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Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Identifieur interne : 000418 ( PascalFrancis/Curation ); précédent : 000417; suivant : 000419

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Auteurs : Yong-Hua Li [République populaire de Chine] ; JIE LI [République populaire de Chine] ; Xue-En Liu [République populaire de Chine] ; LING WANG [République populaire de Chine] ; TONG LI [République populaire de Chine] ; Yi-Hua Zhou [République populaire de Chine] ; HUI ZHUANG [République populaire de Chine]

Source :

RBID : Pascal:06-0005624

Descripteurs français

English descriptors

Abstract

A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.
pA  
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A03   1    @0 J. virol. methods
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A08 01  1  ENG  @1 Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers
A11 01  1    @1 LI (Yong-Hua)
A11 02  1    @1 JIE LI
A11 03  1    @1 LIU (Xue-En)
A11 04  1    @1 LING WANG
A11 05  1    @1 TONG LI
A11 06  1    @1 ZHOU (Yi-Hua)
A11 07  1    @1 HUI ZHUANG
A14 01      @1 Department of Microbiology, Peking University Health Science Center, 38 Xueyuan Road @2 Beijing 100083 @3 CHN @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut.
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A44       @0 0000 @1 © 2006 INIST-CNRS. All rights reserved.
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A60       @1 P
A61       @0 A
A64 01  1    @0 Journal of virological methods
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C01 01    ENG  @0 A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.
C02 01  X    @0 002A05C09
C03 01  X  FRE  @0 Coronavirus @2 NW @5 01
C03 01  X  ENG  @0 Coronavirus @2 NW @5 01
C03 01  X  SPA  @0 Coronavirus @2 NW @5 01
C03 02  X  FRE  @0 Détection @5 05
C03 02  X  ENG  @0 Detection @5 05
C03 02  X  SPA  @0 Detección @5 05
C03 03  X  FRE  @0 Nucléocapside @5 06
C03 03  X  ENG  @0 Nucleocapsid @5 06
C03 03  X  SPA  @0 Nucleocápside @5 06
C03 04  X  FRE  @0 Protéine @5 07
C03 04  X  ENG  @0 Protein @5 07
C03 04  X  SPA  @0 Proteína @5 07
C03 05  X  FRE  @0 Sérum @5 08
C03 05  X  ENG  @0 Serum @5 08
C03 05  X  SPA  @0 Suero @5 08
C03 06  X  FRE  @0 Microbiologie @5 09
C03 06  X  ENG  @0 Microbiology @5 09
C03 06  X  SPA  @0 Microbiología @5 09
C03 07  X  FRE  @0 Méthode @5 10
C03 07  X  ENG  @0 Method @5 10
C03 07  X  SPA  @0 Método @5 10
C03 08  X  FRE  @0 Virologie @5 11
C03 08  X  ENG  @0 Virology @5 11
C03 08  X  SPA  @0 Virología @5 11
C03 09  X  FRE  @0 Syndrome respiratoire aigu sévère @2 NM @5 14
C03 09  X  ENG  @0 Severe acute respiratory syndrome @2 NM @5 14
C03 09  X  SPA  @0 Síndrome respiratorio agudo severo @2 NM @5 14
C07 01  X  FRE  @0 Coronaviridae @2 NW
C07 01  X  ENG  @0 Coronaviridae @2 NW
C07 01  X  SPA  @0 Coronaviridae @2 NW
C07 02  X  FRE  @0 Nidovirales @2 NW
C07 02  X  ENG  @0 Nidovirales @2 NW
C07 02  X  SPA  @0 Nidovirales @2 NW
C07 03  X  FRE  @0 Virus @2 NW
C07 03  X  ENG  @0 Virus @2 NW
C07 03  X  SPA  @0 Virus @2 NW
C07 04  X  FRE  @0 Virose
C07 04  X  ENG  @0 Viral disease
C07 04  X  SPA  @0 Virosis
C07 05  X  FRE  @0 Infection
C07 05  X  ENG  @0 Infection
C07 05  X  SPA  @0 Infección
N21       @1 002
N44 01      @1 OTO
N82       @1 OTO

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Pascal:06-0005624

Le document en format XML

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<div type="abstract" xml:lang="en">A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.</div>
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<fC03 i1="01" i2="X" l="SPA">
<s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE">
<s0>Détection</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG">
<s0>Detection</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA">
<s0>Detección</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE">
<s0>Nucléocapside</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Nucleocapsid</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Nucleocápside</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Protéine</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Protein</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Proteína</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Sérum</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Serum</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Suero</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE">
<s0>Microbiologie</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG">
<s0>Microbiology</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA">
<s0>Microbiología</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE">
<s0>Méthode</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG">
<s0>Method</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA">
<s0>Método</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE">
<s0>Virologie</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG">
<s0>Virology</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA">
<s0>Virología</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE">
<s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG">
<s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA">
<s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Virose</s0>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Viral disease</s0>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Virosis</s0>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Infection</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Infection</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Infección</s0>
</fC07>
<fN21>
<s1>002</s1>
</fN21>
<fN44 i1="01">
<s1>OTO</s1>
</fN44>
<fN82>
<s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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   |wiki=    Sante
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   |flux=    PascalFrancis
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   |texte=   Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers
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