Inactivation of SARS coronavirus by means of povidone-lodine, physical conditions and chemical reagents
Identifieur interne : 004440 ( Main/Exploration ); précédent : 004439; suivant : 004441Inactivation of SARS coronavirus by means of povidone-lodine, physical conditions and chemical reagents
Auteurs : Hiroaki Kariwa [Japon] ; Nobuhiro Fujii [Japon] ; Ikuo Takashima [Japon]Source :
- Dermatology : (Basel) [ 1018-8665 ] ; 2006.
Descripteurs français
- KwdFr :
- Acétone (pharmacologie), Animaux, Anti-infectieux locaux (pharmacologie), Antiviraux (pharmacologie), Cellules Vero, Formaldéhyde (pharmacologie), Glutaraldéhyde (pharmacologie), Inactivation virale, Indicateurs et réactifs (pharmacologie), Méthanol (pharmacologie), Povidone iodée (pharmacologie), Rayons ultraviolets, Température élevée, Virus du SRAS (), Virus du SRAS (effets des radiations), Éthanol (pharmacologie).
- MESH :
- effets des radiations : Virus du SRAS.
- pharmacologie : Acétone, Anti-infectieux locaux, Antiviraux, Formaldéhyde, Glutaraldéhyde, Indicateurs et réactifs, Méthanol, Povidone iodée, Éthanol.
- Pascal (Inist)
- Wicri :
- topic : Iode.
English descriptors
- KwdEn :
- Acetone (pharmacology), Animals, Anti-Infective Agents, Local (pharmacology), Antiviral, Antiviral Agents (pharmacology), Biological activity, Chlorocebus aethiops, Coronavirus, Ethanol (pharmacology), Formaldehyde (pharmacology), Glutaral (pharmacology), Hot Temperature, In vitro, Indicators and Reagents (pharmacology), Iodine, Methanol (pharmacology), Povidone, Povidone-Iodine (pharmacology), SARS Virus (drug effects), SARS Virus (radiation effects), Severe acute respiratory syndrome, Ultraviolet Rays, Vero Cells, Virus Inactivation.
- MESH :
- chemical , pharmacology : Acetone, Anti-Infective Agents, Local, Antiviral Agents, Ethanol, Formaldehyde, Glutaral, Indicators and Reagents, Methanol, Povidone-Iodine.
- drug effects : SARS Virus.
- radiation effects : SARS Virus.
- Animals, Chlorocebus aethiops, Hot Temperature, Ultraviolet Rays, Vero Cells, Virus Inactivation.
Abstract
The efficacy of several povidone-iodine (PVP-I) products, a number of other chemical agents and various physical conditions were evaluated for their ability to inactivate the severe acute respiratory syndrome coronavirus (SARS-CoV). Treatment of SARS-CoV with PVP-I products for 2 min reduced the virus infectivity from 1.17 x 106 TCID50/ml to below the detectable level. The efficacy of 70% ethanol was equivalent to that of PVP-I products. Fixation of SARS-CoV-infected Vero E6 cells with a fixative including formalin, glutaraldehyde, methanol and acetone for 5 min or longer eliminated all infectivity. Heating the virus at 56°C for 60 min or longer reduced the infectivity of the virus from 2.6 x 107 to undetectable levels. Irradiation with ultraviolet light at 134 μW/cm2 for 15 min reduced the infectivity from 3.8 x 107 to 180 TCID50/ml; however, prolonged irradiation (60 min) failed to eliminate the remaining virus, leaving 18.8 TCID50/ml.
Affiliations:
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Le document en format XML
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<term>Animals</term>
<term>Anti-Infective Agents, Local (pharmacology)</term>
<term>Antiviral</term>
<term>Antiviral Agents (pharmacology)</term>
<term>Biological activity</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus</term>
<term>Ethanol (pharmacology)</term>
<term>Formaldehyde (pharmacology)</term>
<term>Glutaral (pharmacology)</term>
<term>Hot Temperature</term>
<term>In vitro</term>
<term>Indicators and Reagents (pharmacology)</term>
<term>Iodine</term>
<term>Methanol (pharmacology)</term>
<term>Povidone</term>
<term>Povidone-Iodine (pharmacology)</term>
<term>SARS Virus (drug effects)</term>
<term>SARS Virus (radiation effects)</term>
<term>Severe acute respiratory syndrome</term>
<term>Ultraviolet Rays</term>
<term>Vero Cells</term>
<term>Virus Inactivation</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acétone (pharmacologie)</term>
<term>Animaux</term>
<term>Anti-infectieux locaux (pharmacologie)</term>
<term>Antiviraux (pharmacologie)</term>
<term>Cellules Vero</term>
<term>Formaldéhyde (pharmacologie)</term>
<term>Glutaraldéhyde (pharmacologie)</term>
<term>Inactivation virale</term>
<term>Indicateurs et réactifs (pharmacologie)</term>
<term>Méthanol (pharmacologie)</term>
<term>Povidone iodée (pharmacologie)</term>
<term>Rayons ultraviolets</term>
<term>Température élevée</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (effets des radiations)</term>
<term>Éthanol (pharmacologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Acetone</term>
<term>Anti-Infective Agents, Local</term>
<term>Antiviral Agents</term>
<term>Ethanol</term>
<term>Formaldehyde</term>
<term>Glutaral</term>
<term>Indicators and Reagents</term>
<term>Methanol</term>
<term>Povidone-Iodine</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acétone</term>
<term>Anti-infectieux locaux</term>
<term>Antiviraux</term>
<term>Formaldéhyde</term>
<term>Glutaraldéhyde</term>
<term>Indicateurs et réactifs</term>
<term>Méthanol</term>
<term>Povidone iodée</term>
<term>Éthanol</term>
</keywords>
<keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Hot Temperature</term>
<term>Ultraviolet Rays</term>
<term>Vero Cells</term>
<term>Virus Inactivation</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Inactivation virale</term>
<term>Rayons ultraviolets</term>
<term>Syndrome respiratoire aigu sévère</term>
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<term>Coronavirus</term>
<term>Iode</term>
<term>In vitro</term>
<term>Activité biologique</term>
<term>Antiviral</term>
<term>Température élevée</term>
<term>Virus du SRAS</term>
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<front><div type="abstract" xml:lang="en">The efficacy of several povidone-iodine (PVP-I) products, a number of other chemical agents and various physical conditions were evaluated for their ability to inactivate the severe acute respiratory syndrome coronavirus (SARS-CoV). Treatment of SARS-CoV with PVP-I products for 2 min reduced the virus infectivity from 1.17 x 10<sup>6</sup>
TCID<sub>50</sub>
/ml to below the detectable level. The efficacy of 70% ethanol was equivalent to that of PVP-I products. Fixation of SARS-CoV-infected Vero E6 cells with a fixative including formalin, glutaraldehyde, methanol and acetone for 5 min or longer eliminated all infectivity. Heating the virus at 56°C for 60 min or longer reduced the infectivity of the virus from 2.6 x 10<sup>7</sup>
to undetectable levels. Irradiation with ultraviolet light at 134 μW/cm<sup>2</sup>
for 15 min reduced the infectivity from 3.8 x 10<sup>7</sup>
to 180 TCID<sub>50</sub>
/ml; however, prolonged irradiation (60 min) failed to eliminate the remaining virus, leaving 18.8 TCID<sub>50</sub>
/ml.</div>
</front>
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<tree><country name="Japon"><noRegion><name sortKey="Kariwa, Hiroaki" sort="Kariwa, Hiroaki" uniqKey="Kariwa H" first="Hiroaki" last="Kariwa">Hiroaki Kariwa</name>
</noRegion>
<name sortKey="Fujii, Nobuhiro" sort="Fujii, Nobuhiro" uniqKey="Fujii N" first="Nobuhiro" last="Fujii">Nobuhiro Fujii</name>
<name sortKey="Takashima, Ikuo" sort="Takashima, Ikuo" uniqKey="Takashima I" first="Ikuo" last="Takashima">Ikuo Takashima</name>
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