Adenoviral expression of a truncated S1 subunit of SARS-CoV spike protein results in specific humoral immune responses against SARS-CoV in rats.
Identifieur interne : 001062 ( Ncbi/Checkpoint ); précédent : 001061; suivant : 001063Adenoviral expression of a truncated S1 subunit of SARS-CoV spike protein results in specific humoral immune responses against SARS-CoV in rats.
Auteurs : Ran-Yi Liu [République populaire de Chine] ; Li-Zhi Wu ; Bi-Jun Huang ; Jia-Ling Huang ; Yan-Ling Zhang ; Miao-La Ke ; Jun-Mei Wang ; Wei-Ping Tan ; Ru-Hua Zhang ; Han-Kui Chen ; Yi-Xin Zeng ; Wenlin HuangSource :
- Virus research [ 0168-1702 ] ; 2005.
Descripteurs français
- KwdFr :
- Adenoviridae (génétique), Adenoviridae (métabolisme), Animaux, Anticorps antiviraux (sang), Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Humains, Lignée cellulaire, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Rat Wistar, Rats, Spécificité des anticorps, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Tests de neutralisation, Vaccination, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vecteurs génétiques, Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Vaccins antiviraux.
- génétique : Adenoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins antiviraux, Virus du SRAS.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- métabolisme : Adenoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- sang : Anticorps antiviraux.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Lignée cellulaire, Protéines de l'enveloppe virale, Rat Wistar, Rats, Spécificité des anticorps, Syndrome respiratoire aigu sévère, Tests de neutralisation, Vaccination, Vecteurs génétiques.
English descriptors
- KwdEn :
- Adenoviridae (genetics), Adenoviridae (metabolism), Animals, Antibodies, Viral (blood), Antibody Specificity, Cell Line, Chlorocebus aethiops, Genetic Vectors, Humans, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Neutralization Tests, Rats, Rats, Wistar, SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Vaccination, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , blood : Antibodies, Viral.
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Adenoviridae, Membrane Glycoproteins, SARS Virus, Viral Envelope Proteins, Viral Vaccines.
- chemical , immunology : Membrane Glycoproteins, SARS Virus, Severe Acute Respiratory Syndrome, Viral Envelope Proteins, Viral Vaccines.
- metabolism : Adenoviridae, Membrane Glycoproteins, Viral Envelope Proteins.
- prevention & control : Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Antibody Specificity, Cell Line, Chlorocebus aethiops, Genetic Vectors, Humans, Neutralization Tests, Rats, Rats, Wistar, Spike Glycoprotein, Coronavirus, Vaccination, Vero Cells.
Abstract
The causative agent of severe acute respiratory syndrome (SARS) has been identified as SARS-associated coronavirus (SARS-CoV), but the prophylactic treatment of SARS-CoV is still under investigation. We constructed a recombinant adenovirus containing a truncated N-terminal fragment of the SARS-CoV Spike (S) gene (from--45 to 1469, designated Ad-S(N)), which encoded a truncated S protein (490 amino-acid residues, a part of 672 amino-acid S1 subunit), and investigated whether this construct could induce effective immunity against SARS-CoV in Wistar rats. Rats were immunized either subcutaneously or intranasally with Ad-S(N) once a week for three consecutive weeks. Our results showed that all of the immunized animals generated humoral immunity against the SARS-CoV spike protein, and the sera of immunized rats showed strong capable of protecting from SARS-CoV infection in vitro. Histopathological examination did not find evident side effects in the immunized animals. These results indicate that an adenoviral-based vaccine carrying an N-terminal fragment of the Spike gene is able to elicit strong SARS-CoV-specific humoral immune responses in rats, and may be useful for the development of a protective vaccine against SARS-CoV infection.
DOI: 10.1016/j.virusres.2005.02.009
PubMed: 16022898
Affiliations:
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last="Tan">Wei-Ping Tan</name></author><author><name sortKey="Zhang, Ru Hua" sort="Zhang, Ru Hua" uniqKey="Zhang R" first="Ru-Hua" last="Zhang">Ru-Hua Zhang</name></author><author><name sortKey="Chen, Han Kui" sort="Chen, Han Kui" uniqKey="Chen H" first="Han-Kui" last="Chen">Han-Kui Chen</name></author><author><name sortKey="Zeng, Yi Xin" sort="Zeng, Yi Xin" uniqKey="Zeng Y" first="Yi-Xin" last="Zeng">Yi-Xin Zeng</name></author><author><name sortKey="Huang, Wenlin" sort="Huang, Wenlin" uniqKey="Huang W" first="Wenlin" last="Huang">Wenlin Huang</name></author></analytic><series><title level="j">Virus research</title><idno type="ISSN">0168-1702</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae (genetics)</term><term>Adenoviridae (metabolism)</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Antibody 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Glycoprotein, Coronavirus</term><term>Vaccination</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale</term><term>Rat Wistar</term><term>Rats</term><term>Spécificité des anticorps</term><term>Syndrome respiratoire aigu sévère</term><term>Tests de neutralisation</term><term>Vaccination</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The causative agent of severe acute respiratory syndrome (SARS) has been identified as SARS-associated coronavirus (SARS-CoV), but the prophylactic treatment of SARS-CoV is still under investigation. We constructed a recombinant adenovirus containing a truncated N-terminal fragment of the SARS-CoV Spike (S) gene (from--45 to 1469, designated Ad-S(N)), which encoded a truncated S protein (490 amino-acid residues, a part of 672 amino-acid S1 subunit), and investigated whether this construct could induce effective immunity against SARS-CoV in Wistar rats. Rats were immunized either subcutaneously or intranasally with Ad-S(N) once a week for three consecutive weeks. Our results showed that all of the immunized animals generated humoral immunity against the SARS-CoV spike protein, and the sera of immunized rats showed strong capable of protecting from SARS-CoV infection in vitro. Histopathological examination did not find evident side effects in the immunized animals. These results indicate that an adenoviral-based vaccine carrying an N-terminal fragment of the Spike gene is able to elicit strong SARS-CoV-specific humoral immune responses in rats, and may be useful for the development of a protective vaccine against SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Chen, Han Kui" sort="Chen, Han Kui" uniqKey="Chen H" first="Han-Kui" last="Chen">Han-Kui Chen</name><name sortKey="Huang, Bi Jun" sort="Huang, Bi Jun" uniqKey="Huang B" first="Bi-Jun" last="Huang">Bi-Jun Huang</name><name sortKey="Huang, Jia Ling" sort="Huang, Jia Ling" uniqKey="Huang J" first="Jia-Ling" last="Huang">Jia-Ling Huang</name><name sortKey="Huang, Wenlin" sort="Huang, Wenlin" uniqKey="Huang W" first="Wenlin" last="Huang">Wenlin Huang</name><name sortKey="Ke, Miao La" sort="Ke, Miao La" uniqKey="Ke M" first="Miao-La" last="Ke">Miao-La Ke</name><name sortKey="Tan, Wei Ping" sort="Tan, Wei Ping" uniqKey="Tan W" first="Wei-Ping" last="Tan">Wei-Ping Tan</name><name sortKey="Wang, Jun Mei" sort="Wang, Jun Mei" uniqKey="Wang J" first="Jun-Mei" last="Wang">Jun-Mei Wang</name><name sortKey="Wu, Li Zhi" sort="Wu, Li Zhi" uniqKey="Wu L" first="Li-Zhi" last="Wu">Li-Zhi Wu</name><name sortKey="Zeng, Yi Xin" sort="Zeng, Yi Xin" uniqKey="Zeng Y" first="Yi-Xin" last="Zeng">Yi-Xin Zeng</name><name sortKey="Zhang, Ru Hua" sort="Zhang, Ru Hua" uniqKey="Zhang R" first="Ru-Hua" last="Zhang">Ru-Hua Zhang</name><name sortKey="Zhang, Yan Ling" sort="Zhang, Yan Ling" uniqKey="Zhang Y" first="Yan-Ling" last="Zhang">Yan-Ling Zhang</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Liu, Ran Yi" sort="Liu, Ran Yi" uniqKey="Liu R" first="Ran-Yi" last="Liu">Ran-Yi Liu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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