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Synthetic peptides as receptors in affinity sensors : a feasibility study

Identifieur interne : 000994 ( PascalFrancis/Corpus ); précédent : 000993

Synthetic peptides as receptors in affinity sensors : a feasibility study

Auteurs : D. J. Van Den Heuvel ; R. P. H. Kooyman ; J. W. Drijfhout ; G. W. Welling

Source :

RBID : Pascal:94-0159445

Descripteurs français

English descriptors

Abstract

A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 0003-2697
A02 01      @0 ANBCA2
A03   1    @0 Anal. biochem.
A05       @2 215
A06       @2 2
A08 01  1  ENG  @1 Synthetic peptides as receptors in affinity sensors : a feasibility study
A11 01  1    @1 VAN DEN HEUVEL (D. J.)
A11 02  1    @1 KOOYMAN (R. P. H.)
A11 03  1    @1 DRIJFHOUT (J. W.)
A11 04  1    @1 WELLING (G. W.)
A14 01      @1 Univ. Twente, MESA-inst., dep. applied physics @2 7500 AE Enschede @3 NLD @Z 1 aut. @Z 2 aut.
A20       @1 223-230
A21       @1 1993
A23 01      @0 ENG
A43 01      @1 INIST @2 2981 @5 354000023999220090
A44       @0 0000
A45       @0 30 ref.
A47 01  1    @0 94-0159445
A60       @1 P
A61       @0 A
A64 01  1    @0 Analytical biochemistry
A66 01      @0 USA
C01 01    ENG  @0 A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage
C02 01  X    @0 002A31C09B
C02 02  X    @0 215
C03 01  X  FRE  @0 Peptide @5 01
C03 01  X  ENG  @0 Peptides @5 01
C03 01  X  SPA  @0 Péptido @5 01
C03 02  X  FRE  @0 Produit synthétique @5 05
C03 02  X  ENG  @0 Synthetic product @5 05
C03 02  X  SPA  @0 Producto sintético @5 05
C03 03  X  FRE  @0 Herpesvirus hominis @2 NW @5 09
C03 03  X  ENG  @0 Herpesvirus hominis @2 NW @5 09
C03 03  X  SPA  @0 Herpesvirus hominis @2 NW @5 09
C03 04  X  FRE  @0 Protéine @5 13
C03 04  X  ENG  @0 Proteins @5 13
C03 04  X  SPA  @0 Proteína @5 13
C03 05  X  FRE  @0 Séquence aminoacide @5 17
C03 05  X  ENG  @0 Aminoacid sequence @5 17
C03 05  X  SPA  @0 Secuencia aminoácido @5 17
C03 06  X  FRE  @0 Modification chimique @5 21
C03 06  X  ENG  @0 Chemical modification @5 21
C03 06  X  SPA  @0 Modificación química @5 21
C03 07  X  FRE  @0 Immobilisation @5 43
C03 07  X  ENG  @0 Immobilization @5 43
C03 07  X  SPA  @0 Inmovilización @5 43
C03 08  X  FRE  @0 Or @2 NC @5 46
C03 08  X  ENG  @0 Gold @2 NC @5 46
C03 08  X  GER  @0 Gold @2 NC @5 46
C03 08  X  SPA  @0 Oro @2 NC @5 46
C03 09  X  FRE  @0 Couche monomoléculaire @5 49
C03 09  X  ENG  @0 Monolayer @5 49
C03 09  X  SPA  @0 Capa monomolecular @5 49
C03 10  X  FRE  @0 Interaction moléculaire @5 52
C03 10  X  ENG  @0 Molecular interaction @5 52
C03 10  X  SPA  @0 Interacción molecular @5 52
C03 11  X  FRE  @0 Anticorps monoclonal @5 55
C03 11  X  ENG  @0 Monoclonal antibody @5 55
C03 11  X  SPA  @0 Anticuerpo monoclonal @5 55
C03 12  X  FRE  @0 Capacité fixation @5 58
C03 12  X  ENG  @0 Binding capacity @5 58
C03 12  X  SPA  @0 Capacidad de fijación @5 58
C03 13  X  FRE  @0 Application @5 61
C03 13  X  ENG  @0 Application @5 61
C03 13  X  GER  @0 Anwendung @5 61
C03 13  X  SPA  @0 Aplicación @5 61
C03 14  X  FRE  @0 Immunodétecteur @4 CD @5 97
C03 14  X  ENG  @0 Immunosensor @4 CD @5 97
C07 01  X  FRE  @0 Alphaherpesvirinae @2 NW
C07 01  X  ENG  @0 Alphaherpesvirinae @2 NW
C07 01  X  SPA  @0 Alphaherpesvirinae @2 NW
C07 02  X  FRE  @0 Herpesviridae @2 NW
C07 02  X  ENG  @0 Herpesviridae @2 NW
C07 02  X  SPA  @0 Herpesviridae @2 NW
C07 03  X  FRE  @0 Virus @2 NW
C07 03  X  ENG  @0 Virus @2 NW
C07 03  X  SPA  @0 Virus @2 NW
C07 04  X  FRE  @0 Biodétecteur @5 75
C07 04  X  ENG  @0 Biosensor @5 75
C07 04  X  SPA  @0 Biodetector @5 75
N21       @1 080

Format Inist (serveur)

NO : PASCAL 94-0159445 INIST
ET : Synthetic peptides as receptors in affinity sensors : a feasibility study
AU : VAN DEN HEUVEL (D. J.); KOOYMAN (R. P. H.); DRIJFHOUT (J. W.); WELLING (G. W.)
AF : Univ. Twente, MESA-inst., dep. applied physics/7500 AE Enschede/Pays-Bas (1 aut., 2 aut.)
DT : Publication en série; Niveau analytique
SO : Analytical biochemistry; ISSN 0003-2697; Coden ANBCA2; Etats-Unis; Da. 1993; Vol. 215; No. 2; Pp. 223-230; Bibl. 30 ref.
LA : Anglais
EA : A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage
CC : 002A31C09B; 215
FD : Peptide; Produit synthétique; Herpesvirus hominis; Protéine; Séquence aminoacide; Modification chimique; Immobilisation; Or; Couche monomoléculaire; Interaction moléculaire; Anticorps monoclonal; Capacité fixation; Application; Immunodétecteur
FG : Alphaherpesvirinae; Herpesviridae; Virus; Biodétecteur
ED : Peptides; Synthetic product; Herpesvirus hominis; Proteins; Aminoacid sequence; Chemical modification; Immobilization; Gold; Monolayer; Molecular interaction; Monoclonal antibody; Binding capacity; Application; Immunosensor
EG : Alphaherpesvirinae; Herpesviridae; Virus; Biosensor
GD : Gold; Anwendung
SD : Péptido; Producto sintético; Herpesvirus hominis; Proteína; Secuencia aminoácido; Modificación química; Inmovilización; Oro; Capa monomolecular; Interacción molecular; Anticuerpo monoclonal; Capacidad de fijación; Aplicación
LO : INIST-2981.354000023999220090
ID : 94-0159445

Links to Exploration step

Pascal:94-0159445

Le document en format XML

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<s5>55</s5>
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<s0>Application</s0>
<s5>61</s5>
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<s0>Anwendung</s0>
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</fC03>
<fC03 i1="13" i2="X" l="SPA">
<s0>Aplicación</s0>
<s5>61</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE">
<s0>Immunodétecteur</s0>
<s4>CD</s4>
<s5>97</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG">
<s0>Immunosensor</s0>
<s4>CD</s4>
<s5>97</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Alphaherpesvirinae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Alphaherpesvirinae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Alphaherpesvirinae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Herpesviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Herpesviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Herpesviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Biodétecteur</s0>
<s5>75</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Biosensor</s0>
<s5>75</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Biodetector</s0>
<s5>75</s5>
</fC07>
<fN21>
<s1>080</s1>
</fN21>
</pA>
</standard>
<server>
<NO>PASCAL 94-0159445 INIST</NO>
<ET>Synthetic peptides as receptors in affinity sensors : a feasibility study</ET>
<AU>VAN DEN HEUVEL (D. J.); KOOYMAN (R. P. H.); DRIJFHOUT (J. W.); WELLING (G. W.)</AU>
<AF>Univ. Twente, MESA-inst., dep. applied physics/7500 AE Enschede/Pays-Bas (1 aut., 2 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Analytical biochemistry; ISSN 0003-2697; Coden ANBCA2; Etats-Unis; Da. 1993; Vol. 215; No. 2; Pp. 223-230; Bibl. 30 ref.</SO>
<LA>Anglais</LA>
<EA>A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage</EA>
<CC>002A31C09B; 215</CC>
<FD>Peptide; Produit synthétique; Herpesvirus hominis; Protéine; Séquence aminoacide; Modification chimique; Immobilisation; Or; Couche monomoléculaire; Interaction moléculaire; Anticorps monoclonal; Capacité fixation; Application; Immunodétecteur</FD>
<FG>Alphaherpesvirinae; Herpesviridae; Virus; Biodétecteur</FG>
<ED>Peptides; Synthetic product; Herpesvirus hominis; Proteins; Aminoacid sequence; Chemical modification; Immobilization; Gold; Monolayer; Molecular interaction; Monoclonal antibody; Binding capacity; Application; Immunosensor</ED>
<EG>Alphaherpesvirinae; Herpesviridae; Virus; Biosensor</EG>
<GD>Gold; Anwendung</GD>
<SD>Péptido; Producto sintético; Herpesvirus hominis; Proteína; Secuencia aminoácido; Modificación química; Inmovilización; Oro; Capa monomolecular; Interacción molecular; Anticuerpo monoclonal; Capacidad de fijación; Aplicación</SD>
<LO>INIST-2981.354000023999220090</LO>
<ID>94-0159445</ID>
</server>
</inist>
</record>

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