SARS Coronavirus Detection Methods
Identifieur interne : 001252 ( Pmc/Checkpoint ); précédent : 001251; suivant : 001253SARS Coronavirus Detection Methods
Auteurs : Susanna K. P. Lau [République populaire de Chine] ; Xiao-Yan Che [République populaire de Chine] ; Patrick C. Y. Woo [République populaire de Chine] ; Beatrice H. L. Wong [République populaire de Chine] ; Vincent C. C. Cheng [République populaire de Chine] ; Gibson K. S. Woo [République populaire de Chine] ; Ivan F. N. Hung [République populaire de Chine] ; Rosana W. S. Poon [République populaire de Chine] ; Kwok-Hung Chan [République populaire de Chine] ; J. S. Malik Peiris [République populaire de Chine] ; Kwok-Yung Yuen [République populaire de Chine]Source :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2005.
Abstract
Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.
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DOI: 10.3201/eid1107.041045
PubMed: 16022791
PubMed Central: 3371792
Affiliations:
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Lau</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation wicri:level="3"><nlm:aff id="aff2">First Military Medical University, Guangzhou, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>First Military Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C. C." last="Cheng">Vincent C. C. Cheng</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Woo, Gibson K S" sort="Woo, Gibson K S" uniqKey="Woo G" first="Gibson K. S." last="Woo">Gibson K. S. Woo</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F. N." last="Hung">Ivan F. N. Hung</name><affiliation wicri:level="1"><nlm:aff id="aff3">Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Queen Mary Hospital, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W. S." last="Poon">Rosana W. S. Poon</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16022791</idno><idno type="pmc">3371792</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371792</idno><idno type="RBID">PMC:3371792</idno><idno type="doi">10.3201/eid1107.041045</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000866</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000866</idno><idno type="wicri:Area/Pmc/Curation">000866</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000866</idno><idno type="wicri:Area/Pmc/Checkpoint">001252</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001252</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">SARS Coronavirus Detection Methods</title><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation wicri:level="3"><nlm:aff id="aff2">First Military Medical University, Guangzhou, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>First Military Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C. C." last="Cheng">Vincent C. C. Cheng</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Woo, Gibson K S" sort="Woo, Gibson K S" uniqKey="Woo G" first="Gibson K. S." last="Woo">Gibson K. S. Woo</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F. N." last="Hung">Ivan F. N. Hung</name><affiliation wicri:level="1"><nlm:aff id="aff3">Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Queen Mary Hospital, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W. S." last="Poon">Rosana W. S. Poon</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author></analytic><series><title level="j">Emerging Infectious Diseases</title><idno type="ISSN">1080-6040</idno><idno type="eISSN">1080-6059</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Emerg Infect Dis</journal-id><journal-id journal-id-type="iso-abbrev">Emerging Infect. Dis</journal-id><journal-id journal-id-type="publisher-id">EID</journal-id><journal-title-group><journal-title>Emerging Infectious Diseases</journal-title></journal-title-group><issn pub-type="ppub">1080-6040</issn><issn pub-type="epub">1080-6059</issn><publisher><publisher-name>Centers for Disease Control and Prevention</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16022791</article-id><article-id pub-id-type="pmc">3371792</article-id><article-id pub-id-type="publisher-id">04-1045</article-id><article-id pub-id-type="doi">10.3201/eid1107.041045</article-id><article-categories><subj-group subj-group-type="heading"><subject>Dispatch</subject></subj-group><subj-group subj-group-type="article-type"><subject>Dispatch</subject></subj-group></article-categories><title-group><article-title>SARS Coronavirus Detection Methods</article-title><alt-title alt-title-type="running-head">ELISA versus qRT-PCR for SARS-CoV detection</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lau</surname><given-names>Susanna K.P.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Che</surname><given-names>Xiao-Yan</given-names></name><xref ref-type="aff" rid="aff2">†</xref></contrib><contrib contrib-type="author"><name><surname>Woo</surname><given-names>Patrick C.Y.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Wong</surname><given-names>Beatrice H.L.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Cheng</surname><given-names>Vincent C.C.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Woo</surname><given-names>Gibson K.S.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Hung</surname><given-names>Ivan F.N.</given-names></name><xref ref-type="aff" rid="aff3">‡</xref></contrib><contrib contrib-type="author"><name><surname>Poon</surname><given-names>Rosana W.S.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Kwok-Hung</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Peiris</surname><given-names>J.S. Malik</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Yuen</surname><given-names>Kwok-Yung</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><aff id="aff1"><label>*</label>University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</aff><aff id="aff2"><label>†</label>First Military Medical University, Guangzhou, People's Republic of China;</aff><aff id="aff3"><label>‡</label>Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China</aff></contrib-group><author-notes><corresp id="cor1">Address for correspondence: Kwok-Yung Yuen, Department of Microbiology, University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong, People's Republic of China; fax: 852-2855-1241; email: <email xlink:href="hkumicro@hkucc.hku.hk">hkumicro@hkucc.hku.hk</email></corresp></author-notes><pub-date pub-type="ppub"><month>7</month><year>2005</year></pub-date><volume>11</volume><issue>7</issue><fpage>1108</fpage><lpage>1111</lpage><abstract><p>Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.</p></abstract><kwd-group kwd-group-type="author"><title>Keywords: </title><kwd>SARS-Cov</kwd><kwd>qRT-PCR</kwd><kwd>nucleocapsid</kwd><kwd>capture ELISA</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name></noRegion><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C. C." last="Cheng">Vincent C. C. Cheng</name><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F. N." last="Hung">Ivan F. N. Hung</name><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W. S." last="Poon">Rosana W. S. Poon</name><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><name sortKey="Woo, Gibson K S" sort="Woo, Gibson K S" uniqKey="Woo G" first="Gibson K. S." last="Woo">Gibson K. S. Woo</name><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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