Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
Identifieur interne : 002D96 ( PubMed/Checkpoint ); précédent : 002D95; suivant : 002D97Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
Auteurs : Fanya Zeng [République populaire de Chine] ; Ken Yan Ching Chow ; Chung Chau Hon ; Ka Man Law ; Chi Wai Yip ; Kwok Hung Chan ; Joseph S Malik Peiris ; Frederick Chi Ching LeungSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Anticorps (immunologie), Expression des gènes, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Immunisation, Immunité cellulaire, Immunoglobuline G (immunologie), Plasmides (génétique), Plasmides (immunologie), Production d'anticorps (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Sous-unités de protéines (immunologie), Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- génétique : Glycoprotéines membranaires, Plasmides, Protéines de l'enveloppe virale, Protéines recombinantes, Vaccins à ADN, Virus du SRAS.
- immunologie : Anticorps, Glycoprotéines membranaires, Immunoglobuline G, Plasmides, Protéines de l'enveloppe virale, Protéines recombinantes, Sous-unités de protéines, Vaccins à ADN, Virus du SRAS.
- Animaux, Expression des gènes, Glycoprotéine de spicule des coronavirus, Immunisation, Immunité cellulaire, Production d'anticorps, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies (immunology), Antibody Formation (drug effects), Gene Expression, Immunity, Cellular, Immunization, Immunoglobulin G (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Plasmids (genetics), Plasmids (immunology), Protein Subunits (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Vaccines, DNA (genetics), Vaccines, DNA (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Immunoglobulin G, Membrane Glycoproteins, Protein Subunits, Recombinant Proteins, Vaccines, DNA, Viral Envelope Proteins.
- drug effects : Antibody Formation.
- genetics : Plasmids, SARS Virus.
- immunology : Plasmids, SARS Virus.
- Animals, Gene Expression, Immunity, Cellular, Immunization, Mice, Mice, Inbred BALB C, Spike Glycoprotein, Coronavirus.
Abstract
The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.
DOI: 10.1016/j.bbrc.2004.01.166
PubMed: 14985131
Affiliations:
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pubmed:14985131Le document en format XML
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<front><div type="abstract" xml:lang="en">The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.</div>
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<Abstract><AbstractText>The immunological characteristics of SARS-CoV spike protein were investigated by administering mice with plasmids encoding various S gene fragments. We showed that the secreting forms of S1, S2 subunits and the N-terminus of S1 subunit (residues 18-495) were capable of eliciting SARS-CoV specific antibodies and the region immediate to N-terminus of matured S1 protein contained an important immunogenic determinant for elicitation of SARS-CoV specific antibodies. In addition, mice immunized with plasmids encoding S1 fragment developed a Th1-mediated antibody isotype switching. Another interesting finding was that mouse antibodies elicited separately by plasmids encoding S1 and S2 subunits cooperatively neutralized SARS-CoV but neither the S1 nor S2 specific antibodies did, suggesting the possible role of both S1 and S2 subunits in host cell docking and entry. These results provide insights into understanding the immunological characteristics of spike protein and the development of subunit vaccines against SARS-CoV.</AbstractText>
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<affiliations><list><country><li>République populaire de Chine</li>
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<name sortKey="Chow, Ken Yan Ching" sort="Chow, Ken Yan Ching" uniqKey="Chow K" first="Ken Yan Ching" last="Chow">Ken Yan Ching Chow</name>
<name sortKey="Hon, Chung Chau" sort="Hon, Chung Chau" uniqKey="Hon C" first="Chung Chau" last="Hon">Chung Chau Hon</name>
<name sortKey="Law, Ka Man" sort="Law, Ka Man" uniqKey="Law K" first="Ka Man" last="Law">Ka Man Law</name>
<name sortKey="Leung, Frederick Chi Ching" sort="Leung, Frederick Chi Ching" uniqKey="Leung F" first="Frederick Chi Ching" last="Leung">Frederick Chi Ching Leung</name>
<name sortKey="Peiris, Joseph S Malik" sort="Peiris, Joseph S Malik" uniqKey="Peiris J" first="Joseph S Malik" last="Peiris">Joseph S Malik Peiris</name>
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