PandemieGrippaleV1, PascalFrancis, Curation, bibRecord, 001C73

In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus

Identifieur interne : 001C73 ( PascalFrancis/Curation ); précédent : 001C72; suivant : 001C74

In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus

Auteurs : L. Cegolon [Italie, Royaume-Uni] ; C. Salata [Italie] ; E. Piccoli [Italie] ; V. Juarez [France] ; G. Palu' [Italie] ; G. Mastrangelo [Italie] ; A. Calistri [Italie]

Source :

International journal of hygiene and environmental health [ 1438-4639 ] ; 2014.

RBID : Pascal:14-0075821

Descripteurs français

Pascal (Inist)
- Infection, Activité biologique, In vitro, Antiviral, 2009, Santé publique, Influenzavirus, Prévention, Traitement, Contrôle, Désinfection, Santé et environnement, Médecine environnementale, Pandémie, Grippe H1N1.
Wicri :
- topic : Santé publique.

English descriptors

KwdEn :
- 2009, Antiviral, Biological activity, Check, Disinfection, Environmental medicine, H1N1 influenza, Health and environment, In vitro, Infection, Influenzavirus, Prevention, Public health, Treatment.

Abstract

Influenza virus spreads via small particle aerosols, droplets and fomites, and since it can survive for a short time on surfaces, can be introduced into the nasal mucosa before it loses infectivity. The hypothiocyanite ion (OSCN^-), product of the lactoperoxidase/H₂O_2/SCN^- system of central airways, is emerging as an important molecule for innate defense mechanism against bacteria, fungi and viruses. Here we demonstrated that OSCN^- displays virucidal activity in vitro against the A/H1N1 2009 pandemic influenza virus. The concentration required to inhibit viral replication by 50% was 2 μM when virus were challenged directly with OSCN^- before cell inoculation. These values were even lower when inoculated cells were maintained in contact with enzyme free-OSCN^- in the culture medium. The last experimental conditions better reflect those of tracheobronchial mucosa, where HOSCN/OSCN^- is retained in the air-liquid interface and inactivates both the viruses approaching the epithelium from outside and those released from the inoculated cells after the replication cycle. Importantly no OSCN^- cytotoxicity was observed in the cellular system employed. The lack of toxicity in humans and the absence of damage on surfaces of fomites suggest a potential use of OSCN^- to avoid mucosal and environmental transmission of influenza virus. Since hypothiocyanite is normally present in human airways a low risk of viral resistance is envisaged. In vivo confirmatory studies are needed to evaluate the appropriate dose, regimen and formulation.

A01	`01`	`1`		`@0 1438-4639`
A03		`1`		`@0 Int. j. hyg. environ. health : (print)`
A05				`@2 217`
A06				`@2 1`
A08	`01`	`1`	`ENG`	`@1 In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus`
A11	`01`	`1`		`@1 CEGOLON (L.)`
A11	`02`	`1`		`@1 SALATA (C.)`
A11	`03`	`1`		`@1 PICCOLI (E.)`
A11	`04`	`1`		`@1 JUAREZ (V.)`
A11	`05`	`1`		`@1 PALU' (G.)`
A11	`06`	`1`		`@1 MASTRANGELO (G.)`
A11	`07`	`1`		`@1 CALISTRI (A.)`
A14	`01`			`@1 Padua University, Department of Molecular Medicine @2 Padua @3 ITA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut.`
A14	`02`			`@1 Imperial College London, School of Public Health, St. Mary's Campus @2 London @3 GBR @Z 1 aut.`
A14	`03`			`@1 Alaxia SAS @2 Lyon @3 FRA @Z 4 aut.`
A20				`@1 17-22`
A21				`@1 2014`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 7500 @5 354000505792760030`
A44				`@0 0000 @1 © 2014 INIST-CNRS. All rights reserved.`
A45				`@0 3/4 p.`
A47	`01`	`1`		`@0 14-0075821`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 International journal of hygiene and environmental health`
A66	`01`			`@0 DEU`
C01	`01`		`ENG`	@0 Influenza virus spreads via small particle aerosols, droplets and fomites, and since it can survive for a short time on surfaces, can be introduced into the nasal mucosa before it loses infectivity. The hypothiocyanite ion (OSCN^-), product of the lactoperoxidase/H₂O_2/SCN^- system of central airways, is emerging as an important molecule for innate defense mechanism against bacteria, fungi and viruses. Here we demonstrated that OSCN^- displays virucidal activity in vitro against the A/H1N1 2009 pandemic influenza virus. The concentration required to inhibit viral replication by 50% was 2 μM when virus were challenged directly with OSCN^- before cell inoculation. These values were even lower when inoculated cells were maintained in contact with enzyme free-OSCN^- in the culture medium. The last experimental conditions better reflect those of tracheobronchial mucosa, where HOSCN/OSCN^- is retained in the air-liquid interface and inactivates both the viruses approaching the epithelium from outside and those released from the inoculated cells after the replication cycle. Importantly no OSCN^- cytotoxicity was observed in the cellular system employed. The lack of toxicity in humans and the absence of damage on surfaces of fomites suggest a potential use of OSCN^- to avoid mucosal and environmental transmission of influenza virus. Since hypothiocyanite is normally present in human airways a low risk of viral resistance is envisaged. In vivo confirmatory studies are needed to evaluate the appropriate dose, regimen and formulation.
C02	`01`	`X`		`@0 002B03`
C02	`02`	`X`		`@0 002B30A02A`
C02	`03`	`X`		`@0 002B02S05`
C03	`01`	`X`	`FRE`	`@0 Infection @5 01`
C03	`01`	`X`	`ENG`	`@0 Infection @5 01`
C03	`01`	`X`	`SPA`	`@0 Infección @5 01`
C03	`02`	`X`	`FRE`	`@0 Activité biologique @5 02`
C03	`02`	`X`	`ENG`	`@0 Biological activity @5 02`
C03	`02`	`X`	`SPA`	`@0 Actividad biológica @5 02`
C03	`03`	`X`	`FRE`	`@0 In vitro @5 03`
C03	`03`	`X`	`ENG`	`@0 In vitro @5 03`
C03	`03`	`X`	`SPA`	`@0 In vitro @5 03`
C03	`04`	`X`	`FRE`	`@0 Antiviral @5 05`
C03	`04`	`X`	`ENG`	`@0 Antiviral @5 05`
C03	`04`	`X`	`SPA`	`@0 Antiviral @5 05`
C03	`05`	`X`	`FRE`	`@0 2009 @5 06`
C03	`05`	`X`	`ENG`	`@0 2009 @5 06`
C03	`05`	`X`	`SPA`	`@0 2009 @5 06`
C03	`06`	`X`	`FRE`	`@0 Santé publique @5 08`
C03	`06`	`X`	`ENG`	`@0 Public health @5 08`
C03	`06`	`X`	`SPA`	`@0 Salud pública @5 08`
C03	`07`	`X`	`FRE`	`@0 Influenzavirus @2 NW @5 09`
C03	`07`	`X`	`ENG`	`@0 Influenzavirus @2 NW @5 09`
C03	`07`	`X`	`SPA`	`@0 Influenzavirus @2 NW @5 09`
C03	`08`	`X`	`FRE`	`@0 Prévention @5 11`
C03	`08`	`X`	`ENG`	`@0 Prevention @5 11`
C03	`08`	`X`	`SPA`	`@0 Prevención @5 11`
C03	`09`	`X`	`FRE`	`@0 Traitement @5 12`
C03	`09`	`X`	`ENG`	`@0 Treatment @5 12`
C03	`09`	`X`	`SPA`	`@0 Tratamiento @5 12`
C03	`10`	`X`	`FRE`	`@0 Contrôle @5 17`
C03	`10`	`X`	`ENG`	`@0 Check @5 17`
C03	`10`	`X`	`SPA`	`@0 Control @5 17`
C03	`11`	`X`	`FRE`	`@0 Désinfection @5 18`
C03	`11`	`X`	`ENG`	`@0 Disinfection @5 18`
C03	`11`	`X`	`SPA`	`@0 Desinfección @5 18`
C03	`12`	`X`	`FRE`	`@0 Santé et environnement @5 25`
C03	`12`	`X`	`ENG`	`@0 Health and environment @5 25`
C03	`12`	`X`	`SPA`	`@0 Salud y medio ambiente @5 25`
C03	`13`	`X`	`FRE`	`@0 Médecine environnementale @5 26`
C03	`13`	`X`	`ENG`	`@0 Environmental medicine @5 26`
C03	`13`	`X`	`SPA`	`@0 Medicina ambiental @5 26`
C03	`14`	`X`	`FRE`	`@0 Pandémie @4 INC @5 86`
C03	`15`	`X`	`FRE`	`@0 Grippe H1N1 @4 CD @5 96`
C03	`15`	`X`	`ENG`	`@0 H1N1 influenza @4 CD @5 96`
C03	`15`	`X`	`SPA`	`@0 Gripe H1N1 @4 CD @5 96`
C07	`01`	`X`	`FRE`	`@0 Orthomyxoviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Orthomyxoviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Orthomyxoviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`02`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`02`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`03`	`X`	`FRE`	`@0 Pathologie de l'appareil respiratoire @5 37`
C07	`03`	`X`	`ENG`	`@0 Respiratory disease @5 37`
C07	`03`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 37`
C07	`04`	`X`	`FRE`	`@0 Virose @5 38`
C07	`04`	`X`	`ENG`	`@0 Viral disease @5 38`
C07	`04`	`X`	`SPA`	`@0 Virosis @5 38`
N21				`@1 104`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000145

