Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells.
Identifieur interne : 001282 ( Ncbi/Checkpoint ); précédent : 001281; suivant : 001283Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells.
Auteurs : Ken Y C. Chow [République populaire de Chine] ; Yin Shan Yeung ; Chung Chau Hon ; Fanya Zeng ; Ka Man Law ; Frederick C C. LeungSource :
- FEBS letters [ 0014-5793 ] ; 2005.
Descripteurs français
- KwdFr :
- Adenoviridae (génétique), Adenoviridae (métabolisme), Animaux, Apoptose, Cellules Vero, Facteurs temps, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Immunohistochimie, Lignée cellulaire, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Structure tertiaire des protéines, Survie cellulaire, Syndrome respiratoire aigu sévère (virologie), Transduction génétique, Vecteurs génétiques.
- MESH :
- génétique : Adenoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- métabolisme : Adenoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Apoptose, Cellules Vero, Facteurs temps, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Immunohistochimie, Lignée cellulaire, Protéines de l'enveloppe virale, Structure tertiaire des protéines, Survie cellulaire, Transduction génétique, Vecteurs génétiques.
English descriptors
- KwdEn :
- Adenoviridae (genetics), Adenoviridae (metabolism), Animals, Apoptosis, Cell Line, Cell Survival, Chlorocebus aethiops, Genetic Vectors, Humans, Immunohistochemistry, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Protein Structure, Tertiary, Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Time Factors, Transduction, Genetic, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Adenoviridae, Membrane Glycoproteins, Viral Envelope Proteins.
- metabolism : Adenoviridae, Membrane Glycoproteins, Viral Envelope Proteins.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Apoptosis, Cell Line, Cell Survival, Chlorocebus aethiops, Genetic Vectors, Humans, Immunohistochemistry, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus, Time Factors, Transduction, Genetic, Vero Cells.
Abstract
The pro-apoptotic properties of severe acute respiratory syndrome coronavirus (SARS-CoV) structural proteins were studied in vitro. By monitoring apoptosis indicators including chromatin condensation, cellular DNA fragmentation and cell membrane asymmetry, we demonstrated that the adenovirus-mediated over-expression of SARS-CoV spike (S) protein and its C-terminal domain (S2) induce apoptosis in Vero E6 cells in a time- and dosage-dependent manner, whereas the expression of its N-terminal domain (S1) and other structural proteins, including envelope (E), membrane (M) and nucleocapsid (N) protein do not. These findings suggest a possible role of S and S2 protein in SARS-CoV induced apoptosis and the molecular pathogenesis of SARS.
DOI: 10.1016/j.febslet.2005.10.065
PubMed: 16310778
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002430
- to stream PubMed, to step Curation: 002430
- to stream PubMed, to step Checkpoint: 002844
- to stream Ncbi, to step Merge: 001282
- to stream Ncbi, to step Curation: 001282
Links to Exploration step
pubmed:16310778Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells.</title><author><name sortKey="Chow, Ken Y C" sort="Chow, Ken Y C" uniqKey="Chow K" first="Ken Y C" last="Chow">Ken Y C. Chow</name><affiliation wicri:level="1"><nlm:affiliation>Department of Zoology, Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong SAR</wicri:regionArea><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Yeung, Yin Shan" sort="Yeung, Yin Shan" uniqKey="Yeung Y" first="Yin Shan" last="Yeung">Yin Shan Yeung</name></author><author><name sortKey="Hon, Chung Chau" sort="Hon, Chung Chau" uniqKey="Hon C" first="Chung Chau" last="Hon">Chung Chau Hon</name></author><author><name sortKey="Zeng, Fanya" sort="Zeng, Fanya" uniqKey="Zeng F" first="Fanya" last="Zeng">Fanya Zeng</name></author><author><name sortKey="Law, Ka Man" sort="Law, Ka Man" uniqKey="Law K" first="Ka Man" last="Law">Ka Man Law</name></author><author><name sortKey="Leung, Frederick C C" sort="Leung, Frederick C C" uniqKey="Leung F" first="Frederick C C" last="Leung">Frederick C C. Leung</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16310778</idno><idno type="pmid">16310778</idno><idno type="doi">10.1016/j.febslet.2005.10.065</idno><idno type="wicri:Area/PubMed/Corpus">002430</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002430</idno><idno type="wicri:Area/PubMed/Curation">002430</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002430</idno><idno type="wicri:Area/PubMed/Checkpoint">002844</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002844</idno><idno type="wicri:Area/Ncbi/Merge">001282</idno><idno type="wicri:Area/Ncbi/Curation">001282</idno><idno type="wicri:Area/Ncbi/Checkpoint">001282</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells.