Synthetic peptides as receptors in affinity sensors : a feasibility study
Identifieur interne : 006602 ( Main/Exploration ); précédent : 006601; suivant : 006603Synthetic peptides as receptors in affinity sensors : a feasibility study
Auteurs : D. J. Van Den Heuvel [Pays-Bas] ; R. P. H. Kooyman [Pays-Bas] ; J. W. Drijfhout ; G. W. WellingSource :
- Analytical biochemistry [ 0003-2697 ] ; 1993.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Protéines de l'enveloppe virale.
- immunologie : Peptides, Protéines de l'enveloppe virale.
- Pascal (Inist)
- Anticorps monoclonaux, Or, Peptide, Peptides, Produit synthétique, Herpesvirus hominis, Protéine, Sites de fixation, Séquence aminoacide, Modification chimique, Immobilisation, Or, Couche monomoléculaire, Interaction moléculaire, Anticorps monoclonal, Capacité fixation, Application, Immunodétecteur, Techniques de biocapteur, Thiols, Études de faisabilité.
- Wicri :
- topic : Or.
English descriptors
- KwdEn :
- Aminoacid sequence, Antibodies, Monoclonal, Application, Binding Sites, Binding capacity, Biosensing Techniques, Chemical modification, Feasibility Studies, Gold, Gold (chemistry), Herpesvirus hominis, Immobilization, Immunosensor, Molecular interaction, Monoclonal antibody, Monolayer, Peptides, Peptides (chemistry), Peptides (immunology), Proteins, Sulfhydryl Compounds (chemistry), Synthetic product, Viral Envelope Proteins (analysis), Viral Envelope Proteins (immunology).
- MESH :
- chemical , analysis : Viral Envelope Proteins.
- chemical , chemistry : Gold, Peptides, Sulfhydryl Compounds.
- chemical , immunology : Peptides, Viral Envelope Proteins.
- chemical : Antibodies, Monoclonal.
- Binding Sites, Biosensing Techniques, Feasibility Studies.
Abstract
A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage
Affiliations:
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Le document en format XML
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J." last="Van Den Heuvel">D. J. Van Den Heuvel</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Twente, MESA-inst., dep. applied physics</s1><s2>7500 AE Enschede</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>7500 AE Enschede</wicri:noRegion></affiliation></author><author><name sortKey="Kooyman, R P H" sort="Kooyman, R P H" uniqKey="Kooyman R" first="R. P. H." last="Kooyman">R. P. H. Kooyman</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Twente, MESA-inst., dep. applied physics</s1><s2>7500 AE Enschede</s2><s3>NLD</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>7500 AE Enschede</wicri:noRegion></affiliation></author><author><name sortKey="Drijfhout, J W" sort="Drijfhout, J W" uniqKey="Drijfhout J" first="J. W." last="Drijfhout">J. W. Drijfhout</name></author><author><name sortKey="Welling, G W" sort="Welling, G W" uniqKey="Welling G" first="G. W." last="Welling">G. W. Welling</name></author></analytic><series><title level="j" type="main">Analytical biochemistry</title><title level="j" type="abbreviated">Anal. biochem.</title><idno type="ISSN">0003-2697</idno><imprint><date when="1993">1993</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Analytical biochemistry</title><title level="j" type="abbreviated">Anal. biochem.</title><idno type="ISSN">0003-2697</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aminoacid sequence</term><term>Antibodies, Monoclonal</term><term>Application</term><term>Binding Sites</term><term>Binding capacity</term><term>Biosensing Techniques</term><term>Chemical modification</term><term>Feasibility Studies</term><term>Gold</term><term>Gold (chemistry)</term><term>Herpesvirus hominis</term><term>Immobilization</term><term>Immunosensor</term><term>Molecular interaction</term><term>Monoclonal antibody</term><term>Monolayer</term><term>Peptides</term><term>Peptides (chemistry)</term><term>Peptides (immunology)</term><term>Proteins</term><term>Sulfhydryl Compounds (chemistry)</term><term>Synthetic product</term><term>Viral Envelope Proteins (analysis)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Or ()</term><term>Peptides ()</term><term>Peptides (immunologie)</term><term>Protéines de l'enveloppe virale (analyse)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Sites de fixation</term><term>Techniques de biocapteur</term><term>Thiols ()</term><term>Études de faisabilité</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Gold</term><term>Peptides</term><term>Sulfhydryl Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Peptides</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Peptides</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term><term>Biosensing Techniques</term><term>Feasibility Studies</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Or</term><term>Peptide</term><term>Peptides</term><term>Produit synthétique</term><term>Herpesvirus hominis</term><term>Protéine</term><term>Sites de fixation</term><term>Séquence aminoacide</term><term>Modification chimique</term><term>Immobilisation</term><term>Or</term><term>Couche monomoléculaire</term><term>Interaction moléculaire</term><term>Anticorps monoclonal</term><term>Capacité fixation</term><term>Application</term><term>Immunodétecteur</term><term>Techniques de biocapteur</term><term>Thiols</term><term>Études de faisabilité</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Or</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><noCountry><name sortKey="Drijfhout, J W" sort="Drijfhout, J W" uniqKey="Drijfhout J" first="J. W." last="Drijfhout">J. W. Drijfhout</name><name sortKey="Welling, G W" sort="Welling, G W" uniqKey="Welling G" first="G. W." last="Welling">G. W. Welling</name></noCountry><country name="Pays-Bas"><noRegion><name sortKey="Van Den Heuvel, D J" sort="Van Den Heuvel, D J" uniqKey="Van Den Heuvel D" first="D. J." last="Van Den Heuvel">D. J. Van Den Heuvel</name></noRegion><name sortKey="Kooyman, R P H" sort="Kooyman, R P H" uniqKey="Kooyman R" first="R. P. H." last="Kooyman">R. P. H. Kooyman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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