Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers
Identifieur interne : 000418 ( PascalFrancis/Curation ); précédent : 000417; suivant : 000419Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers
Auteurs : Yong-Hua Li [République populaire de Chine] ; JIE LI [République populaire de Chine] ; Xue-En Liu [République populaire de Chine] ; LING WANG [République populaire de Chine] ; TONG LI [République populaire de Chine] ; Yi-Hua Zhou [République populaire de Chine] ; HUI ZHUANG [République populaire de Chine]Source :
- Journal of virological methods [ 0166-0934 ] ; 2005.
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- Pascal (Inist)
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Abstract
A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.
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<front><div type="abstract" xml:lang="en">A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.</div>
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<fC07 i1="05" i2="X" l="SPA"><s0>Infección</s0>
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