Synthetic peptides as receptors in affinity sensors : a feasibility study
Identifieur interne : 006B20 ( Main/Merge ); précédent : 006B19; suivant : 006B21Synthetic peptides as receptors in affinity sensors : a feasibility study
Auteurs : D. J. Van Den Heuvel [Pays-Bas] ; R. P. H. Kooyman [Pays-Bas] ; J. W. Drijfhout ; G. W. WellingSource :
- Analytical biochemistry [ 0003-2697 ] ; 1993.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Or.
English descriptors
- KwdEn :
Abstract
A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage
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Pascal:94-0159445Le document en format XML
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<series><title level="j" type="main">Analytical biochemistry</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aminoacid sequence</term>
<term>Application</term>
<term>Binding capacity</term>
<term>Chemical modification</term>
<term>Gold</term>
<term>Herpesvirus hominis</term>
<term>Immobilization</term>
<term>Immunosensor</term>
<term>Molecular interaction</term>
<term>Monoclonal antibody</term>
<term>Monolayer</term>
<term>Peptides</term>
<term>Proteins</term>
<term>Synthetic product</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Peptide</term>
<term>Produit synthétique</term>
<term>Herpesvirus hominis</term>
<term>Protéine</term>
<term>Séquence aminoacide</term>
<term>Modification chimique</term>
<term>Immobilisation</term>
<term>Or</term>
<term>Couche monomoléculaire</term>
<term>Interaction moléculaire</term>
<term>Anticorps monoclonal</term>
<term>Capacité fixation</term>
<term>Application</term>
<term>Immunodétecteur</term>
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<front><div type="abstract" xml:lang="en">A relatively simple method for immobilizing synthetic peptides as a receptor onto a gold surface using the self-assembling monolayer (SAM) technique has been investigated. A synthetic peptide with an amino acid sequence similar to the 9-21 gD sequence of herpes simplex virus type 1 was modified with an alkylthiol chain and adsorbed in combination with an alkylthiol chain without peptide according to the SAM procedures. The resulting self-assembled receptor layers (SARs) were able to specifically interact with a monoclonal antibody directed against the 9-21 gD peptide. Binding studies monitored with a surface plasmon resonance setup showed that the response to the antibody depended on the composition of the SAR; a maximum response was reached at approximately 3 mol peptide coverage</div>
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<country name="Pays-Bas"><noRegion><name sortKey="Van Den Heuvel, D J" sort="Van Den Heuvel, D J" uniqKey="Van Den Heuvel D" first="D. J." last="Van Den Heuvel">D. J. Van Den Heuvel</name>
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<name sortKey="Kooyman, R P H" sort="Kooyman, R P H" uniqKey="Kooyman R" first="R. P. H." last="Kooyman">R. P. H. Kooyman</name>
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