A severe acute respiratory syndrome-associated coronavirus-specific protein enhances virulence of an attenuated murine coronavirus
Identifieur interne : 004E85 ( Main/Exploration ); précédent : 004E84; suivant : 004E86A severe acute respiratory syndrome-associated coronavirus-specific protein enhances virulence of an attenuated murine coronavirus
Auteurs : Lecia Pewe [États-Unis] ; HAIXIA ZHOU [États-Unis] ; Jason Netland [États-Unis] ; Chandra Tangudu [États-Unis] ; Heidi Olivares [États-Unis] ; LEI SHI [États-Unis] ; Dwight Look [États-Unis] ; Thomas Gallagher [États-Unis] ; Stanley Perlman [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cadres ouverts de lecture (génétique), Encéphalite (virologie), Humains, Infections à coronavirus (virologie), Lignée cellulaire, Modèles animaux de maladie humaine, Protéines virales non structurales (génétique), Protéines virales non structurales (métabolisme), Réplication virale, Souris, Souris de lignée C57BL, Virulence, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (pathogénicité), Virus du SRAS (), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie), Virus recombinants (pathogénicité).
- MESH :
- génétique : Cadres ouverts de lecture, Protéines virales non structurales, Virus de l'hépatite murine.
- métabolisme : Protéines virales non structurales.
- pathogénicité : Virus de l'hépatite murine, Virus du SRAS, Virus recombinants.
- physiologie : Virus du SRAS.
- virologie : Encéphalite, Infections à coronavirus.
- Pascal (Inist)
English descriptors
- KwdEn :
- Animals, Cell Line, Coronavirus, Coronavirus Infections (virology), Disease Models, Animal, Encephalitis (virology), Humans, Mice, Mice, Inbred C57BL, Microbiology, Murine hepatitis virus, Murine hepatitis virus (genetics), Murine hepatitis virus (pathogenicity), Open Reading Frames (genetics), Protein, Reassortant Viruses (pathogenicity), SARS Virus (chemistry), SARS Virus (pathogenicity), SARS Virus (physiology), Severe acute respiratory syndrome, Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism), Virology, Virulence, Virus Replication.
- MESH :
- chemical , genetics : Viral Nonstructural Proteins.
- chemistry : SARS Virus.
- genetics : Murine hepatitis virus, Open Reading Frames.
- chemical , metabolism : Viral Nonstructural Proteins.
- pathogenicity : Murine hepatitis virus, Reassortant Viruses, SARS Virus.
- physiology : SARS Virus.
- virology : Coronavirus Infections, Encephalitis.
- Animals, Cell Line, Disease Models, Animal, Humans, Mice, Mice, Inbred C57BL, Virulence, Virus Replication.
Abstract
Most animal species that can be infected with the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) do not reproducibly develop clinical disease, hindering studies of pathogenesis. To develop an alternative system for the study of SARS-CoV, we introduced individual SARS-CoV genes (open reading frames [ORFs]) into the genome of an attenuated murine coronavirus. One protein, the product of SARS-CoV ORF6, converted a sublethal infection to a uniformly lethal encephalitis and enhanced virus growth in tissue culture cells, indicating that SARS-CoV proteins function in the context of a heterologous coronavirus infection. Furthermore, these results suggest that the attenuated murine coronavirus lacks a virulence gene residing in SARS-CoV. Recombinant murine coronaviruses cause a reproducible and well-characterized clinical disease, offer virtually no risk to laboratory personnel, and should be useful for elucidating the role of SARS-CoV nonstructural proteins in viral replication and pathogenesis.
