Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells.
Identifieur interne : 001282 ( Ncbi/Curation ); précédent : 001281; suivant : 001283Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells.
Auteurs : Ken Y C. Chow [République populaire de Chine] ; Yin Shan Yeung ; Chung Chau Hon ; Fanya Zeng ; Ka Man Law ; Frederick C C. LeungSource :
- FEBS letters [ 0014-5793 ] ; 2005.
Descripteurs français
- KwdFr :
- Adenoviridae (génétique), Adenoviridae (métabolisme), Animaux, Apoptose, Cellules Vero, Facteurs temps, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Immunohistochimie, Lignée cellulaire, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Structure tertiaire des protéines, Survie cellulaire, Syndrome respiratoire aigu sévère (virologie), Transduction génétique, Vecteurs génétiques.
- MESH :
- génétique : Adenoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- métabolisme : Adenoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Apoptose, Cellules Vero, Facteurs temps, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Immunohistochimie, Lignée cellulaire, Protéines de l'enveloppe virale, Structure tertiaire des protéines, Survie cellulaire, Transduction génétique, Vecteurs génétiques.
English descriptors
- KwdEn :
- Adenoviridae (genetics), Adenoviridae (metabolism), Animals, Apoptosis, Cell Line, Cell Survival, Chlorocebus aethiops, Genetic Vectors, Humans, Immunohistochemistry, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Protein Structure, Tertiary, Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Time Factors, Transduction, Genetic, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Adenoviridae, Membrane Glycoproteins, Viral Envelope Proteins.
- metabolism : Adenoviridae, Membrane Glycoproteins, Viral Envelope Proteins.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Apoptosis, Cell Line, Cell Survival, Chlorocebus aethiops, Genetic Vectors, Humans, Immunohistochemistry, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus, Time Factors, Transduction, Genetic, Vero Cells.
Abstract
The pro-apoptotic properties of severe acute respiratory syndrome coronavirus (SARS-CoV) structural proteins were studied in vitro. By monitoring apoptosis indicators including chromatin condensation, cellular DNA fragmentation and cell membrane asymmetry, we demonstrated that the adenovirus-mediated over-expression of SARS-CoV spike (S) protein and its C-terminal domain (S2) induce apoptosis in Vero E6 cells in a time- and dosage-dependent manner, whereas the expression of its N-terminal domain (S1) and other structural proteins, including envelope (E), membrane (M) and nucleocapsid (N) protein do not. These findings suggest a possible role of S and S2 protein in SARS-CoV induced apoptosis and the molecular pathogenesis of SARS.
DOI: 10.1016/j.febslet.2005.10.065
PubMed: 16310778
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pubmed:16310778Le document en format XML
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<term>Adenoviridae (metabolism)</term>
<term>Animals</term>
<term>Apoptosis</term>
<term>Cell Line</term>
<term>Cell Survival</term>
<term>Chlorocebus aethiops</term>
<term>Genetic Vectors</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Protein Structure, Tertiary</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Time Factors</term>
<term>Transduction, Genetic</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
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<term>Adenoviridae (métabolisme)</term>
<term>Animaux</term>
<term>Apoptose</term>
<term>Cellules Vero</term>
<term>Facteurs temps</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Lignée cellulaire</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Structure tertiaire des protéines</term>
<term>Survie cellulaire</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Transduction génétique</term>
<term>Vecteurs génétiques</term>
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<term>Viral Envelope Proteins</term>
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<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
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<term>Protéines de l'enveloppe virale</term>
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<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
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<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
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<term>Apoptosis</term>
<term>Cell Line</term>
<term>Cell Survival</term>
<term>Chlorocebus aethiops</term>
<term>Genetic Vectors</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
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<term>Time Factors</term>
<term>Transduction, Genetic</term>
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<term>Apoptose</term>
<term>Cellules Vero</term>
<term>Facteurs temps</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
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<term>Immunohistochimie</term>
<term>Lignée cellulaire</term>
<term>Protéines de l'enveloppe virale</term>
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<front><div type="abstract" xml:lang="en">The pro-apoptotic properties of severe acute respiratory syndrome coronavirus (SARS-CoV) structural proteins were studied in vitro. By monitoring apoptosis indicators including chromatin condensation, cellular DNA fragmentation and cell membrane asymmetry, we demonstrated that the adenovirus-mediated over-expression of SARS-CoV spike (S) protein and its C-terminal domain (S2) induce apoptosis in Vero E6 cells in a time- and dosage-dependent manner, whereas the expression of its N-terminal domain (S1) and other structural proteins, including envelope (E), membrane (M) and nucleocapsid (N) protein do not. These findings suggest a possible role of S and S2 protein in SARS-CoV induced apoptosis and the molecular pathogenesis of SARS.</div>
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