SARS Coronavirus Detection Methods
Identifieur interne : 001252 ( Pmc/Checkpoint ); précédent : 001251; suivant : 001253SARS Coronavirus Detection Methods
Auteurs : Susanna K. P. Lau [République populaire de Chine] ; Xiao-Yan Che [République populaire de Chine] ; Patrick C. Y. Woo [République populaire de Chine] ; Beatrice H. L. Wong [République populaire de Chine] ; Vincent C. C. Cheng [République populaire de Chine] ; Gibson K. S. Woo [République populaire de Chine] ; Ivan F. N. Hung [République populaire de Chine] ; Rosana W. S. Poon [République populaire de Chine] ; Kwok-Hung Chan [République populaire de Chine] ; J. S. Malik Peiris [République populaire de Chine] ; Kwok-Yung Yuen [République populaire de Chine]Source :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2005.
Abstract
Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.
Url:
DOI: 10.3201/eid1107.041045
PubMed: 16022791
PubMed Central: 3371792
Affiliations:
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<country xml:lang="fr">République populaire de Chine</country>
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<author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea>
<wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion>
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<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea>
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<front><div type="abstract" xml:lang="en"><p>Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Emerg Infect Dis</journal-id>
<journal-id journal-id-type="iso-abbrev">Emerging Infect. Dis</journal-id>
<journal-id journal-id-type="publisher-id">EID</journal-id>
<journal-title-group><journal-title>Emerging Infectious Diseases</journal-title>
</journal-title-group>
<issn pub-type="ppub">1080-6040</issn>
<issn pub-type="epub">1080-6059</issn>
<publisher><publisher-name>Centers for Disease Control and Prevention</publisher-name>
</publisher>
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<article-meta><article-id pub-id-type="pmid">16022791</article-id>
<article-id pub-id-type="pmc">3371792</article-id>
<article-id pub-id-type="publisher-id">04-1045</article-id>
<article-id pub-id-type="doi">10.3201/eid1107.041045</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Dispatch</subject>
</subj-group>
<subj-group subj-group-type="article-type"><subject>Dispatch</subject>
</subj-group>
</article-categories>
<title-group><article-title>SARS Coronavirus Detection Methods</article-title>
<alt-title alt-title-type="running-head">ELISA versus qRT-PCR for SARS-CoV detection</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Lau</surname>
<given-names>Susanna K.P.</given-names>
</name>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Che</surname>
<given-names>Xiao-Yan</given-names>
</name>
<xref ref-type="aff" rid="aff2">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Woo</surname>
<given-names>Patrick C.Y.</given-names>
</name>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wong</surname>
<given-names>Beatrice H.L.</given-names>
</name>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Cheng</surname>
<given-names>Vincent C.C.</given-names>
</name>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Woo</surname>
<given-names>Gibson K.S.</given-names>
</name>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hung</surname>
<given-names>Ivan F.N.</given-names>
</name>
<xref ref-type="aff" rid="aff3">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Poon</surname>
<given-names>Rosana W.S.</given-names>
</name>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chan</surname>
<given-names>Kwok-Hung</given-names>
</name>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Peiris</surname>
<given-names>J.S. Malik</given-names>
</name>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Yuen</surname>
<given-names>Kwok-Yung</given-names>
</name>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<aff id="aff1"><label>*</label>
University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</aff>
<aff id="aff2"><label>†</label>
First Military Medical University, Guangzhou, People's Republic of China;</aff>
<aff id="aff3"><label>‡</label>
Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China</aff>
</contrib-group>
<author-notes><corresp id="cor1">Address for correspondence: Kwok-Yung Yuen, Department of Microbiology, University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong, People's Republic of China; fax: 852-2855-1241; email: <email xlink:href="hkumicro@hkucc.hku.hk">hkumicro@hkucc.hku.hk</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>7</month>
<year>2005</year>
</pub-date>
<volume>11</volume>
<issue>7</issue>
<fpage>1108</fpage>
<lpage>1111</lpage>
<abstract><p>Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.</p>
</abstract>
<kwd-group kwd-group-type="author"><title>Keywords: </title>
<kwd>SARS-Cov</kwd>
<kwd>qRT-PCR</kwd>
<kwd>nucleocapsid</kwd>
<kwd>capture ELISA</kwd>
</kwd-group>
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<affiliations><list><country><li>République populaire de Chine</li>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name>
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<name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
<name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name>
<name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C. C." last="Cheng">Vincent C. C. Cheng</name>
<name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F. N." last="Hung">Ivan F. N. Hung</name>
<name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name>
<name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W. S." last="Poon">Rosana W. S. Poon</name>
<name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name>
<name sortKey="Woo, Gibson K S" sort="Woo, Gibson K S" uniqKey="Woo G" first="Gibson K. S." last="Woo">Gibson K. S. Woo</name>
<name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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