A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.
Identifieur interne : 001390 ( PubMed/Checkpoint ); précédent : 001389; suivant : 001391A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.
Auteurs : Andrea Balboni [Italie] ; Laura Gallina ; Alessandra Palladini ; Santino Prosperi ; Mara BattilaniSource :
- TheScientificWorldJournal [ 1537-744X ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus, Virus du SRAS.
- pathogénicité : Coronavirus.
- virologie : Chiroptera, Infections à coronavirus.
- Animaux, Réaction de polymérisation en chaine en temps réel.
English descriptors
- KwdEn :
- MESH :
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus, SARS Virus.
- pathogenicity : Coronavirus.
- virology : Chiroptera, Coronavirus Infections.
- Animals, Real-Time Polymerase Chain Reaction.
Abstract
Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (Rhinolophus ferrumequinum) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.
DOI: 10.1100/2012/989514
PubMed: 22654650
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:22654650Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.</title>
<author><name sortKey="Balboni, Andrea" sort="Balboni, Andrea" uniqKey="Balboni A" first="Andrea" last="Balboni">Andrea Balboni</name>
<affiliation wicri:level="4"><nlm:affiliation>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea>
<orgName type="university">Université de Bologne</orgName>
<placeName><settlement type="city">Bologne</settlement>
<region nuts="2" type="region">Émilie-Romagne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gallina, Laura" sort="Gallina, Laura" uniqKey="Gallina L" first="Laura" last="Gallina">Laura Gallina</name>
</author>
<author><name sortKey="Palladini, Alessandra" sort="Palladini, Alessandra" uniqKey="Palladini A" first="Alessandra" last="Palladini">Alessandra Palladini</name>
</author>
<author><name sortKey="Prosperi, Santino" sort="Prosperi, Santino" uniqKey="Prosperi S" first="Santino" last="Prosperi">Santino Prosperi</name>
</author>
<author><name sortKey="Battilani, Mara" sort="Battilani, Mara" uniqKey="Battilani M" first="Mara" last="Battilani">Mara Battilani</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2012">2012</date>
<idno type="RBID">pubmed:22654650</idno>
<idno type="pmid">22654650</idno>
<idno type="doi">10.1100/2012/989514</idno>
<idno type="wicri:Area/PubMed/Corpus">001353</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001353</idno>
<idno type="wicri:Area/PubMed/Curation">001353</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001353</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001390</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001390</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.</title>
<author><name sortKey="Balboni, Andrea" sort="Balboni, Andrea" uniqKey="Balboni A" first="Andrea" last="Balboni">Andrea Balboni</name>
<affiliation wicri:level="4"><nlm:affiliation>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia</wicri:regionArea>
<orgName type="university">Université de Bologne</orgName>
<placeName><settlement type="city">Bologne</settlement>
<region nuts="2" type="region">Émilie-Romagne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gallina, Laura" sort="Gallina, Laura" uniqKey="Gallina L" first="Laura" last="Gallina">Laura Gallina</name>
</author>
<author><name sortKey="Palladini, Alessandra" sort="Palladini, Alessandra" uniqKey="Palladini A" first="Alessandra" last="Palladini">Alessandra Palladini</name>
</author>
<author><name sortKey="Prosperi, Santino" sort="Prosperi, Santino" uniqKey="Prosperi S" first="Santino" last="Prosperi">Santino Prosperi</name>
</author>
<author><name sortKey="Battilani, Mara" sort="Battilani, Mara" uniqKey="Battilani M" first="Mara" last="Battilani">Mara Battilani</name>
</author>
</analytic>
<series><title level="j">TheScientificWorldJournal</title>
<idno type="eISSN">1537-744X</idno>
<imprint><date when="2012" type="published">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Chiroptera (virology)</term>
<term>Coronavirus (genetics)</term>
<term>Coronavirus (pathogenicity)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (virology)</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>SARS Virus (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Chiroptera (virologie)</term>
<term>Coronavirus (génétique)</term>
<term>Coronavirus (pathogénicité)</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Virus du SRAS (génétique)</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term>
<term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term>
<term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Real-Time Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (Rhinolophus ferrumequinum) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22654650</PMID>
<DateCompleted><Year>2012</Year>
<Month>09</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1537-744X</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>2012</Volume>
<PubDate><Year>2012</Year>
</PubDate>
</JournalIssue>
<Title>TheScientificWorldJournal</Title>
<ISOAbbreviation>ScientificWorldJournal</ISOAbbreviation>
</Journal>
<ArticleTitle>A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.</ArticleTitle>
<Pagination><MedlinePgn>989514</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1100/2012/989514</ELocationID>
<Abstract><AbstractText>Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (Rhinolophus ferrumequinum) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Balboni</LastName>
<ForeName>Andrea</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gallina</LastName>
<ForeName>Laura</ForeName>
<Initials>L</Initials>
</Author>
<Author ValidYN="Y"><LastName>Palladini</LastName>
<ForeName>Alessandra</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Prosperi</LastName>
<ForeName>Santino</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Battilani</LastName>
<ForeName>Mara</ForeName>
<Initials>M</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2011</Year>
<Month>11</Month>
<Day>22</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>ScientificWorldJournal</MedlineTA>
<NlmUniqueID>101131163</NlmUniqueID>
<ISSNLinking>1537-744X</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002685" MajorTopicYN="N">Chiroptera</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year>
<Month>10</Month>
<Day>05</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2011</Year>
<Month>11</Month>
<Day>16</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2012</Year>
<Month>6</Month>
<Day>2</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2012</Year>
<Month>6</Month>
<Day>2</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2012</Year>
<Month>9</Month>
<Day>28</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">22654650</ArticleId>
<ArticleId IdType="doi">10.1100/2012/989514</ArticleId>
<ArticleId IdType="pmc">PMC3353321</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Nat Med. 2004 Dec;10(12 Suppl):S88-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15577937</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Immunol Med Microbiol. 2003 Nov 28;39(2):141-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14625097</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2005 Oct 28;310(5748):676-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16195424</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2006 Jul 20;351(1):180-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16647731</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Virol. 2007 Jan;152(1):41-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16941059</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2007 Feb;81(4):1574-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17121802</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2007 Oct 25;367(2):428-39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17617433</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virus Res. 2008 Apr;133(1):74-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17451830</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2009 Mar;15(3):482-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19239771</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Mar;84(6):2808-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20071579</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2010 Apr;91(Pt 4):1058-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20016037</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Virol. 2010 Apr;155(4):507-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20217155</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2010 Aug;16(8):1217-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20678314</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Nov;84(21):11336-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20686038</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2010;1(4). pii: e00208-10. doi: 10.1128/mBio.00208-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21063474</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Epidemiol Infect. 2011 Feb;139(2):216-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20478089</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Res. 2011;42:43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21366901</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14040-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16169905</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Italie</li>
</country>
<region><li>Émilie-Romagne</li>
</region>
<settlement><li>Bologne</li>
</settlement>
<orgName><li>Université de Bologne</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Battilani, Mara" sort="Battilani, Mara" uniqKey="Battilani M" first="Mara" last="Battilani">Mara Battilani</name>
<name sortKey="Gallina, Laura" sort="Gallina, Laura" uniqKey="Gallina L" first="Laura" last="Gallina">Laura Gallina</name>
<name sortKey="Palladini, Alessandra" sort="Palladini, Alessandra" uniqKey="Palladini A" first="Alessandra" last="Palladini">Alessandra Palladini</name>
<name sortKey="Prosperi, Santino" sort="Prosperi, Santino" uniqKey="Prosperi S" first="Santino" last="Prosperi">Santino Prosperi</name>
</noCountry>
<country name="Italie"><region name="Émilie-Romagne"><name sortKey="Balboni, Andrea" sort="Balboni, Andrea" uniqKey="Balboni A" first="Andrea" last="Balboni">Andrea Balboni</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001390 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 001390 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:22654650 |texte= A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:22654650" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |