A Severe Acute Respiratory Syndrome-Associated Coronavirus-Specific Protein Enhances Virulence of an Attenuated Murine Coronavirus
Identifieur interne : 004C84 ( Main/Merge ); précédent : 004C83; suivant : 004C85A Severe Acute Respiratory Syndrome-Associated Coronavirus-Specific Protein Enhances Virulence of an Attenuated Murine Coronavirus
Auteurs : Lecia Pewe ; Haixia Zhou ; Jason Netland ; Chandra Tangudu ; Heidi Olivares ; Lei Shi ; Dwight Look ; Thomas Gallagher ; Stanley PerlmanSource :
- Journal of Virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cadres ouverts de lecture (génétique), Encéphalite (virologie), Humains, Infections à coronavirus (virologie), Lignée cellulaire, Modèles animaux de maladie humaine, Protéines virales non structurales (génétique), Protéines virales non structurales (métabolisme), Réplication virale, Souris, Souris de lignée C57BL, Virulence, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (pathogénicité), Virus du SRAS (), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie), Virus recombinants (pathogénicité).
- MESH :
- génétique : Cadres ouverts de lecture, Protéines virales non structurales, Virus de l'hépatite murine.
- métabolisme : Protéines virales non structurales.
- pathogénicité : Virus de l'hépatite murine, Virus du SRAS, Virus recombinants.
- physiologie : Virus du SRAS.
- virologie : Encéphalite, Infections à coronavirus.
- Animaux, Humains, Lignée cellulaire, Modèles animaux de maladie humaine, Réplication virale, Souris, Souris de lignée C57BL, Virulence, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Cell Line, Coronavirus Infections (virology), Disease Models, Animal, Encephalitis (virology), Humans, Mice, Mice, Inbred C57BL, Murine hepatitis virus (genetics), Murine hepatitis virus (pathogenicity), Open Reading Frames (genetics), Reassortant Viruses (pathogenicity), SARS Virus (chemistry), SARS Virus (pathogenicity), SARS Virus (physiology), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism), Virulence, Virus Replication.
- MESH :
- chemical , genetics : Viral Nonstructural Proteins.
- chemistry : SARS Virus.
- genetics : Murine hepatitis virus, Open Reading Frames.
- chemical , metabolism : Viral Nonstructural Proteins.
- pathogenicity : Murine hepatitis virus, Reassortant Viruses, SARS Virus.
- physiology : SARS Virus.
- virology : Coronavirus Infections, Encephalitis.
- Animals, Cell Line, Disease Models, Animal, Humans, Mice, Mice, Inbred C57BL, Virulence, Virus Replication.
Abstract
Most animal species that can be infected with the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) do not reproducibly develop clinical disease, hindering studies of pathogenesis. To develop an alternative system for the study of SARS-CoV, we introduced individual SARS-CoV genes (open reading frames [ORFs]) into the genome of an attenuated murine coronavirus. One protein, the product of SARS-CoV ORF6, converted a sublethal infection to a uniformly lethal encephalitis and enhanced virus growth in tissue culture cells, indicating that SARS-CoV proteins function in the context of a heterologous coronavirus infection. Furthermore, these results suggest that the attenuated murine coronavirus lacks a virulence gene residing in SARS-CoV. Recombinant murine coronaviruses cause a reproducible and well-characterized clinical disease, offer virtually no risk to laboratory personnel, and should be useful for elucidating the role of SARS-CoV nonstructural proteins in viral replication and pathogenesis.
Url:
DOI: 10.1128/JVI.79.17.11335-11342.2005
PubMed: 16103185
PubMed Central: 1193615
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000696
- to stream Pmc, to step Curation: 000696
- to stream Pmc, to step Checkpoint: 001407
- to stream PubMed, to step Corpus: 002586
- to stream PubMed, to step Curation: 002586
- to stream PubMed, to step Checkpoint: 002853
- to stream Ncbi, to step Merge: 001114
- to stream Ncbi, to step Curation: 001114
- to stream Ncbi, to step Checkpoint: 001114
Links to Exploration step
PMC:1193615Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Severe Acute Respiratory Syndrome-Associated Coronavirus-Specific Protein Enhances Virulence of an Attenuated Murine Coronavirus</title>
<author><name sortKey="Pewe, Lecia" sort="Pewe, Lecia" uniqKey="Pewe L" first="Lecia" last="Pewe">Lecia Pewe</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhou, Haixia" sort="Zhou, Haixia" uniqKey="Zhou H" first="Haixia" last="Zhou">Haixia Zhou</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Netland, Jason" sort="Netland, Jason" uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Tangudu, Chandra" sort="Tangudu, Chandra" uniqKey="Tangudu C" first="Chandra" last="Tangudu">Chandra Tangudu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Olivares, Heidi" sort="Olivares, Heidi" uniqKey="Olivares H" first="Heidi" last="Olivares">Heidi Olivares</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Shi, Lei" sort="Shi, Lei" uniqKey="Shi L" first="Lei" last="Shi">Lei Shi</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Look, Dwight" sort="Look, Dwight" uniqKey="Look D" first="Dwight" last="Look">Dwight Look</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gallagher, Thomas" sort="Gallagher, Thomas" uniqKey="Gallagher T" first="Thomas" last="Gallagher">Thomas Gallagher</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">16103185</idno>
<idno type="pmc">1193615</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1193615</idno>
<idno type="RBID">PMC:1193615</idno>
<idno type="doi">10.1128/JVI.79.17.11335-11342.2005</idno>
<date when="2005">2005</date>
<idno type="wicri:Area/Pmc/Corpus">000696</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000696</idno>
<idno type="wicri:Area/Pmc/Curation">000696</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000696</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001407</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001407</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:16103185</idno>
