A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys
Identifieur interne : 002163 ( Main/Exploration ); précédent : 002162; suivant : 002164A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys
Auteurs : Andrea Balboni [Italie] ; Laura Gallina [Italie] ; Alessandra Palladini [Italie] ; Santino Prosperi [Italie] ; Mara Battilani [Italie]Source :
- The Scientific World Journal [ 2356-6140 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus, Virus du SRAS.
- pathogénicité : Coronavirus.
- virologie : Chiroptera, Infections à coronavirus.
- Animaux, Réaction de polymérisation en chaine en temps réel.
English descriptors
- KwdEn :
- MESH :
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus, SARS Virus.
- pathogenicity : Coronavirus.
- virology : Chiroptera, Coronavirus Infections.
- Animals, Real-Time Polymerase Chain Reaction.
Abstract
Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (
Url:
DOI: 10.1100/2012/989514
PubMed: 22654650
PubMed Central: 3353321
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 001475
- to stream Pmc, to step Curation: 001475
- to stream Pmc, to step Checkpoint: 000B67
- to stream PubMed, to step Corpus: 001353
- to stream PubMed, to step Curation: 001353
- to stream PubMed, to step Checkpoint: 001390
- to stream Ncbi, to step Merge: 002513
- to stream Ncbi, to step Curation: 002513
- to stream Ncbi, to step Checkpoint: 002513
- to stream Main, to step Merge: 002190
- to stream Main, to step Curation: 002163
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and
Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys</title><author><name sortKey="Balboni, Andrea" sort="Balboni, Andrea" uniqKey="Balboni A" first="Andrea" last="Balboni">Andrea Balboni</name><affiliation wicri:level="4"><nlm:aff id="I1">Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea><orgName type="university">Université de Bologne</orgName><placeName><settlement type="city">Bologne</settlement><region nuts="2" type="region">Émilie-Romagne</region></placeName></affiliation></author><author><name sortKey="Gallina, Laura" sort="Gallina, Laura" uniqKey="Gallina L" first="Laura" last="Gallina">Laura Gallina</name><affiliation wicri:level="4"><nlm:aff id="I1">Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea><orgName type="university">Université de Bologne</orgName><placeName><settlement type="city">Bologne</settlement><region nuts="2" type="region">Émilie-Romagne</region></placeName></affiliation></author><author><name sortKey="Palladini, Alessandra" sort="Palladini, Alessandra" uniqKey="Palladini A" first="Alessandra" last="Palladini">Alessandra Palladini</name><affiliation wicri:level="1"><nlm:aff id="I2">Dipartimento di Biologia Animale, Università degli Studi di Pavia, Via Taramelli 24, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia Animale, Università degli Studi di Pavia, Via Taramelli 24, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Prosperi, Santino" sort="Prosperi, Santino" uniqKey="Prosperi S" first="Santino" last="Prosperi">Santino Prosperi</name><affiliation wicri:level="4"><nlm:aff id="I1">Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea><orgName type="university">Université de Bologne</orgName><placeName><settlement type="city">Bologne</settlement><region nuts="2" type="region">Émilie-Romagne</region></placeName></affiliation></author><author><name sortKey="Battilani, Mara" sort="Battilani, Mara" uniqKey="Battilani M" first="Mara" last="Battilani">Mara Battilani</name><affiliation wicri:level="4"><nlm:aff id="I1">Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea><orgName type="university">Université de Bologne</orgName><placeName><settlement type="city">Bologne</settlement><region nuts="2" type="region">Émilie-Romagne</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22654650</idno><idno type="pmc">3353321</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353321</idno><idno type="RBID">PMC:3353321</idno><idno type="doi">10.1100/2012/989514</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">001475</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001475</idno><idno type="wicri:Area/Pmc/Curation">001475</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001475</idno><idno type="wicri:Area/Pmc/Checkpoint">000B67</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000B67</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:22654650</idno><idno type="wicri:Area/PubMed/Corpus">001353</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001353</idno><idno type="wicri:Area/PubMed/Curation">001353</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001353</idno><idno type="wicri:Area/PubMed/Checkpoint">001390</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001390</idno><idno type="wicri:Area/Ncbi/Merge">002513</idno><idno type="wicri:Area/Ncbi/Curation">002513</idno><idno type="wicri:Area/Ncbi/Checkpoint">002513</idno><idno type="wicri:doubleKey">2356-6140:2011:Balboni A:a:real:time</idno><idno type="wicri:Area/Main/Merge">002190</idno><idno type="wicri:Area/Main/Curation">002163</idno><idno type="wicri:Area/Main/Exploration">002163</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and
Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys</title><author><name sortKey="Balboni, Andrea" sort="Balboni, Andrea" uniqKey="Balboni A" first="Andrea" last="Balboni">Andrea Balboni</name><affiliation wicri:level="4"><nlm:aff id="I1">Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea><orgName type="university">Université de Bologne</orgName><placeName><settlement type="city">Bologne</settlement><region nuts="2" type="region">Émilie-Romagne</region></placeName></affiliation></author><author><name sortKey="Gallina, Laura" sort="Gallina, Laura" uniqKey="Gallina L" first="Laura" last="Gallina">Laura Gallina</name><affiliation wicri:level="4"><nlm:aff id="I1">Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea><orgName type="university">Université de Bologne</orgName><placeName><settlement type="city">Bologne</settlement><region nuts="2" type="region">Émilie-Romagne</region></placeName></affiliation></author><author><name sortKey="Palladini, Alessandra" sort="Palladini, Alessandra" uniqKey="Palladini A" first="Alessandra" last="Palladini">Alessandra Palladini</name><affiliation wicri:level="1"><nlm:aff id="I2">Dipartimento di Biologia Animale, Università degli Studi di Pavia, Via Taramelli 24, 27100 Pavia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Biologia Animale, Università degli Studi di Pavia, Via Taramelli 24, 27100 Pavia</wicri:regionArea><wicri:noRegion>27100 Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Prosperi, Santino" sort="Prosperi, Santino" uniqKey="Prosperi S" first="Santino" last="Prosperi">Santino Prosperi</name><affiliation wicri:level="4"><nlm:aff id="I1">Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea><orgName type="university">Université de Bologne</orgName><placeName><settlement type="city">Bologne</settlement><region nuts="2" type="region">Émilie-Romagne</region></placeName></affiliation></author><author><name sortKey="Battilani, Mara" sort="Battilani, Mara" uniqKey="Battilani M" first="Mara" last="Battilani">Mara Battilani</name><affiliation wicri:level="4"><nlm:aff id="I1">Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea><orgName type="university">Université de Bologne</orgName><placeName><settlement type="city">Bologne</settlement><region nuts="2" type="region">Émilie-Romagne</region></placeName></affiliation></author></analytic><series><title level="j">The Scientific World Journal</title><idno type="ISSN">2356-6140</idno><idno type="eISSN">1537-744X</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chiroptera (virology)</term><term>Coronavirus (genetics)</term><term>Coronavirus (pathogenicity)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Real-Time Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Chiroptera (virologie)</term><term>Coronavirus (génétique)</term><term>Coronavirus (pathogénicité)</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Réaction de polymérisation en chaine en temps réel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (<italic>Rhinolophus ferrumequinum</italic>) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Peiris, Jsm" uniqKey="Peiris J">JSM Peiris</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name></author><author><name sortKey="Oberste, Ms" uniqKey="Oberste M">MS Oberste</name></author><author><name sortKey="Monroe, Ss" uniqKey="Monroe S">SS Monroe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name></author><author><name sortKey="Hu, Z" uniqKey="Hu Z">Z Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Balboni, A" uniqKey="Balboni A">A Balboni</name></author><author><name sortKey="Palladini, A" uniqKey="Palladini A">A Palladini</name></author><author><name sortKey="Bogliani, G" uniqKey="Bogliani G">G Bogliani</name></author><author><name sortKey="Battilani, M" uniqKey="Battilani M">M Battilani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name></author><author><name sortKey="Gloza Rausch, F" uniqKey="Gloza Rausch F">F Gloza-Rausch</name></author><author><name sortKey="Glende, J" uniqKey="Glende J">J Glende</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name></author><author><name sortKey="Yu, M" uniqKey="Yu M">M Yu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Quan, Pl" uniqKey="Quan P">PL Quan</name></author><author><name sortKey="Firth, C" uniqKey="Firth C">C Firth</name></author><author><name sortKey="Street, C" uniqKey="Street C">C Street</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rihtari, D" uniqKey="Rihtari D">D Rihtarič</name></author><author><name sortKey="Hostnik, P" uniqKey="Hostnik P">P Hostnik</name></author><author><name sortKey="Steyer, A" uniqKey="Steyer A">A Steyer</name></author><author><name sortKey="Grom, J" uniqKey="Grom J">J Grom</name></author><author><name sortKey="Toplak, I" uniqKey="Toplak I">I Toplak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tong, S" uniqKey="Tong S">S Tong</name></author><author><name sortKey="Conrardy, C" uniqKey="Conrardy C">C Conrardy</name></author><author><name sortKey="Ruone, S" uniqKey="Ruone S">S Ruone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Skp" uniqKey="Lau S">SKP Lau</name></author><author><name sortKey="Woo, Pcy" uniqKey="Woo P">PCY Woo</name></author><author><name sortKey="Li, Ksm" uniqKey="Li K">KSM Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Skp" uniqKey="Lau S">SKP Lau</name></author><author><name sortKey="Woo, Pcy" uniqKey="Woo P">PCY Woo</name></author><author><name sortKey="Li, Ksm" uniqKey="Li K">KSM Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Skp" uniqKey="Lau S">SKP Lau</name></author><author><name sortKey="Li, Ksm" uniqKey="Li K">KSM Li</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Watanabe, S" uniqKey="Watanabe S">S Watanabe</name></author><author><name sortKey="Masangkay, Js" uniqKey="Masangkay J">JS Masangkay</name></author><author><name sortKey="Nagata, N" uniqKey="Nagata N">N Nagata</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pcy" uniqKey="Woo P">PCY Woo</name></author><author><name sortKey="Lau, Skp" uniqKey="Lau S">SKP Lau</name></author><author><name sortKey="Li, Ksm" uniqKey="Li K">KSM Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pcy" uniqKey="Woo P">PCY Woo</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Lau, Skp" uniqKey="Lau S">SKP Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, J" uniqKey="Yuan J">J Yuan</name></author><author><name sortKey="Hon, Cc" uniqKey="Hon C">CC Hon</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Escutenaire, S" uniqKey="Escutenaire S">S Escutenaire</name></author><author><name sortKey="Mohamed, N" uniqKey="Mohamed N">N Mohamed</name></author><author><name sortKey="Isaksson, M" uniqKey="Isaksson M">M Isaksson</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Battilani, M" uniqKey="Battilani M">M Battilani</name></author><author><name sortKey="Coradin, T" uniqKey="Coradin T">T Coradin</name></author><author><name sortKey="Scagliarini, A" uniqKey="Scagliarini A">A Scagliarini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Battilani, M" uniqKey="Battilani M">M Battilani</name></author><author><name sortKey="Balboni, A" uniqKey="Balboni A">A Balboni</name></author><author><name sortKey="Ustulin, M" uniqKey="Ustulin M">M Ustulin</name></author><author><name sortKey="Giunti, M" uniqKey="Giunti M">M Giunti</name></author><author><name sortKey="Scagliarini, A" uniqKey="Scagliarini A">A Scagliarini</name></author><author><name sortKey="Prosperi, S" uniqKey="Prosperi S">S Prosperi</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Italie</li></country><region><li>Émilie-Romagne</li></region><settlement><li>Bologne</li></settlement><orgName><li>Université de Bologne</li></orgName></list><tree><country name="Italie"><region name="Émilie-Romagne"><name sortKey="Balboni, Andrea" sort="Balboni, Andrea" uniqKey="Balboni A" first="Andrea" last="Balboni">Andrea Balboni</name></region><name sortKey="Battilani, Mara" sort="Battilani, Mara" uniqKey="Battilani M" first="Mara" last="Battilani">Mara Battilani</name><name sortKey="Gallina, Laura" sort="Gallina, Laura" uniqKey="Gallina L" first="Laura" last="Gallina">Laura Gallina</name><name sortKey="Palladini, Alessandra" sort="Palladini, Alessandra" uniqKey="Palladini A" first="Alessandra" last="Palladini">Alessandra Palladini</name><name sortKey="Prosperi, Santino" sort="Prosperi, Santino" uniqKey="Prosperi S" first="Santino" last="Prosperi">Santino Prosperi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002163 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002163 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3353321
|texte= A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and
Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22654650" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |