SrasV1, PubMed, Corpus, bibRecord, 001353

A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.

Identifieur interne : 001353 ( PubMed/Corpus ); précédent : 001352; suivant : 001354

A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.

Auteurs : Andrea Balboni ; Laura Gallina ; Alessandra Palladini ; Santino Prosperi ; Mara Battilani

Source :

TheScientificWorldJournal [ 1537-744X ] ; 2012.

RBID : pubmed:22654650

English descriptors

KwdEn :
- Animals, Chiroptera (virology), Coronavirus (genetics), Coronavirus (pathogenicity), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Real-Time Polymerase Chain Reaction, SARS Virus (genetics).
MESH :
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus, SARS Virus.
- pathogenicity : Coronavirus.
- virology : Chiroptera, Coronavirus Infections.
- Animals, Real-Time Polymerase Chain Reaction.

Abstract

Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (Rhinolophus ferrumequinum) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.

DOI: 10.1100/2012/989514
PubMed: 22654650

Links to Exploration step

pubmed:22654650

Le document en format XML

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<author><name sortKey="Balboni, Andrea" sort="Balboni, Andrea" uniqKey="Balboni A" first="Andrea" last="Balboni">Andrea Balboni</name>
<affiliation><nlm:affiliation>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy.</nlm:affiliation>
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<author><name sortKey="Gallina, Laura" sort="Gallina, Laura" uniqKey="Gallina L" first="Laura" last="Gallina">Laura Gallina</name>
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<author><name sortKey="Palladini, Alessandra" sort="Palladini, Alessandra" uniqKey="Palladini A" first="Alessandra" last="Palladini">Alessandra Palladini</name>
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<author><name sortKey="Prosperi, Santino" sort="Prosperi, Santino" uniqKey="Prosperi S" first="Santino" last="Prosperi">Santino Prosperi</name>
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<author><name sortKey="Battilani, Mara" sort="Battilani, Mara" uniqKey="Battilani M" first="Mara" last="Battilani">Mara Battilani</name>
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<author><name sortKey="Prosperi, Santino" sort="Prosperi, Santino" uniqKey="Prosperi S" first="Santino" last="Prosperi">Santino Prosperi</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
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<term>Coronavirus Infections (diagnosis)</term>
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<front><div type="abstract" xml:lang="en">Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (Rhinolophus ferrumequinum) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.</div>
</front>
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<ReferenceList><Reference><Citation>Nat Med. 2004 Dec;10(12 Suppl):S88-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15577937</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Immunol Med Microbiol. 2003 Nov 28;39(2):141-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14625097</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2005 Oct 28;310(5748):676-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16195424</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2006 Jul 20;351(1):180-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16647731</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Virol. 2007 Jan;152(1):41-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16941059</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2007 Feb;81(4):1574-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17121802</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2007 Oct 25;367(2):428-39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17617433</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virus Res. 2008 Apr;133(1):74-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17451830</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2009 Mar;15(3):482-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19239771</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Mar;84(6):2808-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20071579</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2010 Apr;91(Pt 4):1058-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20016037</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Virol. 2010 Apr;155(4):507-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20217155</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2010 Aug;16(8):1217-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20678314</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Nov;84(21):11336-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20686038</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2010;1(4). pii: e00208-10. doi: 10.1128/mBio.00208-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21063474</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Epidemiol Infect. 2011 Feb;139(2):216-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20478089</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Res. 2011;42:43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21366901</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14040-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16169905</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>

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A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.

A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki