Synthetic peptides as receptors in affinity sensors : a feasibility study
Identifieur interne : 000990 ( PascalFrancis/Curation ); précédent : 000989; suivant : 000991Synthetic peptides as receptors in affinity sensors : a feasibility study
Auteurs : D. J. Van Den Heuvel [Pays-Bas] ; R. P. H. Kooyman [Pays-Bas] ; J. W. Drijfhout ; G. W. WellingSource :
- Analytical biochemistry [ 0003-2697 ] ; 1993.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Or.
English descriptors
- KwdEn :
Abstract
A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage
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<author><name sortKey="Van Den Heuvel, D J" sort="Van Den Heuvel, D J" uniqKey="Van Den Heuvel D" first="D. J." last="Van Den Heuvel">D. J. Van Den Heuvel</name>
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<term>Gold</term>
<term>Herpesvirus hominis</term>
<term>Immobilization</term>
<term>Immunosensor</term>
<term>Molecular interaction</term>
<term>Monoclonal antibody</term>
<term>Monolayer</term>
<term>Peptides</term>
<term>Proteins</term>
<term>Synthetic product</term>
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<term>Protéine</term>
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<term>Or</term>
<term>Couche monomoléculaire</term>
<term>Interaction moléculaire</term>
<term>Anticorps monoclonal</term>
<term>Capacité fixation</term>
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<front><div type="abstract" xml:lang="en">A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage</div>
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<fC01 i1="01" l="ENG"><s0>A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage</s0>
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<s5>01</s5>
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<s5>01</s5>
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<s5>05</s5>
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<s5>05</s5>
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<s5>09</s5>
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<s5>13</s5>
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<s5>13</s5>
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<s5>13</s5>
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<s5>17</s5>
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<s5>17</s5>
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<s5>17</s5>
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<s5>21</s5>
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<s5>21</s5>
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<s5>21</s5>
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<s5>43</s5>
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<s5>43</s5>
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<s5>43</s5>
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<s5>46</s5>
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<s5>46</s5>
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<s5>46</s5>
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<s5>46</s5>
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<s5>49</s5>
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<s5>49</s5>
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<s5>49</s5>
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<s5>52</s5>
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<s5>52</s5>
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<s5>52</s5>
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<s5>55</s5>
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<s5>55</s5>
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<fC03 i1="11" i2="X" l="SPA"><s0>Anticuerpo monoclonal</s0>
<s5>55</s5>
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<s5>58</s5>
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<fC03 i1="12" i2="X" l="ENG"><s0>Binding capacity</s0>
<s5>58</s5>
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<fC03 i1="12" i2="X" l="SPA"><s0>Capacidad de fijación</s0>
<s5>58</s5>
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<s5>61</s5>
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<fC03 i1="13" i2="X" l="ENG"><s0>Application</s0>
<s5>61</s5>
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<fC03 i1="13" i2="X" l="GER"><s0>Anwendung</s0>
<s5>61</s5>
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<s5>61</s5>
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<s4>CD</s4>
<s5>97</s5>
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<s4>CD</s4>
<s5>97</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Alphaherpesvirinae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Alphaherpesvirinae</s0>
<s2>NW</s2>
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<fC07 i1="01" i2="X" l="SPA"><s0>Alphaherpesvirinae</s0>
<s2>NW</s2>
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<fC07 i1="02" i2="X" l="FRE"><s0>Herpesviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Herpesviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Herpesviridae</s0>
<s2>NW</s2>
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<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
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<s2>NW</s2>
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<s5>75</s5>
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<s5>75</s5>
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<fC07 i1="04" i2="X" l="SPA"><s0>Biodetector</s0>
<s5>75</s5>
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<fN21><s1>080</s1>
</fN21>
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