Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells
Identifieur interne : 005064 ( Main/Exploration ); précédent : 005063; suivant : 005065Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells
Auteurs : Ling Yi ; Zhengquan Li ; Kehu Yuan ; Xiuxia Qu ; Jian Chen ; Guangwen Wang ; Hong Zhang ; Hongpeng Luo ; Lili Zhu ; Pengfei Jiang ; Lirong Chen ; Yan Shen ; Min Luo ; Guoying Zuo ; Jianhe Hu ; Deliang Duan ; Yuchun Nie ; Xuanling Shi ; Wei Wang ; Yang Han ; Taisheng Li ; Yuqing Liu ; Mingxiao Ding ; Hongkui Deng ; Xiaojie XuSource :
- Journal of Virology [ 0022-538X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Antiviraux (), Antiviraux (métabolisme), Antiviraux (pharmacologie), Cellules Vero, Chine, Chromatographie d'affinité, Flavonoïdes (pharmacologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Humains, Lignée cellulaire, Luciferases (génétique), Luciferases (métabolisme), Lutéoline, Plantes médicinales (), Plantes médicinales (métabolisme), Protéines de l'enveloppe virale (métabolisme), Spectrométrie de masse, Tanins hydrolysables (), Tanins hydrolysables (pharmacologie), Tests de sensibilité microbienne, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (métabolisme), Virus du SRAS (), Virus du SRAS (pathogénicité).
- MESH :
- génétique : Luciferases, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- métabolisme : Antiviraux, Glycoprotéines membranaires, Luciferases, Plantes médicinales, Protéines de l'enveloppe virale, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- pathogénicité : Virus du SRAS.
- pharmacologie : Antiviraux, Flavonoïdes, Tanins hydrolysables.
- Animaux, Antiviraux, Cellules Vero, Chine, Chromatographie d'affinité, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Lutéoline, Plantes médicinales, Spectrométrie de masse, Tanins hydrolysables, Tests de sensibilité microbienne, Virus du SRAS.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (chemistry), Antiviral Agents (metabolism), Antiviral Agents (pharmacology), Cell Line, China, Chlorocebus aethiops, Chromatography, Affinity, Flavonoids (pharmacology), HIV-1 (genetics), HIV-1 (metabolism), Humans, Hydrolyzable Tannins (chemistry), Hydrolyzable Tannins (pharmacology), Luciferases (genetics), Luciferases (metabolism), Luteolin, Mass Spectrometry, Membrane Glycoproteins (metabolism), Microbial Sensitivity Tests, Plants, Medicinal (chemistry), Plants, Medicinal (metabolism), SARS Virus (drug effects), SARS Virus (pathogenicity), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Antiviral Agents, Hydrolyzable Tannins.
- chemical , genetics : Luciferases.
- chemical , metabolism : Antiviral Agents, Luciferases, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , pharmacology : Antiviral Agents, Flavonoids, Hydrolyzable Tannins.
- geographic : China, Luteolin, Spike Glycoprotein, Coronavirus.
- chemistry : Plants, Medicinal.
- drug effects : SARS Virus.
- genetics : HIV-1.
- metabolism : HIV-1, Plants, Medicinal.
- pathogenicity : SARS Virus.
- Animals, Cell Line, Chlorocebus aethiops, Chromatography, Affinity, Humans, Mass Spectrometry, Microbial Sensitivity Tests, Vero Cells.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-
Url:
DOI: 10.1128/JVI.78.20.11334-11339.2004
PubMed: 15452254
PubMed Central: 521800
Affiliations:
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Le document en format XML
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<author><name sortKey="Li, Taisheng" sort="Li, Taisheng" uniqKey="Li T" first="Taisheng" last="Li">Taisheng Li</name>
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<author><name sortKey="Liu, Yuqing" sort="Liu, Yuqing" uniqKey="Liu Y" first="Yuqing" last="Liu">Yuqing Liu</name>
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<author><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name>
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<series><title level="j">Journal of Virology</title>
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<term>Antiviral Agents (chemistry)</term>
<term>Antiviral Agents (metabolism)</term>
<term>Antiviral Agents (pharmacology)</term>
<term>Cell Line</term>
<term>China</term>
<term>Chlorocebus aethiops</term>
<term>Chromatography, Affinity</term>
<term>Flavonoids (pharmacology)</term>
<term>HIV-1 (genetics)</term>
<term>HIV-1 (metabolism)</term>
<term>Humans</term>
<term>Hydrolyzable Tannins (chemistry)</term>
<term>Hydrolyzable Tannins (pharmacology)</term>
<term>Luciferases (genetics)</term>
<term>Luciferases (metabolism)</term>
<term>Luteolin</term>
<term>Mass Spectrometry</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Microbial Sensitivity Tests</term>
<term>Plants, Medicinal (chemistry)</term>
<term>Plants, Medicinal (metabolism)</term>
<term>SARS Virus (drug effects)</term>
<term>SARS Virus (pathogenicity)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Antiviraux ()</term>