<idno type="wicri:Area/PubMed/Corpus">002586</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002586</idno>
<idno type="wicri:Area/PubMed/Curation">002586</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002586</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002853</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002853</idno>
<idno type="wicri:Area/Ncbi/Merge">001114</idno>
<idno type="wicri:Area/Ncbi/Curation">001114</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001114</idno>
<idno type="wicri:doubleKey">0022-538X:2005:Pewe L:a:severe:acute</idno>
<idno type="wicri:Area/Main/Merge">004C84</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Severe Acute Respiratory Syndrome-Associated Coronavirus-Specific Protein Enhances Virulence of an Attenuated Murine Coronavirus</title>
<author><name sortKey="Pewe, Lecia" sort="Pewe, Lecia" uniqKey="Pewe L" first="Lecia" last="Pewe">Lecia Pewe</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhou, Haixia" sort="Zhou, Haixia" uniqKey="Zhou H" first="Haixia" last="Zhou">Haixia Zhou</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Netland, Jason" sort="Netland, Jason" uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Tangudu, Chandra" sort="Tangudu, Chandra" uniqKey="Tangudu C" first="Chandra" last="Tangudu">Chandra Tangudu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Olivares, Heidi" sort="Olivares, Heidi" uniqKey="Olivares H" first="Heidi" last="Olivares">Heidi Olivares</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Shi, Lei" sort="Shi, Lei" uniqKey="Shi L" first="Lei" last="Shi">Lei Shi</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Look, Dwight" sort="Look, Dwight" uniqKey="Look D" first="Dwight" last="Look">Dwight Look</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gallagher, Thomas" sort="Gallagher, Thomas" uniqKey="Gallagher T" first="Thomas" last="Gallagher">Thomas Gallagher</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Coronavirus Infections (virology)</term>
<term>Disease Models, Animal</term>
<term>Encephalitis (virology)</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Murine hepatitis virus (genetics)</term>
<term>Murine hepatitis virus (pathogenicity)</term>
<term>Open Reading Frames (genetics)</term>
<term>Reassortant Viruses (pathogenicity)</term>
<term>SARS Virus (chemistry)</term>
<term>SARS Virus (pathogenicity)</term>
<term>SARS Virus (physiology)</term>
<term>Viral Nonstructural Proteins (genetics)</term>
<term>Viral Nonstructural Proteins (metabolism)</term>
<term>Virulence</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cadres ouverts de lecture (génétique)</term>
<term>Encéphalite (virologie)</term>
<term>Humains</term>
<term>Infections à coronavirus (virologie)</term>
<term>Lignée cellulaire</term>
<term>Modèles animaux de maladie humaine</term>
<term>Protéines virales non structurales (génétique)</term>
<term>Protéines virales non structurales (métabolisme)</term>
<term>Réplication virale</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Virulence</term>
<term>Virus de l'hépatite murine (génétique)</term>
<term>Virus de l'hépatite murine (pathogénicité)</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (pathogénicité)</term>
<term>Virus du SRAS (physiologie)</term>
<term>Virus recombinants (pathogénicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Nonstructural Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Murine hepatitis virus</term>
<term>Open Reading Frames</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cadres ouverts de lecture</term>
<term>Protéines virales non structurales</term>
<term>Virus de l'hépatite murine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Nonstructural Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines virales non structurales</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Murine hepatitis virus</term>
<term>Reassortant Viruses</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de l'hépatite murine</term>
<term>Virus du SRAS</term>
<term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Encéphalite</term>
<term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Encephalitis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Disease Models, Animal</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Virulence</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Modèles animaux de maladie humaine</term>
<term>Réplication virale</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Virulence</term>
<term>Virus du SRAS</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Most animal species that can be infected with the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) do not reproducibly develop clinical disease, hindering studies of pathogenesis. To develop an alternative system for the study of SARS-CoV, we introduced individual SARS-CoV genes (open reading frames [ORFs]) into the genome of an attenuated murine coronavirus. One protein, the product of SARS-CoV ORF6, converted a sublethal infection to a uniformly lethal encephalitis and enhanced virus growth in tissue culture cells, indicating that SARS-CoV proteins function in the context of a heterologous coronavirus infection. Furthermore, these results suggest that the attenuated murine coronavirus lacks a virulence gene residing in SARS-CoV. Recombinant murine coronaviruses cause a reproducible and well-characterized clinical disease, offer virtually no risk to laboratory personnel, and should be useful for elucidating the role of SARS-CoV nonstructural proteins in viral replication and pathogenesis.</p>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004C84 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004C84 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Merge |type= RBID |clé= PMC:1193615 |texte= A Severe Acute Respiratory Syndrome-Associated Coronavirus-Specific Protein Enhances Virulence of an Attenuated Murine Coronavirus }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:16103185" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |