Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS‐CoV) in SARS patients: implications for pathogenesis and virus transmission pathways
Identifieur interne : 005780 ( Main/Exploration ); précédent : 005779; suivant : 005781Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS‐CoV) in SARS patients: implications for pathogenesis and virus transmission pathways
Auteurs : Yanqing Ding [République populaire de Chine, États-Unis] ; Li He [République populaire de Chine] ; Qingling Zhang [République populaire de Chine] ; Zhongxi Huang [République populaire de Chine] ; Xiaoyan Che [République populaire de Chine] ; Jinlin Hou [République populaire de Chine] ; Huijun Wang [République populaire de Chine] ; Hong Shen [République populaire de Chine] ; Liwen Qiu [République populaire de Chine] ; Zhuguo Li [République populaire de Chine] ; Jian Geng [République populaire de Chine] ; Junjie Cai [République populaire de Chine] ; Huixia Han [République populaire de Chine] ; Xin Li [République populaire de Chine] ; Wei Kang [République populaire de Chine] ; Desheng Weng [République populaire de Chine] ; Ping Liang [République populaire de Chine] ; Shibo Jiang [États-Unis]Source :
- The Journal of Pathology [ 0022-3417 ] ; 2004-06.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Adulte, Adulte d'âge moyen, DNA-directed RNA polymerases (génétique), Femelle, Humains, Hybridation in situ (), Immunohistochimie (), Mâle, Nucléoprotéines (analyse), Poumon (virologie), Protéines virales (analyse), RT-PCR (), Syndrome respiratoire aigu sévère (transmission), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (isolement et purification).
- MESH :
- analyse : Nucléoprotéines, Protéines virales.
- génétique : ARN viral, DNA-directed RNA polymerases.
- isolement et purification : Virus du SRAS.
- virologie : Poumon, Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Femelle, Humains, Hybridation in situ, Immunohistochimie, Mâle, RT-PCR, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Adult, DNA-Directed RNA Polymerases (genetics), Female, Humans, Immunohistochemistry (methods), In Situ Hybridization (methods), Lung (virology), Male, Middle Aged, Nucleoproteins (analysis), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (transmission), Severe Acute Respiratory Syndrome (virology), Viral Proteins (analysis).
- MESH :
- chemical , analysis : Nucleoproteins, Viral Proteins.
- chemical , genetics : DNA-Directed RNA Polymerases, RNA, Viral.
- isolation & purification : SARS Virus.
- methods : Immunohistochemistry, In Situ Hybridization, Reverse Transcriptase Polymerase Chain Reaction.
- transmission : Severe Acute Respiratory Syndrome.
- virology : Lung, Severe Acute Respiratory Syndrome.
- Teeft :
- Ace2, Acidophilic cells, Adrenal gland, Adult, Assay, Autopsy tissues, Bone marrow, Central vein, Cerebral neurones, Control patients, Coronavirus, Cytopathic effect, Ding, Eighth hospital, Epithelial, Epithelial cells, Epithelium, Family members, Fang hospital, Female, Functional receptor, Gastric parietal cells, Gastrointestinal system, Gastrointestinal tract, Gene fragments, Great britain, Guangdong province, Guangzhou, Guangzhou city, Guangzhou institute, Health administration, Humans, Inclusion bodies, Infectious diseases, John wiley sons, Kimball research institute, Light microscope, Little information, Lung tissue, Lung tissues, Lymph node, Male, Medical university, Middle Aged, Natural sciences, Neutral formaldehyde, Novel coronavirus, Organ distribution, Other diseases, Other organs, Other people, Pancreatic acinar cells, Pathol, Pathological changes, Pathological society, Polymerase, Polymerase gene expression, Polymerase gene fragment, Polymerase gene fragments, Positive cells, Positive signal, Present study, Primary target, Protein expression, Protein gene, Receptor, Renal tubule, Respiratory diseases, Respiratory syndrome, Respiratory system, Sars, Sars cases, Sars coronavirus, Sars epidemics, Sars patients, Second hospital, Small intestine, Standard saline citrate, Sweat gland, Sweat gland cells, Syndrome, Testis, Thoracic aortic aneurysm, Tissue sections, Total number, Transmission electron microscopy, Tubule, Uninfected vero, Viral, Viral immunology, Viral inclusion body, Virus particles, Yanqing ding, York blood center, Zhongshan university.
