Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells
Identifieur interne : 000685 ( Pmc/Curation ); précédent : 000684; suivant : 000686Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells
Auteurs : Ling Yi ; Zhengquan Li ; Kehu Yuan ; Xiuxia Qu ; Jian Chen ; Guangwen Wang ; Hong Zhang ; Hongpeng Luo ; Lili Zhu ; Pengfei Jiang ; Lirong Chen ; Yan Shen ; Min Luo ; Guoying Zuo ; Jianhe Hu ; Deliang Duan ; Yuchun Nie ; Xuanling Shi ; Wei Wang ; Yang Han ; Taisheng Li ; Yuqing Liu ; Mingxiao Ding ; Hongkui Deng ; Xiaojie XuSource :
- Journal of Virology [ 0022-538X ] ; 2004.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-
Url:
DOI: 10.1128/JVI.78.20.11334-11339.2004
PubMed: 15452254
PubMed Central: 521800
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<series><title level="j">Journal of Virology</title>
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<front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-<italic>O</italic>
-galloyl-β-<sc>d</sc>
-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 μM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.</p>
</div>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id>
<journal-id journal-id-type="publisher-id">jvi</journal-id>
<journal-title>Journal of Virology</journal-title>
<issn pub-type="ppub">0022-538X</issn>
<issn pub-type="epub">1098-5514</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
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<article-id pub-id-type="pmc">521800</article-id>
<article-id pub-id-type="publisher-id">0206-04</article-id>
<article-id pub-id-type="doi">10.1128/JVI.78.20.11334-11339.2004</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Vaccines and Antiviral Agents</subject>
</subj-group>
</article-categories>
<title-group><article-title>Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Yi</surname>
<given-names>Ling</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="fn" rid="fn1">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Zhengquan</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="fn" rid="fn1">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yuan</surname>
<given-names>Kehu</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="fn" rid="fn1">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Qu</surname>
<given-names>Xiuxia</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chen</surname>
<given-names>Jian</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Guangwen</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Hong</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Luo</surname>
<given-names>Hongpeng</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhu</surname>
<given-names>Lili</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jiang</surname>
<given-names>Pengfei</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chen</surname>
<given-names>Lirong</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Shen</surname>
<given-names>Yan</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Luo</surname>
<given-names>Min</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zuo</surname>
<given-names>Guoying</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hu</surname>
<given-names>Jianhe</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Duan</surname>
<given-names>Deliang</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Nie</surname>
<given-names>Yuchun</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Shi</surname>
<given-names>Xuanling</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Wei</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Han</surname>
<given-names>Yang</given-names>
</name>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Taisheng</given-names>
</name>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Yuqing</given-names>
</name>
<xref ref-type="aff" rid="aff1">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ding</surname>
<given-names>Mingxiao</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Deng</surname>
<given-names>Hongkui</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Xu</surname>
<given-names>Xiaojie</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
</contrib-group>
<aff id="aff1">Department of Cell Biology and Genetics, College of Life Sciences,<label>1</label>
College of Chemistry and Molecular Engineering, Peking University,<label>2</label>
Department of Infectious Disease, PUMC Hospital, CAMS and PUMC, Beijing,<label>3</label>
Centre for the Study of Liver Disease and Department of Surgery, The University of Hong Kong, Pokfulam, Hong Kong, Peoples Republic of China<label>4</label>
</aff>
<author-notes><fn id="cor1"><label>*</label>
<p>Corresponding author. Mailing address for H. Deng: Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, Peoples Republic of China. Phone: 8610-6275-6474. Fax: 8610-6275-6474. E-mail: <email>hongkui_deng@pku.edu.cn</email>
. Mailing address for X. Xu: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, Peoples Republic of China. Phone: 8610-6275-7456. Fax: 8610-6275-1708. E-mail: <email>xiaojxu@chem.pku.edu.cn</email>
.</p>
</fn>
<fn id="fn1"><label>†</label>
<p>L.Y., Z.L., and K.Y. contributed equally to this study.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>10</month>
<year>2004</year>
</pub-date>
<volume>78</volume>
<issue>20</issue>
<fpage>11334</fpage>
<lpage>11339</lpage>
<history><date date-type="received"><day>30</day>
<month>1</month>
<year>2004</year>
</date>
<date date-type="accepted"><day>14</day>
<month>6</month>
<year>2004</year>
</date>
</history>
<copyright-statement>Copyright © 2004, American Society for Microbiology</copyright-statement>
<copyright-year>2004</copyright-year>
<abstract><p>Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-<italic>O</italic>
-galloyl-β-<sc>d</sc>
-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 μM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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