Links to Exploration step

Pascal:14-0075821

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus</title>
<author><name sortKey="Cegolon, L" sort="Cegolon, L" uniqKey="Cegolon L" first="L." last="Cegolon">L. Cegolon</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Imperial College London, School of Public Health, St. Mary's Campus</s1>
<s2>London</s2>
<s3>GBR</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
</affiliation>
</author>
<author><name sortKey="Salata, C" sort="Salata, C" uniqKey="Salata C" first="C." last="Salata">C. Salata</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
<author><name sortKey="Piccoli, E" sort="Piccoli, E" uniqKey="Piccoli E" first="E." last="Piccoli">E. Piccoli</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
<author><name sortKey="Juarez, V" sort="Juarez, V" uniqKey="Juarez V" first="V." last="Juarez">V. Juarez</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Alaxia SAS</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Palu, G" sort="Palu, G" uniqKey="Palu G" first="G." last="Palu'">G. Palu'</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
<author><name sortKey="Mastrangelo, G" sort="Mastrangelo, G" uniqKey="Mastrangelo G" first="G." last="Mastrangelo">G. Mastrangelo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
<author><name sortKey="Calistri, A" sort="Calistri, A" uniqKey="Calistri A" first="A." last="Calistri">A. Calistri</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">14-0075821</idno>
<date when="2014">2014</date>
<idno type="stanalyst">PASCAL 14-0075821 INIST</idno>
<idno type="RBID">Pascal:14-0075821</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000145</idno>
<idno type="wicri:Area/PascalFrancis/Curation">001C73</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus</title>
<author><name sortKey="Cegolon, L" sort="Cegolon, L" uniqKey="Cegolon L" first="L." last="Cegolon">L. Cegolon</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Imperial College London, School of Public Health, St. Mary's Campus</s1>
<s2>London</s2>
<s3>GBR</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
</affiliation>
</author>
<author><name sortKey="Salata, C" sort="Salata, C" uniqKey="Salata C" first="C." last="Salata">C. Salata</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
<author><name sortKey="Piccoli, E" sort="Piccoli, E" uniqKey="Piccoli E" first="E." last="Piccoli">E. Piccoli</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
<author><name sortKey="Juarez, V" sort="Juarez, V" uniqKey="Juarez V" first="V." last="Juarez">V. Juarez</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Alaxia SAS</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Palu, G" sort="Palu, G" uniqKey="Palu G" first="G." last="Palu'">G. Palu'</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
<author><name sortKey="Mastrangelo, G" sort="Mastrangelo, G" uniqKey="Mastrangelo G" first="G." last="Mastrangelo">G. Mastrangelo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
<author><name sortKey="Calistri, A" sort="Calistri, A" uniqKey="Calistri A" first="A." last="Calistri">A. Calistri</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Italie</country>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">International journal of hygiene and environmental health</title>
<title level="j" type="abbreviated">Int. j. hyg. environ. health : (print)</title>
<idno type="ISSN">1438-4639</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">International journal of hygiene and environmental health</title>
<title level="j" type="abbreviated">Int. j. hyg. environ. health : (print)</title>
<idno type="ISSN">1438-4639</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>2009</term>
<term>Antiviral</term>
<term>Biological activity</term>
<term>Check</term>
<term>Disinfection</term>
<term>Environmental medicine</term>
<term>H1N1 influenza</term>
<term>Health and environment</term>
<term>In vitro</term>
<term>Infection</term>
<term>Influenzavirus</term>
<term>Prevention</term>
<term>Public health</term>
<term>Treatment</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Infection</term>
<term>Activité biologique</term>
<term>In vitro</term>
<term>Antiviral</term>
<term>2009</term>
<term>Santé publique</term>
<term>Influenzavirus</term>
<term>Prévention</term>
<term>Traitement</term>
<term>Contrôle</term>
<term>Désinfection</term>
<term>Santé et environnement</term>
<term>Médecine environnementale</term>
<term>Pandémie</term>
<term>Grippe H1N1</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Santé publique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Influenza virus spreads via small particle aerosols, droplets and fomites, and since it can survive for a short time on surfaces, can be introduced into the nasal mucosa before it loses infectivity. The hypothiocyanite ion (OSCN<sup>-</sup>
), product of the lactoperoxidase/H<sub>2</sub>
O<sub>2/</sub>
SCN<sup>-</sup>
 system of central airways, is emerging as an important molecule for innate defense mechanism against bacteria, fungi and viruses. Here we demonstrated that OSCN<sup>-</sup>
 displays virucidal activity in vitro against the A/H1N1 2009 pandemic influenza virus. The concentration required to inhibit viral replication by 50% was 2 μM when virus were challenged directly with OSCN<sup>-</sup>
 before cell inoculation. These values were even lower when inoculated cells were maintained in contact with enzyme free-OSCN<sup>-</sup>
 in the culture medium. The last experimental conditions better reflect those of tracheobronchial mucosa, where HOSCN/OSCN<sup>-</sup>
 is retained in the air-liquid interface and inactivates both the viruses approaching the epithelium from outside and those released from the inoculated cells after the replication cycle. Importantly no OSCN<sup>-</sup>
 cytotoxicity was observed in the cellular system employed. The lack of toxicity in humans and the absence of damage on surfaces of fomites suggest a potential use of OSCN<sup>-</sup>
 to avoid mucosal and environmental transmission of influenza virus. Since hypothiocyanite is normally present in human airways a low risk of viral resistance is envisaged. In vivo confirmatory studies are needed to evaluate the appropriate dose, regimen and formulation.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>1438-4639</s0>
</fA01>
<fA03 i2="1"><s0>Int. j. hyg. environ. health : (print)</s0>
</fA03>
<fA05><s2>217</s2>
</fA05>
<fA06><s2>1</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>CEGOLON (L.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>SALATA (C.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>PICCOLI (E.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>JUAREZ (V.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>PALU' (G.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>MASTRANGELO (G.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>CALISTRI (A.)</s1>
</fA11>
<fA14 i1="01"><s1>Padua University, Department of Molecular Medicine</s1>
<s2>Padua</s2>
<s3>ITA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Imperial College London, School of Public Health, St. Mary's Campus</s1>
<s2>London</s2>
<s3>GBR</s3>
<sZ>1 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Alaxia SAS</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
</fA14>
<fA20><s1>17-22</s1>
</fA20>
<fA21><s1>2014</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>7500</s2>
<s5>354000505792760030</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2014 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>3/4 p.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>14-0075821</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>International journal of hygiene and environmental health</s0>
</fA64>
<fA66 i1="01"><s0>DEU</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Influenza virus spreads via small particle aerosols, droplets and fomites, and since it can survive for a short time on surfaces, can be introduced into the nasal mucosa before it loses infectivity. The hypothiocyanite ion (OSCN<sup>-</sup>
), product of the lactoperoxidase/H<sub>2</sub>
O<sub>2/</sub>
SCN<sup>-</sup>
 system of central airways, is emerging as an important molecule for innate defense mechanism against bacteria, fungi and viruses. Here we demonstrated that OSCN<sup>-</sup>
 displays virucidal activity in vitro against the A/H1N1 2009 pandemic influenza virus. The concentration required to inhibit viral replication by 50% was 2 μM when virus were challenged directly with OSCN<sup>-</sup>
 before cell inoculation. These values were even lower when inoculated cells were maintained in contact with enzyme free-OSCN<sup>-</sup>
 in the culture medium. The last experimental conditions better reflect those of tracheobronchial mucosa, where HOSCN/OSCN<sup>-</sup>
 is retained in the air-liquid interface and inactivates both the viruses approaching the epithelium from outside and those released from the inoculated cells after the replication cycle. Importantly no OSCN<sup>-</sup>
 cytotoxicity was observed in the cellular system employed. The lack of toxicity in humans and the absence of damage on surfaces of fomites suggest a potential use of OSCN<sup>-</sup>
 to avoid mucosal and environmental transmission of influenza virus. Since hypothiocyanite is normally present in human airways a low risk of viral resistance is envisaged. In vivo confirmatory studies are needed to evaluate the appropriate dose, regimen and formulation.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B03</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002B30A02A</s0>
</fC02>
<fC02 i1="03" i2="X"><s0>002B02S05</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Infection</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Infection</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Infección</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Activité biologique</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Biological activity</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Actividad biológica</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>In vitro</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>In vitro</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>In vitro</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Antiviral</s0>
<s5>05</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Antiviral</s0>
<s5>05</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Antiviral</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>2009</s0>
<s5>06</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>2009</s0>
<s5>06</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>2009</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Santé publique</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Public health</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Salud pública</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Influenzavirus</s0>
<s2>NW</s2>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Influenzavirus</s0>
<s2>NW</s2>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Influenzavirus</s0>
<s2>NW</s2>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Prévention</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Prevention</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Prevención</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Traitement</s0>
<s5>12</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Treatment</s0>
<s5>12</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Tratamiento</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Contrôle</s0>
<s5>17</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Check</s0>
<s5>17</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Control</s0>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Désinfection</s0>
<s5>18</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Disinfection</s0>
<s5>18</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Desinfección</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Santé et environnement</s0>
<s5>25</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Health and environment</s0>
<s5>25</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Salud y medio ambiente</s0>
<s5>25</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Médecine environnementale</s0>
<s5>26</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Environmental medicine</s0>
<s5>26</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Medicina ambiental</s0>
<s5>26</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Pandémie</s0>
<s4>INC</s4>
<s5>86</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Grippe H1N1</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG"><s0>H1N1 influenza</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="15" i2="X" l="SPA"><s0>Gripe H1N1</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Orthomyxoviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Orthomyxoviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Orthomyxoviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Pathologie de l'appareil respiratoire</s0>
<s5>37</s5>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>37</s5>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>37</s5>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virose</s0>
<s5>38</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Viral disease</s0>
<s5>38</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virosis</s0>
<s5>38</s5>
</fC07>
<fN21><s1>104</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/PascalFrancis/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C73 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Curation/biblio.hfd -nk 001C73 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    PandemieGrippaleV1
   |flux=    PascalFrancis
   |étape=   Curation
   |type=    RBID
   |clé=     Pascal:14-0075821
   |texte=   In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus
}}

This area was generated with Dilib version V0.6.34.
Data generation: Wed Jun 10 11:04:28 2020. Site generation: Sun Mar 28 09:10:28 2021

	Serveur d'exploration sur les pandémies grippales
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les pandémies grippales

In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus

In vitro antiviral activity of hypothiocyanite against A/H1N1/2009 pandemic influenza virus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri