The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: expression and functional characterization.
Identifieur interne : 001283 ( Ncbi/Checkpoint ); précédent : 001282; suivant : 001284The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: expression and functional characterization.
Auteurs : Hua Geng [République populaire de Chine] ; Yuet-Man Liu ; Wai-Sing Chan ; Anthony Wing-Ip Lo ; Deborah Mun-Yee Au ; Mary Miu-Yee Waye ; Yuan-Yuan HoSource :
- FEBS letters [ 0014-5793 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN (biosynthèse), Animaux, Cadres ouverts de lecture, Cellules CHO, Cellules Vero, Cricetinae, Données de séquences moléculaires, Expression des gènes, Humains, Immunohistochimie, Intestins (anatomopathologie), Lignée cellulaire, Microscopie confocale, Muqueuse intestinale (métabolisme), Noyau de la cellule (métabolisme), Poumon (anatomopathologie), Poumon (métabolisme), Protéines de fusion recombinantes (), Protéines de fusion recombinantes (métabolisme), Protéines virales (), Protéines virales (génétique), Protéines virales (métabolisme), Protéines à fluorescence verte (métabolisme), Réticulum endoplasmique (métabolisme), Syndrome respiratoire aigu sévère (métabolisme), Syndrome respiratoire aigu sévère (virologie), Séquence d'acides aminés, Virus du SRAS (), Études cas-témoins.
- MESH :
- anatomopathologie : Intestins, Poumon.
- biosynthèse : ADN.
- génétique : Protéines virales.
- métabolisme : Muqueuse intestinale, Noyau de la cellule, Poumon, Protéines de fusion recombinantes, Protéines virales, Protéines à fluorescence verte, Réticulum endoplasmique, Syndrome respiratoire aigu sévère.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cadres ouverts de lecture, Cellules CHO, Cellules Vero, Cricetinae, Données de séquences moléculaires, Expression des gènes, Humains, Immunohistochimie, Lignée cellulaire, Microscopie confocale, Protéines de fusion recombinantes, Protéines virales, Séquence d'acides aminés, Virus du SRAS, Études cas-témoins.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, CHO Cells, Case-Control Studies, Cell Line, Cell Nucleus (metabolism), Chlorocebus aethiops, Cricetinae, DNA (biosynthesis), Endoplasmic Reticulum (metabolism), Gene Expression, Green Fluorescent Proteins (metabolism), Humans, Immunohistochemistry, Intestinal Mucosa (metabolism), Intestines (pathology), Lung (metabolism), Lung (pathology), Microscopy, Confocal, Molecular Sequence Data, Open Reading Frames, Recombinant Fusion Proteins (chemistry), Recombinant Fusion Proteins (metabolism), SARS Virus (chemistry), Severe Acute Respiratory Syndrome (metabolism), Severe Acute Respiratory Syndrome (virology), Vero Cells, Viral Proteins (chemistry), Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , biosynthesis : DNA.
- chemical , chemistry : Recombinant Fusion Proteins, Viral Proteins.
- chemistry : SARS Virus.
- chemical , genetics : Viral Proteins.
- metabolism : Cell Nucleus, Endoplasmic Reticulum, Green Fluorescent Proteins, Intestinal Mucosa, Lung, Recombinant Fusion Proteins, Severe Acute Respiratory Syndrome, Viral Proteins.
- pathology : Intestines, Lung.
- virology : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, CHO Cells, Case-Control Studies, Cell Line, Chlorocebus aethiops, Cricetinae, Gene Expression, Humans, Immunohistochemistry, Microscopy, Confocal, Molecular Sequence Data, Open Reading Frames, Vero Cells.
Abstract
The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.
