Thermal aggregation of SARS-CoV membrane protein.
Identifieur interne : 001063 ( Ncbi/Checkpoint ); précédent : 001062; suivant : 001064Thermal aggregation of SARS-CoV membrane protein.
Auteurs : Yi-Nung Lee [Taïwan] ; Li-Kuang Chen ; Hsin-Chieh Ma ; Hui-Hua Yang ; Hsin-Pai Li ; Shih-Yen LoSource :
- Journal of virological methods [ 0166-0934 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Protéines de la matrice virale.
- isolement et purification : Protéines de la matrice virale.
- métabolisme : Protéines de la matrice virale.
- Animaux, Cellules Vero, Dénaturation des protéines, Technique de Western, Température élevée, Transfection, Virus du SRAS.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Viral Matrix Proteins.
- chemistry : SARS Virus.
- chemical , isolation & purification : Viral Matrix Proteins.
- chemical , metabolism : Viral Matrix Proteins.
- methods : Blotting, Western.
- Animals, Chlorocebus aethiops, Hot Temperature, Protein Denaturation, Transfection, Vero Cells.
Abstract
SARS-CoV membrane protein could be detected easily using Western blotting in non-denaturing condition but not regular denaturing treatment. Boiling treatment, causing the aggregation of SARS-CoV membrane protein in the stacking gels, results in the failure to detect the membrane protein in the separating gels. Aggregated membrane proteins could not be dissociated by 1% Triton-X 100, 6M urea, or 2% SDS. The region with amino acid residues from 51 to 170 is responsible for thermal aggregation of SARS-CoV membrane protein. Hydrophobic regions with amino acid residues from 61 to 90, from 91 to 100, from 136 to 170, are essential for this protein aggregation. Thermal aggregation of SARS-CoV membrane protein is not unique among structural proteins of coronaviruses. However, SARS-CoV membrane protein seems to be more sensitive to heat treatment, since the membrane protein of MHV-JHM, another member of the Coronaviridae, would not aggregate after the same treatment. Therefore, if SARS-CoV membrane protein needs to be analyzed using SDS-PAGE, boiling should be avoided. Thermal aggregation of SARS-CoV membrane protein may be one of the reasons for the inactivation of this virus by heat. The unusual property of SARS-CoV membrane protein aggregation induced by heat also provides a model for the study of protein aggregation.
DOI: 10.1016/j.jviromet.2005.05.022
PubMed: 16023741
Affiliations:
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Yang</name></author><author><name sortKey="Li, Hsin Pai" sort="Li, Hsin Pai" uniqKey="Li H" first="Hsin-Pai" last="Li">Hsin-Pai Li</name></author><author><name sortKey="Lo, Shih Yen" sort="Lo, Shih Yen" uniqKey="Lo S" first="Shih-Yen" last="Lo">Shih-Yen Lo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16023741</idno><idno type="pmid">16023741</idno><idno type="doi">10.1016/j.jviromet.2005.05.022</idno><idno type="wicri:Area/PubMed/Corpus">002638</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002638</idno><idno type="wicri:Area/PubMed/Curation">002638</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002638</idno><idno type="wicri:Area/PubMed/Checkpoint">002412</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002412</idno><idno type="wicri:Area/Ncbi/Merge">001063</idno><idno 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Ma</name></author><author><name sortKey="Yang, Hui Hua" sort="Yang, Hui Hua" uniqKey="Yang H" first="Hui-Hua" last="Yang">Hui-Hua Yang</name></author><author><name sortKey="Li, Hsin Pai" sort="Li, Hsin Pai" uniqKey="Li H" first="Hsin-Pai" last="Li">Hsin-Pai Li</name></author><author><name sortKey="Lo, Shih Yen" sort="Lo, Shih Yen" uniqKey="Lo S" first="Shih-Yen" last="Lo">Shih-Yen Lo</name></author></analytic><series><title level="j">Journal of virological methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western (methods)</term><term>Chlorocebus aethiops</term><term>Hot Temperature</term><term>Protein Denaturation</term><term>SARS Virus (chemistry)</term><term>Transfection</term><term>Vero Cells</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Matrix Proteins (isolation & purification)</term><term>Viral Matrix Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Dénaturation des protéines</term><term>Protéines de la matrice virale (génétique)</term><term>Protéines de la matrice virale (isolement et purification)</term><term>Protéines de la matrice virale (métabolisme)</term><term>Technique de Western ()</term><term>Température élevée</term><term>Transfection</term><term>Virus du SRAS ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de la matrice virale</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines de la matrice virale</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Blotting, Western</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de la matrice virale</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Hot Temperature</term><term>Protein Denaturation</term><term>Transfection</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Dénaturation des protéines</term><term>Technique de Western</term><term>Température élevée</term><term>Transfection</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">SARS-CoV membrane protein could be detected easily using Western blotting in non-denaturing condition but not regular denaturing treatment. Boiling treatment, causing the aggregation of SARS-CoV membrane protein in the stacking gels, results in the failure to detect the membrane protein in the separating gels. Aggregated membrane proteins could not be dissociated by 1% Triton-X 100, 6M urea, or 2% SDS. The region with amino acid residues from 51 to 170 is responsible for thermal aggregation of SARS-CoV membrane protein. Hydrophobic regions with amino acid residues from 61 to 90, from 91 to 100, from 136 to 170, are essential for this protein aggregation. Thermal aggregation of SARS-CoV membrane protein is not unique among structural proteins of coronaviruses. However, SARS-CoV membrane protein seems to be more sensitive to heat treatment, since the membrane protein of MHV-JHM, another member of the Coronaviridae, would not aggregate after the same treatment. Therefore, if SARS-CoV membrane protein needs to be analyzed using SDS-PAGE, boiling should be avoided. Thermal aggregation of SARS-CoV membrane protein may be one of the reasons for the inactivation of this virus by heat. The unusual property of SARS-CoV membrane protein aggregation induced by heat also provides a model for the study of protein aggregation.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Chen, Li Kuang" sort="Chen, Li Kuang" uniqKey="Chen L" first="Li-Kuang" last="Chen">Li-Kuang Chen</name><name sortKey="Li, Hsin Pai" sort="Li, Hsin Pai" uniqKey="Li H" first="Hsin-Pai" last="Li">Hsin-Pai Li</name><name sortKey="Lo, Shih Yen" sort="Lo, Shih Yen" uniqKey="Lo S" first="Shih-Yen" last="Lo">Shih-Yen Lo</name><name sortKey="Ma, Hsin Chieh" sort="Ma, Hsin Chieh" uniqKey="Ma H" first="Hsin-Chieh" last="Ma">Hsin-Chieh Ma</name><name sortKey="Yang, Hui Hua" sort="Yang, Hui Hua" uniqKey="Yang H" first="Hui-Hua" last="Yang">Hui-Hua Yang</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Lee, Yi Nung" sort="Lee, Yi Nung" uniqKey="Lee Y" first="Yi-Nung" last="Lee">Yi-Nung Lee</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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