<term>Antiviraux (métabolisme)</term>
<term>Antiviraux (pharmacologie)</term>
<term>Cellules Vero</term>
<term>Chine</term>
<term>Chromatographie d'affinité</term>
<term>Flavonoïdes (pharmacologie)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Luciferases (génétique)</term>
<term>Luciferases (métabolisme)</term>
<term>Lutéoline</term>
<term>Plantes médicinales ()</term>
<term>Plantes médicinales (métabolisme)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Spectrométrie de masse</term>
<term>Tanins hydrolysables ()</term>
<term>Tanins hydrolysables (pharmacologie)</term>
<term>Tests de sensibilité microbienne</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (métabolisme)</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (pathogénicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antiviral Agents</term>
<term>Hydrolyzable Tannins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luciferases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antiviral Agents</term>
<term>Luciferases</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term>
<term>Flavonoids</term>
<term>Hydrolyzable Tannins</term>
</keywords>
<keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term>
<term>Luteolin</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plants, Medicinal</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Luciferases</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>HIV-1</term>
<term>Plants, Medicinal</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antiviraux</term>
<term>Glycoprotéines membranaires</term>
<term>Luciferases</term>
<term>Plantes médicinales</term>
<term>Protéines de l'enveloppe virale</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term>
<term>Flavonoïdes</term>
<term>Tanins hydrolysables</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Chromatography, Affinity</term>
<term>Humans</term>
<term>Mass Spectrometry</term>
<term>Microbial Sensitivity Tests</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Antiviraux</term>
<term>Cellules Vero</term>
<term>Chine</term>
<term>Chromatographie d'affinité</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Lutéoline</term>
<term>Plantes médicinales</term>
<term>Spectrométrie de masse</term>
<term>Tanins hydrolysables</term>
<term>Tests de sensibilité microbienne</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-<italic>O</italic>
-galloyl-β-<sc>d</sc>
-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 μM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name>
<name sortKey="Chen, Lirong" sort="Chen, Lirong" uniqKey="Chen L" first="Lirong" last="Chen">Lirong Chen</name>
<name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name>
<name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name>
<name sortKey="Duan, Deliang" sort="Duan, Deliang" uniqKey="Duan D" first="Deliang" last="Duan">Deliang Duan</name>
<name sortKey="Han, Yang" sort="Han, Yang" uniqKey="Han Y" first="Yang" last="Han">Yang Han</name>
<name sortKey="Hu, Jianhe" sort="Hu, Jianhe" uniqKey="Hu J" first="Jianhe" last="Hu">Jianhe Hu</name>
<name sortKey="Jiang, Pengfei" sort="Jiang, Pengfei" uniqKey="Jiang P" first="Pengfei" last="Jiang">Pengfei Jiang</name>
<name sortKey="Li, Taisheng" sort="Li, Taisheng" uniqKey="Li T" first="Taisheng" last="Li">Taisheng Li</name>
<name sortKey="Li, Zhengquan" sort="Li, Zhengquan" uniqKey="Li Z" first="Zhengquan" last="Li">Zhengquan Li</name>
<name sortKey="Liu, Yuqing" sort="Liu, Yuqing" uniqKey="Liu Y" first="Yuqing" last="Liu">Yuqing Liu</name>
<name sortKey="Luo, Hongpeng" sort="Luo, Hongpeng" uniqKey="Luo H" first="Hongpeng" last="Luo">Hongpeng Luo</name>
<name sortKey="Luo, Min" sort="Luo, Min" uniqKey="Luo M" first="Min" last="Luo">Min Luo</name>
<name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name>
<name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name>
<name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name>
<name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name>
<name sortKey="Wang, Guangwen" sort="Wang, Guangwen" uniqKey="Wang G" first="Guangwen" last="Wang">Guangwen Wang</name>
<name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
<name sortKey="Xu, Xiaojie" sort="Xu, Xiaojie" uniqKey="Xu X" first="Xiaojie" last="Xu">Xiaojie Xu</name>
<name sortKey="Yi, Ling" sort="Yi, Ling" uniqKey="Yi L" first="Ling" last="Yi">Ling Yi</name>
<name sortKey="Yuan, Kehu" sort="Yuan, Kehu" uniqKey="Yuan K" first="Kehu" last="Yuan">Kehu Yuan</name>
<name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name>
<name sortKey="Zhu, Lili" sort="Zhu, Lili" uniqKey="Zhu L" first="Lili" last="Zhu">Lili Zhu</name>
<name sortKey="Zuo, Guoying" sort="Zuo, Guoying" uniqKey="Zuo G" first="Guoying" last="Zuo">Guoying Zuo</name>
</noCountry>
</tree>
</affiliations>
</record>
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