DOI: 10.1016/j.febslet.2005.11.007
PubMed: 16310783
Affiliations:
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>CHO Cells</term>
<term>Case-Control Studies</term>
<term>Cell Line</term>
<term>Cell Nucleus (metabolism)</term>
<term>Chlorocebus aethiops</term>
<term>Cricetinae</term>
<term>DNA (biosynthesis)</term>
<term>Endoplasmic Reticulum (metabolism)</term>
<term>Gene Expression</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>Intestinal Mucosa (metabolism)</term>
<term>Intestines (pathology)</term>
<term>Lung (metabolism)</term>
<term>Lung (pathology)</term>
<term>Microscopy, Confocal</term>
<term>Molecular Sequence Data</term>
<term>Open Reading Frames</term>
<term>Recombinant Fusion Proteins (chemistry)</term>
<term>Recombinant Fusion Proteins (metabolism)</term>
<term>SARS Virus (chemistry)</term>
<term>Severe Acute Respiratory Syndrome (metabolism)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Vero Cells</term>
<term>Viral Proteins (chemistry)</term>
<term>Viral Proteins (genetics)</term>
<term>Viral Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (biosynthèse)</term>
<term>Animaux</term>
<term>Cadres ouverts de lecture</term>
<term>Cellules CHO</term>
<term>Cellules Vero</term>
<term>Cricetinae</term>
<term>Données de séquences moléculaires</term>
<term>Expression des gènes</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Intestins (anatomopathologie)</term>
<term>Lignée cellulaire</term>
<term>Microscopie confocale</term>
<term>Muqueuse intestinale (métabolisme)</term>
<term>Noyau de la cellule (métabolisme)</term>
<term>Poumon (anatomopathologie)</term>
<term>Poumon (métabolisme)</term>
<term>Protéines de fusion recombinantes ()</term>
<term>Protéines de fusion recombinantes (métabolisme)</term>
<term>Protéines virales ()</term>
<term>Protéines virales (génétique)</term>
<term>Protéines virales (métabolisme)</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Réticulum endoplasmique (métabolisme)</term>
<term>Syndrome respiratoire aigu sévère (métabolisme)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Séquence d'acides aminés</term>
<term>Virus du SRAS ()</term>
<term>Études cas-témoins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>DNA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Recombinant Fusion Proteins</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Intestins</term>
<term>Poumon</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term>
<term>Endoplasmic Reticulum</term>
<term>Green Fluorescent Proteins</term>
<term>Intestinal Mucosa</term>
<term>Lung</term>
<term>Recombinant Fusion Proteins</term>
<term>Severe Acute Respiratory Syndrome</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Muqueuse intestinale</term>
<term>Noyau de la cellule</term>
<term>Poumon</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines virales</term>
<term>Protéines à fluorescence verte</term>
<term>Réticulum endoplasmique</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Intestines</term>
<term>Lung</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>CHO Cells</term>
<term>Case-Control Studies</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Cricetinae</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>Microscopy, Confocal</term>
<term>Molecular Sequence Data</term>
<term>Open Reading Frames</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cadres ouverts de lecture</term>
<term>Cellules CHO</term>
<term>Cellules Vero</term>
<term>Cricetinae</term>
<term>Données de séquences moléculaires</term>
<term>Expression des gènes</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Lignée cellulaire</term>
<term>Microscopie confocale</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines virales</term>
<term>Séquence d'acides aminés</term>
<term>Virus du SRAS</term>
<term>Études cas-témoins</term>
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<front><div type="abstract" xml:lang="en">The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.</div>
</front>
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<affiliations><list><country><li>République populaire de Chine</li>
</country>
<settlement><li>Sha Tin</li>
</settlement>
<orgName><li>Université chinoise de Hong Kong</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Au, Deborah Mun Yee" sort="Au, Deborah Mun Yee" uniqKey="Au D" first="Deborah Mun-Yee" last="Au">Deborah Mun-Yee Au</name>
<name sortKey="Chan, Wai Sing" sort="Chan, Wai Sing" uniqKey="Chan W" first="Wai-Sing" last="Chan">Wai-Sing Chan</name>
<name sortKey="Ho, Yuan Yuan" sort="Ho, Yuan Yuan" uniqKey="Ho Y" first="Yuan-Yuan" last="Ho">Yuan-Yuan Ho</name>
<name sortKey="Liu, Yuet Man" sort="Liu, Yuet Man" uniqKey="Liu Y" first="Yuet-Man" last="Liu">Yuet-Man Liu</name>
<name sortKey="Lo, Anthony Wing Ip" sort="Lo, Anthony Wing Ip" uniqKey="Lo A" first="Anthony Wing-Ip" last="Lo">Anthony Wing-Ip Lo</name>
<name sortKey="Waye, Mary Miu Yee" sort="Waye, Mary Miu Yee" uniqKey="Waye M" first="Mary Miu-Yee" last="Waye">Mary Miu-Yee Waye</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Geng, Hua" sort="Geng, Hua" uniqKey="Geng H" first="Hua" last="Geng">Hua Geng</name>
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