Abstract
We previously identified the major pathological changes in the respiratory and immune systems of patients who died of severe acute respiratory syndrome (SARS) but gained little information on the organ distribution of SARS‐associated coronavirus (SARS‐CoV). In the present study, we used a murine monoclonal antibody specific for SARS‐CoV nucleoprotein, and probes specific for a SARS‐CoV RNA polymerase gene fragment, for immunohistochemistry and in situ hybridization, respectively, to detect SARS‐CoV systematically in tissues from patients who died of SARS. SARS‐CoV was found in lung, trachea/bronchus, stomach, small intestine, distal convoluted renal tubule, sweat gland, parathyroid, pituitary, pancreas, adrenal gland, liver and cerebrum, but was not detected in oesophagus, spleen, lymph node, bone marrow, heart, aorta, cerebellum, thyroid, testis, ovary, uterus or muscle. These results suggest that, in addition to the respiratory system, the gastrointestinal tract and other organs with detectable SARS‐CoV may also be targets of SARS‐CoV infection. The pathological changes in these organs may be caused directly by the cytopathic effect mediated by local replication of the SARS‐CoV; or indirectly as a result of systemic responses to respiratory failure or the harmful immune response induced by viral infection. In addition to viral spread through a respiratory route, SARS‐CoV in the intestinal tract, kidney and sweat glands may be excreted via faeces, urine and sweat, thereby leading to virus transmission. This study provides important information for understanding the pathogenesis of SARS‐CoV infection and sheds light on possible virus transmission pathways. This data will be useful for designing new strategies for prevention and treatment of SARS. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Url:
- https://api.istex.fr/ark:/67375/WNG-R04BCWC9-K/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167761
DOI: 10.1002/path.1560
Affiliations:
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Le document en format XML
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<author><name sortKey="Li, Xin" sort="Li, Xin" uniqKey="Li X" first="Xin" last="Li">Xin Li</name>
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<author><name sortKey="Kang, Wei" sort="Kang, Wei" uniqKey="Kang W" first="Wei" last="Kang">Wei Kang</name>
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<author><name sortKey="Weng, Desheng" sort="Weng, Desheng" uniqKey="Weng D" first="Desheng" last="Weng">Desheng Weng</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term>
<term>DNA-Directed RNA Polymerases (genetics)</term>
<term>Female</term>
<term>Humans</term>
<term>Immunohistochemistry (methods)</term>
<term>In Situ Hybridization (methods)</term>
<term>Lung (virology)</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Nucleoproteins (analysis)</term>
<term>RNA, Viral (genetics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Severe Acute Respiratory Syndrome (transmission)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Viral Proteins (analysis)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>DNA-directed RNA polymerases (génétique)</term>
<term>Femelle</term>
<term>Humains</term>
<term>Hybridation in situ ()</term>
<term>Immunohistochimie ()</term>
<term>Mâle</term>
<term>Nucléoprotéines (analyse)</term>
<term>Poumon (virologie)</term>
<term>Protéines virales (analyse)</term>
<term>RT-PCR ()</term>
<term>Syndrome respiratoire aigu sévère (transmission)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Virus du SRAS (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Nucleoproteins</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA-Directed RNA Polymerases</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Nucléoprotéines</term>
<term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>DNA-directed RNA polymerases</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunohistochemistry</term>
<term>In Situ Hybridization</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Poumon</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Ace2</term>
<term>Acidophilic cells</term>
<term>Adrenal gland</term>
<term>Adult</term>
<term>Assay</term>
<term>Autopsy tissues</term>
<term>Bone marrow</term>
<term>Central vein</term>
<term>Cerebral neurones</term>
<term>Control patients</term>
<term>Coronavirus</term>
<term>Cytopathic effect</term>
<term>Ding</term>
<term>Eighth hospital</term>
<term>Epithelial</term>
<term>Epithelial cells</term>
<term>Epithelium</term>
<term>Family members</term>
<term>Fang hospital</term>
<term>Female</term>
<term>Functional receptor</term>
<term>Gastric parietal cells</term>
<term>Gastrointestinal system</term>
<term>Gastrointestinal tract</term>
<term>Gene fragments</term>
<term>Great britain</term>
<term>Guangdong province</term>
<term>Guangzhou</term>
<term>Guangzhou city</term>
<term>Guangzhou institute</term>
<term>Health administration</term>
<term>Humans</term>
<term>Inclusion bodies</term>
<term>Infectious diseases</term>
<term>John wiley sons</term>
<term>Kimball research institute</term>
<term>Light microscope</term>
<term>Little information</term>
<term>Lung tissue</term>
<term>Lung tissues</term>
<term>Lymph node</term>
<term>Male</term>
<term>Medical university</term>
<term>Middle Aged</term>
<term>Natural sciences</term>
<term>Neutral formaldehyde</term>
<term>Novel coronavirus</term>
<term>Organ distribution</term>
<term>Other diseases</term>
<term>Other organs</term>
<term>Other people</term>
<term>Pancreatic acinar cells</term>
<term>Pathol</term>
<term>Pathological changes</term>
<term>Pathological society</term>
<term>Polymerase</term>
<term>Polymerase gene expression</term>
<term>Polymerase gene fragment</term>
<term>Polymerase gene fragments</term>
<term>Positive cells</term>
<term>Positive signal</term>
<term>Present study</term>
<term>Primary target</term>
<term>Protein expression</term>
<term>Protein gene</term>
<term>Receptor</term>
<term>Renal tubule</term>
<term>Respiratory diseases</term>
<term>Respiratory syndrome</term>
<term>Respiratory system</term>
<term>Sars</term>
<term>Sars cases</term>
<term>Sars coronavirus</term>
<term>Sars epidemics</term>
<term>Sars patients</term>
<term>Second hospital</term>
<term>Small intestine</term>
<term>Standard saline citrate</term>
<term>Sweat gland</term>
<term>Sweat gland cells</term>
<term>Syndrome</term>
<term>Testis</term>
<term>Thoracic aortic aneurysm</term>
<term>Tissue sections</term>
<term>Total number</term>
<term>Transmission electron microscopy</term>
<term>Tubule</term>
<term>Uninfected vero</term>
<term>Viral</term>
<term>Viral immunology</term>
<term>Viral inclusion body</term>
<term>Virus particles</term>
<term>Yanqing ding</term>
<term>York blood center</term>
<term>Zhongshan university</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Femelle</term>
<term>Humains</term>
<term>Hybridation in situ</term>
<term>Immunohistochimie</term>
<term>Mâle</term>