Url:
Affiliations:
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Le document en format XML
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<term>Cell Line</term>
<term>Coronavirus</term>
<term>Coronavirus Infections (virology)</term>
<term>Disease Models, Animal</term>
<term>Encephalitis (virology)</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Microbiology</term>
<term>Murine hepatitis virus</term>
<term>Murine hepatitis virus (genetics)</term>
<term>Murine hepatitis virus (pathogenicity)</term>
<term>Open Reading Frames (genetics)</term>
<term>Protein</term>
<term>Reassortant Viruses (pathogenicity)</term>
<term>SARS Virus (chemistry)</term>
<term>SARS Virus (pathogenicity)</term>
<term>SARS Virus (physiology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Viral Nonstructural Proteins (genetics)</term>
<term>Viral Nonstructural Proteins (metabolism)</term>
<term>Virology</term>
<term>Virulence</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cadres ouverts de lecture (génétique)</term>
<term>Encéphalite (virologie)</term>
<term>Humains</term>
<term>Infections à coronavirus (virologie)</term>
<term>Lignée cellulaire</term>
<term>Modèles animaux de maladie humaine</term>
<term>Protéines virales non structurales (génétique)</term>
<term>Protéines virales non structurales (métabolisme)</term>
<term>Réplication virale</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Virulence</term>
<term>Virus de l'hépatite murine (génétique)</term>
<term>Virus de l'hépatite murine (pathogénicité)</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (pathogénicité)</term>
<term>Virus du SRAS (physiologie)</term>
<term>Virus recombinants (pathogénicité)</term>
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<term>Protéines virales non structurales</term>
<term>Virus de l'hépatite murine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Nonstructural Proteins</term>
</keywords>
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</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Murine hepatitis virus</term>
<term>Reassortant Viruses</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de l'hépatite murine</term>
<term>Virus du SRAS</term>
<term>Virus recombinants</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Encéphalite</term>
<term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Encephalitis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Disease Models, Animal</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Virulence</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Coronavirus</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Modèles animaux de maladie humaine</term>
<term>Réplication virale</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Virulence</term>
<term>Virus du SRAS</term>
<term>Virus hépatite murine</term>
<term>Protéine</term>
<term>Virulence</term>
<term>Microbiologie</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Most animal species that can be infected with the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) do not reproducibly develop clinical disease, hindering studies of pathogenesis. To develop an alternative system for the study of SARS-CoV, we introduced individual SARS-CoV genes (open reading frames [ORFs]) into the genome of an attenuated murine coronavirus. One protein, the product of SARS-CoV ORF6, converted a sublethal infection to a uniformly lethal encephalitis and enhanced virus growth in tissue culture cells, indicating that SARS-CoV proteins function in the context of a heterologous coronavirus infection. Furthermore, these results suggest that the attenuated murine coronavirus lacks a virulence gene residing in SARS-CoV. Recombinant murine coronaviruses cause a reproducible and well-characterized clinical disease, offer virtually no risk to laboratory personnel, and should be useful for elucidating the role of SARS-CoV nonstructural proteins in viral replication and pathogenesis.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Iowa</li>
</region>
<settlement><li>Iowa City</li>
</settlement>
<orgName><li>Université de l'Iowa</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Iowa"><name sortKey="Pewe, Lecia" sort="Pewe, Lecia" uniqKey="Pewe L" first="Lecia" last="Pewe">Lecia Pewe</name>
</region>
<name sortKey="Gallagher, Thomas" sort="Gallagher, Thomas" uniqKey="Gallagher T" first="Thomas" last="Gallagher">Thomas Gallagher</name>
<name sortKey="Haixia Zhou" sort="Haixia Zhou" uniqKey="Haixia Zhou" last="Haixia Zhou">HAIXIA ZHOU</name>
<name sortKey="Lei Shi" sort="Lei Shi" uniqKey="Lei Shi" last="Lei Shi">LEI SHI</name>
<name sortKey="Look, Dwight" sort="Look, Dwight" uniqKey="Look D" first="Dwight" last="Look">Dwight Look</name>
<name sortKey="Netland, Jason" sort="Netland, Jason" uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name>
<name sortKey="Olivares, Heidi" sort="Olivares, Heidi" uniqKey="Olivares H" first="Heidi" last="Olivares">Heidi Olivares</name>
<name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<name sortKey="Tangudu, Chandra" sort="Tangudu, Chandra" uniqKey="Tangudu C" first="Chandra" last="Tangudu">Chandra Tangudu</name>
</country>
</tree>
</affiliations>
</record>
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