Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in SARS patients: implications for pathogenesis and virus transmission pathways.
Identifieur interne : 000809 ( Ncbi/Merge ); précédent : 000808; suivant : 000810Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in SARS patients: implications for pathogenesis and virus transmission pathways.
Auteurs : Yanqing Ding [République populaire de Chine] ; Li He ; Qingling Zhang ; Zhongxi Huang ; Xiaoyan Che ; Jinlin Hou ; Huijun Wang ; Hong Shen ; Liwen Qiu ; Zhuguo Li ; Jian Geng ; Junjie Cai ; Huixia Han ; Xin Li ; Wei Kang ; Desheng Weng ; Ping Liang ; Shibo JiangSource :
- The Journal of pathology [ 0022-3417 ] ; 2004.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Adulte, Adulte d'âge moyen, DNA-directed RNA polymerases (génétique), Femelle, Humains, Hybridation in situ (), Immunohistochimie (), Mâle, Nucléoprotéines (analyse), Poumon (virologie), Protéines virales (analyse), RT-PCR (), Syndrome respiratoire aigu sévère (transmission), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (isolement et purification).
- MESH :
- analyse : Nucléoprotéines, Protéines virales.
- génétique : ARN viral, DNA-directed RNA polymerases.
- isolement et purification : Virus du SRAS.
- virologie : Poumon, Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Femelle, Humains, Hybridation in situ, Immunohistochimie, Mâle, RT-PCR, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Adult, DNA-Directed RNA Polymerases (genetics), Female, Humans, Immunohistochemistry (methods), In Situ Hybridization (methods), Lung (virology), Male, Middle Aged, Nucleoproteins (analysis), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (transmission), Severe Acute Respiratory Syndrome (virology), Viral Proteins (analysis).
- MESH :
- chemical , analysis : Nucleoproteins, Viral Proteins.
- chemical , genetics : DNA-Directed RNA Polymerases, RNA, Viral.
- isolation & purification : SARS Virus.
- methods : Immunohistochemistry, In Situ Hybridization, Reverse Transcriptase Polymerase Chain Reaction.
- transmission : Severe Acute Respiratory Syndrome.
- virology : Lung, Severe Acute Respiratory Syndrome.
- Adult, Female, Humans, Male, Middle Aged.
Abstract
We previously identified the major pathological changes in the respiratory and immune systems of patients who died of severe acute respiratory syndrome (SARS) but gained little information on the organ distribution of SARS-associated coronavirus (SARS-CoV). In the present study, we used a murine monoclonal antibody specific for SARS-CoV nucleoprotein, and probes specific for a SARS-CoV RNA polymerase gene fragment, for immunohistochemistry and in situ hybridization, respectively, to detect SARS-CoV systematically in tissues from patients who died of SARS. SARS-CoV was found in lung, trachea/bronchus, stomach, small intestine, distal convoluted renal tubule, sweat gland, parathyroid, pituitary, pancreas, adrenal gland, liver and cerebrum, but was not detected in oesophagus, spleen, lymph node, bone marrow, heart, aorta, cerebellum, thyroid, testis, ovary, uterus or muscle. These results suggest that, in addition to the respiratory system, the gastrointestinal tract and other organs with detectable SARS-CoV may also be targets of SARS-CoV infection. The pathological changes in these organs may be caused directly by the cytopathic effect mediated by local replication of the SARS-CoV; or indirectly as a result of systemic responses to respiratory failure or the harmful immune response induced by viral infection. In addition to viral spread through a respiratory route, SARS-CoV in the intestinal tract, kidney and sweat glands may be excreted via faeces, urine and sweat, thereby leading to virus transmission. This study provides important information for understanding the pathogenesis of SARS-CoV infection and sheds light on possible virus transmission pathways. This data will be useful for designing new strategies for prevention and treatment of SARS.
DOI: 10.1002/path.1560
PubMed: 15141376
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pubmed:15141376Le document en format XML
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first="Yanqing" last="Ding">Yanqing Ding</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pathology, Nan Fang Hospital, First Military Medical University, Guangzhou, China. dyq@fimmu.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Nan Fang Hospital, First Military Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="He, Li" sort="He, Li" uniqKey="He L" first="Li" last="He">Li He</name></author><author><name sortKey="Zhang, Qingling" sort="Zhang, Qingling" uniqKey="Zhang Q" first="Qingling" last="Zhang">Qingling Zhang</name></author><author><name sortKey="Huang, Zhongxi" sort="Huang, Zhongxi" uniqKey="Huang Z" first="Zhongxi" last="Huang">Zhongxi Huang</name></author><author><name sortKey="Che, Xiaoyan" sort="Che, Xiaoyan" uniqKey="Che X" 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sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We previously identified the major pathological changes in the respiratory and immune systems of patients who died of severe acute respiratory syndrome (SARS) but gained little information on the organ distribution of SARS-associated coronavirus (SARS-CoV). In the present study, we used a murine monoclonal antibody specific for SARS-CoV nucleoprotein, and probes specific for a SARS-CoV RNA polymerase gene fragment, for immunohistochemistry and in situ hybridization, respectively, to detect SARS-CoV systematically in tissues from patients who died of SARS. SARS-CoV was found in lung, trachea/bronchus, stomach, small intestine, distal convoluted renal tubule, sweat gland, parathyroid, pituitary, pancreas, adrenal gland, liver and cerebrum, but was not detected in oesophagus, spleen, lymph node, bone marrow, heart, aorta, cerebellum, thyroid, testis, ovary, uterus or muscle. These results suggest that, in addition to the respiratory system, the gastrointestinal tract and other organs with detectable SARS-CoV may also be targets of SARS-CoV infection. The pathological changes in these organs may be caused directly by the cytopathic effect mediated by local replication of the SARS-CoV; or indirectly as a result of systemic responses to respiratory failure or the harmful immune response induced by viral infection. In addition to viral spread through a respiratory route, SARS-CoV in the intestinal tract, kidney and sweat glands may be excreted via faeces, urine and sweat, thereby leading to virus transmission. This study provides important information for understanding the pathogenesis of SARS-CoV infection and sheds light on possible virus transmission pathways. This data will be useful for designing new strategies for prevention and treatment of SARS.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15141376</PMID><DateCompleted><Year>2004</Year><Month>07</Month><Day>19</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-3417</ISSN><JournalIssue CitedMedium="Print"><Volume>203</Volume><Issue>2</Issue><PubDate><Year>2004</Year><Month>Jun</Month></PubDate></JournalIssue><Title>The Journal of pathology</Title><ISOAbbreviation>J. Pathol.</ISOAbbreviation></Journal><ArticleTitle>Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in SARS patients: implications for pathogenesis and virus transmission pathways.</ArticleTitle><Pagination><MedlinePgn>622-30</MedlinePgn></Pagination><Abstract><AbstractText>We previously identified the major pathological changes in the respiratory and immune systems of patients who died of severe acute respiratory syndrome (SARS) but gained little information on the organ distribution of SARS-associated coronavirus (SARS-CoV). In the present study, we used a murine monoclonal antibody specific for SARS-CoV nucleoprotein, and probes specific for a SARS-CoV RNA polymerase gene fragment, for immunohistochemistry and in situ hybridization, respectively, to detect SARS-CoV systematically in tissues from patients who died of SARS. SARS-CoV was found in lung, trachea/bronchus, stomach, small intestine, distal convoluted renal tubule, sweat gland, parathyroid, pituitary, pancreas, adrenal gland, liver and cerebrum, but was not detected in oesophagus, spleen, lymph node, bone marrow, heart, aorta, cerebellum, thyroid, testis, ovary, uterus or muscle. These results suggest that, in addition to the respiratory system, the gastrointestinal tract and other organs with detectable SARS-CoV may also be targets of SARS-CoV infection. The pathological changes in these organs may be caused directly by the cytopathic effect mediated by local replication of the SARS-CoV; or indirectly as a result of systemic responses to respiratory failure or the harmful immune response induced by viral infection. In addition to viral spread through a respiratory route, SARS-CoV in the intestinal tract, kidney and sweat glands may be excreted via faeces, urine and sweat, thereby leading to virus transmission. This study provides important information for understanding the pathogenesis of SARS-CoV infection and sheds light on possible virus transmission pathways. This data will be useful for designing new strategies for prevention and treatment of SARS.</AbstractText><CopyrightInformation>Copyright 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Yanqing</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Pathology, Nan Fang Hospital, First Military Medical University, Guangzhou, China. dyq@fimmu.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Li</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Qingling</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Zhongxi</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Che</LastName><ForeName>Xiaoyan</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Hou</LastName><ForeName>Jinlin</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Huijun</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Hong</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Qiu</LastName><ForeName>Liwen</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Zhuguo</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Geng</LastName><ForeName>Jian</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>Junjie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Huixia</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xin</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Weng</LastName><ForeName>Desheng</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Ping</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Shibo</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Pathol</MedlineTA><NlmUniqueID>0204634</NlmUniqueID><ISSNLinking>0022-3417</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009698">Nucleoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.6</RegistryNumber><NameOfSubstance UI="D012321">DNA-Directed RNA Polymerases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012321" MajorTopicYN="N">DNA-Directed RNA Polymerases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017403" MajorTopicYN="N">In Situ Hybridization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009698" MajorTopicYN="N">Nucleoproteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000635" MajorTopicYN="N">transmission</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>5</Month><Day>14</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>7</Month><Day>20</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>5</Month><Day>14</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15141376</ArticleId><ArticleId IdType="doi">10.1002/path.1560</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Cai, Junjie" sort="Cai, Junjie" uniqKey="Cai J" first="Junjie" last="Cai">Junjie Cai</name><name sortKey="Che, Xiaoyan" sort="Che, Xiaoyan" uniqKey="Che X" first="Xiaoyan" last="Che">Xiaoyan Che</name><name sortKey="Geng, Jian" sort="Geng, Jian" uniqKey="Geng J" first="Jian" last="Geng">Jian Geng</name><name sortKey="Han, Huixia" sort="Han, Huixia" uniqKey="Han H" first="Huixia" last="Han">Huixia Han</name><name sortKey="He, Li" sort="He, Li" uniqKey="He L" first="Li" last="He">Li He</name><name sortKey="Hou, Jinlin" sort="Hou, Jinlin" uniqKey="Hou J" first="Jinlin" last="Hou">Jinlin Hou</name><name sortKey="Huang, Zhongxi" sort="Huang, Zhongxi" uniqKey="Huang Z" first="Zhongxi" last="Huang">Zhongxi Huang</name><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Kang, Wei" sort="Kang, Wei" uniqKey="Kang W" first="Wei" last="Kang">Wei Kang</name><name sortKey="Li, Xin" sort="Li, Xin" uniqKey="Li X" first="Xin" last="Li">Xin Li</name><name sortKey="Li, Zhuguo" sort="Li, Zhuguo" uniqKey="Li Z" first="Zhuguo" last="Li">Zhuguo Li</name><name sortKey="Liang, Ping" sort="Liang, Ping" uniqKey="Liang P" first="Ping" last="Liang">Ping Liang</name><name sortKey="Qiu, Liwen" sort="Qiu, Liwen" uniqKey="Qiu L" first="Liwen" last="Qiu">Liwen Qiu</name><name sortKey="Shen, Hong" sort="Shen, Hong" uniqKey="Shen H" first="Hong" last="Shen">Hong Shen</name><name sortKey="Wang, Huijun" sort="Wang, Huijun" uniqKey="Wang H" first="Huijun" last="Wang">Huijun Wang</name><name sortKey="Weng, Desheng" sort="Weng, Desheng" uniqKey="Weng D" first="Desheng" last="Weng">Desheng Weng</name><name sortKey="Zhang, Qingling" sort="Zhang, Qingling" uniqKey="Zhang Q" first="Qingling" last="Zhang">Qingling Zhang</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Ding, Yanqing" sort="Ding, Yanqing" uniqKey="Ding Y" first="Yanqing" last="Ding">Yanqing Ding</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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