PandemieGrippaleV1, Main, Exploration, bibRecord, 001075

In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus

Identifieur interne : 001075 ( Main/Exploration ); précédent : 001074; suivant : 001076

In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus

Auteurs : L. Cegolon [Italie, Royaume-Uni] ; C. Salata [Italie] ; E. Piccoli [Italie] ; V. Juarez [France] ; G. Palu' [Italie] ; G. Mastrangelo [Italie] ; A. Calistri [Italie]

Source :

International journal of hygiene and environmental health [ 1438-4639 ] ; 2014.

RBID : Pascal:14-0075821

Descripteurs français

Pascal (Inist)
- Infection, Activité biologique, In vitro, Antiviral, 2009, Santé publique, Influenzavirus, Prévention, Traitement, Contrôle, Désinfection, Santé et environnement, Médecine environnementale, Pandémie, Grippe H1N1.
Wicri :
- topic : Santé publique.

English descriptors

KwdEn :
- 2009, Antiviral, Biological activity, Check, Disinfection, Environmental medicine, H1N1 influenza, Health and environment, In vitro, Infection, Influenzavirus, Prevention, Public health, Treatment.

Abstract

Influenza virus spreads via small particle aerosols, droplets and fomites, and since it can survive for a short time on surfaces, can be introduced into the nasal mucosa before it loses infectivity. The hypothiocyanite ion (OSCN^-), product of the lactoperoxidase/H₂O_2/SCN^- system of central airways, is emerging as an important molecule for innate defense mechanism against bacteria, fungi and viruses. Here we demonstrated that OSCN^- displays virucidal activity in vitro against the A/H1N1 2009 pandemic influenza virus. The concentration required to inhibit viral replication by 50% was 2 μM when virus were challenged directly with OSCN^- before cell inoculation. These values were even lower when inoculated cells were maintained in contact with enzyme free-OSCN^- in the culture medium. The last experimental conditions better reflect those of tracheobronchial mucosa, where HOSCN/OSCN^- is retained in the air-liquid interface and inactivates both the viruses approaching the epithelium from outside and those released from the inoculated cells after the replication cycle. Importantly no OSCN^- cytotoxicity was observed in the cellular system employed. The lack of toxicity in humans and the absence of damage on surfaces of fomites suggest a potential use of OSCN^- to avoid mucosal and environmental transmission of influenza virus. Since hypothiocyanite is normally present in human airways a low risk of viral resistance is envisaged. In vivo confirmatory studies are needed to evaluate the appropriate dose, regimen and formulation.

Affiliations:

France, Italie, Royaume-Uni
Angleterre, Auvergne-Rhône-Alpes, Grand Londres, Rhône-Alpes
Londres, Lyon

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000145
to stream PascalFrancis, to step Curation: 001C73
to stream PascalFrancis, to step Checkpoint: 000084
to stream Main, to step Merge: 001085
to stream Main, to step Curation: 001075

Le document en format XML

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<term>Environmental medicine</term>
<term>H1N1 influenza</term>
<term>Health and environment</term>
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<front><div type="abstract" xml:lang="en">Influenza virus spreads via small particle aerosols, droplets and fomites, and since it can survive for a short time on surfaces, can be introduced into the nasal mucosa before it loses infectivity. The hypothiocyanite ion (OSCN<sup>-</sup>
), product of the lactoperoxidase/H<sub>2</sub>
O<sub>2/</sub>
SCN<sup>-</sup>
 system of central airways, is emerging as an important molecule for innate defense mechanism against bacteria, fungi and viruses. Here we demonstrated that OSCN<sup>-</sup>
 displays virucidal activity in vitro against the A/H1N1 2009 pandemic influenza virus. The concentration required to inhibit viral replication by 50% was 2 μM when virus were challenged directly with OSCN<sup>-</sup>
 before cell inoculation. These values were even lower when inoculated cells were maintained in contact with enzyme free-OSCN<sup>-</sup>
 in the culture medium. The last experimental conditions better reflect those of tracheobronchial mucosa, where HOSCN/OSCN<sup>-</sup>
 is retained in the air-liquid interface and inactivates both the viruses approaching the epithelium from outside and those released from the inoculated cells after the replication cycle. Importantly no OSCN<sup>-</sup>
 cytotoxicity was observed in the cellular system employed. The lack of toxicity in humans and the absence of damage on surfaces of fomites suggest a potential use of OSCN<sup>-</sup>
 to avoid mucosal and environmental transmission of influenza virus. Since hypothiocyanite is normally present in human airways a low risk of viral resistance is envisaged. In vivo confirmatory studies are needed to evaluate the appropriate dose, regimen and formulation.</div>
</front>
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In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus

In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri