SrasV1, PascalFrancis, Corpus, bibRecord, 000572

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Identifieur interne : 000572 ( PascalFrancis/Corpus ); précédent : 000571; suivant : 000573

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Auteurs : Yong-Hua Li ; JIE LI ; Xue-En Liu ; LING WANG ; TONG LI ; Yi-Hua Zhou ; HUI ZHUANG

Source :

Journal of virological methods [ 0166-0934 ] ; 2005.

RBID : Pascal:06-0005624

Descripteurs français

Pascal (Inist)
- Coronavirus, Détection, Nucléocapside, Protéine, Sérum, Microbiologie, Méthode, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Detection, Method, Microbiology, Nucleocapsid, Protein, Serum, Severe acute respiratory syndrome, Virology.

Abstract

A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`01`	`1`		`@1 LI (Yong-Hua)`
A11	`02`	`1`		`@1 JIE LI`
A11	`03`	`1`		`@1 LIU (Xue-En)`
A11	`04`	`1`		`@1 LING WANG`
A11	`05`	`1`		`@1 TONG LI`
A11	`06`	`1`		`@1 ZHOU (Yi-Hua)`
A11	`07`	`1`		`@1 HUI ZHUANG`
A14	`01`			`@1 Department of Microbiology, Peking University Health Science Center, 38 Xueyuan Road @2 Beijing 100083 @3 CHN @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut.`
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C01	`01`		`ENG`	@0 A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.
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C03	`06`	`X`	`ENG`	`@0 Microbiology @5 09`
C03	`06`	`X`	`SPA`	`@0 Microbiología @5 09`
C03	`07`	`X`	`FRE`	`@0 Méthode @5 10`
C03	`07`	`X`	`ENG`	`@0 Method @5 10`
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C03	`09`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 14`
C03	`09`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
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C07	`02`	`X`	`FRE`	`@0 Nidovirales @2 NW`
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N21				`@1 002`
N44	`01`			`@1 OTO`
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Format Inist (serveur)

NO :	PASCAL 06-0005624 INIST
ET :	Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers
AU :	LI (Yong-Hua); JIE LI; LIU (Xue-En); LING WANG; TONG LI; ZHOU (Yi-Hua); HUI ZHUANG
AF :	Department of Microbiology, Peking University Health Science Center, 38 Xueyuan Road/Beijing 100083/Chine (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of virological methods; ISSN 0166-0934; Coden JVMEDH; Pays-Bas; Da. 2005; Vol. 130; No. 1-2; Pp. 45-50; Bibl. 1 p.1/4
LA :	Anglais
EA :	A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.
CC :	002A05C09
FD :	Coronavirus; Détection; Nucléocapside; Protéine; Sérum; Microbiologie; Méthode; Virologie; Syndrome respiratoire aigu sévère
FG :	Coronaviridae; Nidovirales; Virus; Virose; Infection
ED :	Coronavirus; Detection; Nucleocapsid; Protein; Serum; Microbiology; Method; Virology; Severe acute respiratory syndrome
EG :	Coronaviridae; Nidovirales; Virus; Viral disease; Infection
SD :	Coronavirus; Detección; Nucleocápside; Proteína; Suero; Microbiología; Método; Virología; Síndrome respiratorio agudo severo
LO :	INIST-18295.354000135504060070
ID :	06-0005624

Links to Exploration step

Pascal:06-0005624

Le document en format XML

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<front><div type="abstract" xml:lang="en">A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.</div>
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<s5>01</s5>
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<s5>05</s5>
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<s5>07</s5>
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<s5>08</s5>
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<s5>11</s5>
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<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
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<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
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<s2>NW</s2>
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<s2>NW</s2>
</fC07>
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<s2>NW</s2>
</fC07>
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<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
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<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fN21><s1>002</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
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<server><NO>PASCAL 06-0005624 INIST</NO>
<ET>Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers</ET>
<AU>LI (Yong-Hua); JIE LI; LIU (Xue-En); LING WANG; TONG LI; ZHOU (Yi-Hua); HUI ZHUANG</AU>
<AF>Department of Microbiology, Peking University Health Science Center, 38 Xueyuan Road/Beijing 100083/Chine (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virological methods; ISSN 0166-0934; Coden JVMEDH; Pays-Bas; Da. 2005; Vol. 130; No. 1-2; Pp. 45-50; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.</EA>
<CC>002A05C09</CC>
<FD>Coronavirus; Détection; Nucléocapside; Protéine; Sérum; Microbiologie; Méthode; Virologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Virose; Infection</FG>
<ED>Coronavirus; Detection; Nucleocapsid; Protein; Serum; Microbiology; Method; Virology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Viral disease; Infection</EG>
<SD>Coronavirus; Detección; Nucleocápside; Proteína; Suero; Microbiología; Método; Virología; Síndrome respiratorio agudo severo</SD>
<LO>INIST-18295.354000135504060070</LO>
<ID>06-0005624</ID>
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Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

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