SrasV1, Main, Merge, bibRecord, 005193

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Identifieur interne : 005193 ( Main/Merge ); précédent : 005192; suivant : 005194

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Auteurs : Yong-Hua Li [République populaire de Chine] ; JIE LI [République populaire de Chine] ; Xue-En Liu [République populaire de Chine] ; LING WANG [République populaire de Chine] ; TONG LI [République populaire de Chine] ; Yi-Hua Zhou [République populaire de Chine] ; HUI ZHUANG [République populaire de Chine]

Source :

Journal of virological methods [ 0166-0934 ] ; 2005.

RBID : Pascal:06-0005624

Descripteurs français

Pascal (Inist)
- Coronavirus, Détection, Nucléocapside, Protéine, Sérum, Microbiologie, Méthode, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Detection, Method, Microbiology, Nucleocapsid, Protein, Serum, Severe acute respiratory syndrome, Virology.

Abstract

A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Detection</term>
<term>Method</term>
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<term>Protein</term>
<term>Serum</term>
<term>Severe acute respiratory syndrome</term>
<term>Virology</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
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<front><div type="abstract" xml:lang="en">A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.</div>
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Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri