SARS Coronavirus Detection Methods
Identifieur interne : 001061 ( Ncbi/Checkpoint ); précédent : 001060; suivant : 001062SARS Coronavirus Detection Methods
Auteurs : Susanna K. P. Lau [République populaire de Chine] ; Xiao-Yan Che [République populaire de Chine] ; Patrick C. Y. Woo [République populaire de Chine] ; Beatrice H. L. Wong [République populaire de Chine] ; Vincent C. C. Cheng [République populaire de Chine] ; Gibson K. S. Woo [République populaire de Chine] ; Ivan F. N. Hung [République populaire de Chine] ; Rosana W. S. Poon [République populaire de Chine] ; Kwok-Hung Chan [République populaire de Chine] ; J. S. Malik Peiris [République populaire de Chine] ; Kwok-Yung Yuen [République populaire de Chine]Source :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- isolement et purification : Protéines nucléocapside, Virus du SRAS.
- virologie : Fèces.
- Humains, RT-PCR, Sensibilité et spécificité, Syndrome respiratoire aigu sévère, Test ELISA.
English descriptors
- KwdEn :
- Enzyme-Linked Immunosorbent Assay (methods), Feces (virology), Humans, Nucleocapsid Proteins (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (urine).
- MESH :
- chemical , isolation & purification : Nucleocapsid Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- methods : Enzyme-Linked Immunosorbent Assay, Reverse Transcriptase Polymerase Chain Reaction.
- urine : Severe Acute Respiratory Syndrome.
- virology : Feces.
- Humans, Sensitivity and Specificity.
Abstract
Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.
Url:
DOI: 10.3201/eid1107.041045
PubMed: 16022791
PubMed Central: 3371792
Affiliations:
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PMC:3371792Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.</p>
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