<term>RT-PCR</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We previously identified the major pathological changes in the respiratory and immune systems of patients who died of severe acute respiratory syndrome (SARS) but gained little information on the organ distribution of SARS‐associated coronavirus (SARS‐CoV). In the present study, we used a murine monoclonal antibody specific for SARS‐CoV nucleoprotein, and probes specific for a SARS‐CoV RNA polymerase gene fragment, for immunohistochemistry and in situ hybridization, respectively, to detect SARS‐CoV systematically in tissues from patients who died of SARS. SARS‐CoV was found in lung, trachea/bronchus, stomach, small intestine, distal convoluted renal tubule, sweat gland, parathyroid, pituitary, pancreas, adrenal gland, liver and cerebrum, but was not detected in oesophagus, spleen, lymph node, bone marrow, heart, aorta, cerebellum, thyroid, testis, ovary, uterus or muscle. These results suggest that, in addition to the respiratory system, the gastrointestinal tract and other organs with detectable SARS‐CoV may also be targets of SARS‐CoV infection. The pathological changes in these organs may be caused directly by the cytopathic effect mediated by local replication of the SARS‐CoV; or indirectly as a result of systemic responses to respiratory failure or the harmful immune response induced by viral infection. In addition to viral spread through a respiratory route, SARS‐CoV in the intestinal tract, kidney and sweat glands may be excreted via faeces, urine and sweat, thereby leading to virus transmission. This study provides important information for understanding the pathogenesis of SARS‐CoV infection and sheds light on possible virus transmission pathways. This data will be useful for designing new strategies for prevention and treatment of SARS. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>Guangdong</li>
<li>État de New York</li>
</region>
<settlement><li>Jiangmen</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Ding, Yanqing" sort="Ding, Yanqing" uniqKey="Ding Y" first="Yanqing" last="Ding">Yanqing Ding</name>
</region>
<name sortKey="Cai, Junjie" sort="Cai, Junjie" uniqKey="Cai J" first="Junjie" last="Cai">Junjie Cai</name>
<name sortKey="Che, Xiaoyan" sort="Che, Xiaoyan" uniqKey="Che X" first="Xiaoyan" last="Che">Xiaoyan Che</name>
<name sortKey="Geng, Jian" sort="Geng, Jian" uniqKey="Geng J" first="Jian" last="Geng">Jian Geng</name>
<name sortKey="Han, Huixia" sort="Han, Huixia" uniqKey="Han H" first="Huixia" last="Han">Huixia Han</name>
<name sortKey="He, Li" sort="He, Li" uniqKey="He L" first="Li" last="He">Li He</name>
<name sortKey="Hou, Jinlin" sort="Hou, Jinlin" uniqKey="Hou J" first="Jinlin" last="Hou">Jinlin Hou</name>
<name sortKey="Huang, Zhongxi" sort="Huang, Zhongxi" uniqKey="Huang Z" first="Zhongxi" last="Huang">Zhongxi Huang</name>
<name sortKey="Kang, Wei" sort="Kang, Wei" uniqKey="Kang W" first="Wei" last="Kang">Wei Kang</name>
<name sortKey="Li, Xin" sort="Li, Xin" uniqKey="Li X" first="Xin" last="Li">Xin Li</name>
<name sortKey="Li, Zhuguo" sort="Li, Zhuguo" uniqKey="Li Z" first="Zhuguo" last="Li">Zhuguo Li</name>
<name sortKey="Liang, Ping" sort="Liang, Ping" uniqKey="Liang P" first="Ping" last="Liang">Ping Liang</name>
<name sortKey="Qiu, Liwen" sort="Qiu, Liwen" uniqKey="Qiu L" first="Liwen" last="Qiu">Liwen Qiu</name>
<name sortKey="Shen, Hong" sort="Shen, Hong" uniqKey="Shen H" first="Hong" last="Shen">Hong Shen</name>
<name sortKey="Wang, Huijun" sort="Wang, Huijun" uniqKey="Wang H" first="Huijun" last="Wang">Huijun Wang</name>
<name sortKey="Weng, Desheng" sort="Weng, Desheng" uniqKey="Weng D" first="Desheng" last="Weng">Desheng Weng</name>
<name sortKey="Zhang, Qingling" sort="Zhang, Qingling" uniqKey="Zhang Q" first="Qingling" last="Zhang">Qingling Zhang</name>
</country>
<country name="États-Unis"><region name="État de New York"><name sortKey="Ding, Yanqing" sort="Ding, Yanqing" uniqKey="Ding Y" first="Yanqing" last="Ding">Yanqing Ding</name>
</region>
<name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
<name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
</country>
</tree>
</affiliations>
</record>
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