</title><author><name sortKey="Chow, Ken Y C" sort="Chow, Ken Y C" uniqKey="Chow K" first="Ken Y C" last="Chow">Ken Y C. Chow</name><affiliation wicri:level="1"><nlm:affiliation>Department of Zoology, Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Zoology, Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong SAR</wicri:regionArea><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Yeung, Yin Shan" sort="Yeung, Yin Shan" uniqKey="Yeung Y" first="Yin Shan" last="Yeung">Yin Shan Yeung</name></author><author><name sortKey="Hon, Chung Chau" sort="Hon, Chung Chau" uniqKey="Hon C" first="Chung Chau" last="Hon">Chung Chau Hon</name></author><author><name sortKey="Zeng, Fanya" sort="Zeng, Fanya" uniqKey="Zeng F" first="Fanya" last="Zeng">Fanya Zeng</name></author><author><name sortKey="Law, Ka Man" sort="Law, Ka Man" uniqKey="Law K" first="Ka Man" last="Law">Ka Man Law</name></author><author><name sortKey="Leung, Frederick C C" sort="Leung, Frederick C C" uniqKey="Leung F" first="Frederick C C" last="Leung">Frederick C C. Leung</name></author></analytic><series><title level="j">FEBS letters</title><idno type="ISSN">0014-5793</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae (genetics)</term><term>Adenoviridae (metabolism)</term><term>Animals</term><term>Apoptosis</term><term>Cell Line</term><term>Cell Survival</term><term>Chlorocebus aethiops</term><term>Genetic Vectors</term><term>Humans</term><term>Immunohistochemistry</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>Protein Structure, Tertiary</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Time Factors</term><term>Transduction, Genetic</term><term>Vero Cells</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adenoviridae (génétique)</term><term>Adenoviridae (métabolisme)</term><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Facteurs temps</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Immunohistochimie</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Structure tertiaire des protéines</term><term>Survie cellulaire</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Transduction génétique</term><term>Vecteurs génétiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviridae</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adenoviridae</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Adenoviridae</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adenoviridae</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Cell Line</term><term>Cell Survival</term><term>Chlorocebus aethiops</term><term>Genetic Vectors</term><term>Humans</term><term>Immunohistochemistry</term><term>Protein Structure, Tertiary</term><term>Spike Glycoprotein, Coronavirus</term><term>Time Factors</term><term>Transduction, Genetic</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Facteurs temps</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Immunohistochimie</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale</term><term>Structure tertiaire des protéines</term><term>Survie cellulaire</term><term>Transduction génétique</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pro-apoptotic properties of severe acute respiratory syndrome coronavirus (SARS-CoV) structural proteins were studied in vitro. By monitoring apoptosis indicators including chromatin condensation, cellular DNA fragmentation and cell membrane asymmetry, we demonstrated that the adenovirus-mediated over-expression of SARS-CoV spike (S) protein and its C-terminal domain (S2) induce apoptosis in Vero E6 cells in a time- and dosage-dependent manner, whereas the expression of its N-terminal domain (S1) and other structural proteins, including envelope (E), membrane (M) and nucleocapsid (N) protein do not. These findings suggest a possible role of S and S2 protein in SARS-CoV induced apoptosis and the molecular pathogenesis of SARS.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Hon, Chung Chau" sort="Hon, Chung Chau" uniqKey="Hon C" first="Chung Chau" last="Hon">Chung Chau Hon</name><name sortKey="Law, Ka Man" sort="Law, Ka Man" uniqKey="Law K" first="Ka Man" last="Law">Ka Man Law</name><name sortKey="Leung, Frederick C C" sort="Leung, Frederick C C" uniqKey="Leung F" first="Frederick C C" last="Leung">Frederick C C. Leung</name><name sortKey="Yeung, Yin Shan" sort="Yeung, Yin Shan" uniqKey="Yeung Y" first="Yin Shan" last="Yeung">Yin Shan Yeung</name><name sortKey="Zeng, Fanya" sort="Zeng, Fanya" uniqKey="Zeng F" first="Fanya" last="Zeng">Fanya Zeng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Chow, Ken Y C" sort="Chow, Ken Y C" uniqKey="Chow K" first="Ken Y C" last="Chow">Ken Y C. Chow</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001282 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 001282 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:16310778
|texte= Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:16310778" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |