A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys
Identifieur interne : 002513 ( Ncbi/Checkpoint ); précédent : 002512; suivant : 002514A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys
Auteurs : Andrea Balboni [Italie] ; Laura Gallina [Italie] ; Alessandra Palladini [Italie] ; Santino Prosperi [Italie] ; Mara Battilani [Italie]Source :
- The Scientific World Journal [ 2356-6140 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus, Virus du SRAS.
- pathogénicité : Coronavirus.
- virologie : Chiroptera, Infections à coronavirus.
- Animaux, Réaction de polymérisation en chaine en temps réel.
English descriptors
- KwdEn :
- MESH :
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus, SARS Virus.
- pathogenicity : Coronavirus.
- virology : Chiroptera, Coronavirus Infections.
- Animals, Real-Time Polymerase Chain Reaction.
Abstract
Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (
Url:
DOI: 10.1100/2012/989514
PubMed: 22654650
PubMed Central: 3353321
Affiliations:
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PMC:3353321Le document en format XML
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<series><title level="j">The Scientific World Journal</title>
<idno type="ISSN">2356-6140</idno>
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<term>Coronavirus (genetics)</term>
<term>Coronavirus (pathogenicity)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (virology)</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>SARS Virus (genetics)</term>
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<term>Chiroptera (virologie)</term>
<term>Coronavirus (génétique)</term>
<term>Coronavirus (pathogénicité)</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
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<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
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<front><div type="abstract" xml:lang="en"><p>Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (<italic>Rhinolophus ferrumequinum</italic>
) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.</p>
</div>
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