, Gene Expression, Immunity, Cellular, Immunization, Mice, Mice, Inbred BALB C, Spike Glycoprotein, Coronavirus.
Abstract
The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.
DOI: 10.1016/j.bbrc.2004.01.166
PubMed: 14985131
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virale</term><term>Protéines recombinantes</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps</term><term>Glycoprotéines membranaires</term><term>Immunoglobuline G</term><term>Plasmides</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Sous-unités de protéines</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plasmids</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Gene Expression</term><term>Immunity, Cellular</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des 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We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14985131</PMID><DateCompleted><Year>2004</Year><Month>04</Month><Day>20</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-291X</ISSN><JournalIssue CitedMedium="Print"><Volume>315</Volume><Issue>4</Issue><PubDate><Year>2004</Year><Month>Mar</Month><Day>19</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem. Biophys. Res. Commun.</ISOAbbreviation></Journal><ArticleTitle>Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.</ArticleTitle><Pagination><MedlinePgn>1134-9</MedlinePgn></Pagination><Abstract><AbstractText>The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Fanya</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Zoology, The University of Hong Kong, Pokfulam Rd, Hong Kong, SAR, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chow</LastName><ForeName>Ken Yan Ching</ForeName><Initials>KY</Initials></Author><Author ValidYN="Y"><LastName>Hon</LastName><ForeName>Chung Chau</ForeName><Initials>CC</Initials></Author><Author ValidYN="Y"><LastName>Law</LastName><ForeName>Ka Man</ForeName><Initials>KM</Initials></Author><Author ValidYN="Y"><LastName>Yip</LastName><ForeName>Chi Wai</ForeName><Initials>CW</Initials></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Kwok Hung</ForeName><Initials>KH</Initials></Author><Author ValidYN="Y"><LastName>Peiris</LastName><ForeName>Joseph S Malik</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Leung</LastName><ForeName>Frederick Chi Ching</ForeName><Initials>FC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000906">Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin 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MajorTopicYN="N">Antibodies</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000917" MajorTopicYN="N">Antibody Formation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007111" MajorTopicYN="N">Immunity, Cellular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007114" MajorTopicYN="N">Immunization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021122" MajorTopicYN="N">Protein Subunits</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS 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|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:14985131
|texte= Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:14985131" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |