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SARS coronavirus detection methods.

Identifieur interne : 002640 ( PubMed/Curation ); précédent : 002639; suivant : 002641

SARS coronavirus detection methods.

Auteurs : Susanna K P. Lau [République populaire de Chine] ; Xiao-Yan Che ; Patrick C Y. Woo ; Beatrice H L. Wong ; Vincent C C. Cheng ; Gibson K S. Woo ; Ivan F N. Hung ; Rosana W S. Poon ; Kwok-Hung Chan ; J S Malik Peiris ; Kwok-Yung Yuen

Source :

Emerging infectious diseases [ 1080-6040 ] ; 2005.

RBID : pubmed:16022791

Descripteurs français

KwdFr :
- Fèces (virologie), Humains, Protéines nucléocapside (isolement et purification), RT-PCR (), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (urine), Test ELISA (), Virus du SRAS (isolement et purification).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- isolement et purification : Protéines nucléocapside, Virus du SRAS.
- virologie : Fèces.
- Humains, RT-PCR, Sensibilité et spécificité, Syndrome respiratoire aigu sévère, Test ELISA.

English descriptors

KwdEn :
- Enzyme-Linked Immunosorbent Assay (methods), Feces (virology), Humans, Nucleocapsid Proteins (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (urine).
MESH :
- chemical , isolation & purification : Nucleocapsid Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- methods : Enzyme-Linked Immunosorbent Assay, Reverse Transcriptase Polymerase Chain Reaction.
- urine : Severe Acute Respiratory Syndrome.
- virology : Feces.
- Humans, Sensitivity and Specificity.

Abstract

Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription-polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.

DOI: 10.3201/eid1107.041045
PubMed: 16022791

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pubmed:16022791

Le document en format XML

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<author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K P" last="Lau">Susanna K P. Lau</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea>
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<author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name>
</author>
<author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C Y" last="Woo">Patrick C Y. Woo</name>
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<author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H L" last="Wong">Beatrice H L. Wong</name>
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<author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C C" last="Cheng">Vincent C C. Cheng</name>
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<author><name sortKey="Woo, Gibson K S" sort="Woo, Gibson K S" uniqKey="Woo G" first="Gibson K S" last="Woo">Gibson K S. Woo</name>
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<author><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F N" last="Hung">Ivan F N. Hung</name>
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<author><name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W S" last="Poon">Rosana W S. Poon</name>
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<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
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<author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J S Malik" last="Peiris">J S Malik Peiris</name>
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<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China.</nlm:affiliation>
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<author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name>
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<author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H L" last="Wong">Beatrice H L. Wong</name>
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<author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C C" last="Cheng">Vincent C C. Cheng</name>
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<author><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F N" last="Hung">Ivan F N. Hung</name>
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<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
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<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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<term>Nucleocapsid Proteins (isolation & purification)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (urine)</term>
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<term>Protéines nucléocapside (isolement et purification)</term>
<term>RT-PCR ()</term>
<term>Sensibilité et spécificité</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (urine)</term>
<term>Test ELISA ()</term>
<term>Virus du SRAS (isolement et purification)</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid Proteins</term>
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<front><div type="abstract" xml:lang="en">Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription-polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.</div>
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SARS coronavirus detection methods.

SARS coronavirus detection methods.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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