Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein
Identifieur interne : 001D38 ( Main/Merge ); précédent : 001D37; suivant : 001D39Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein
Auteurs : Hsin-Hou Chang [Taïwan] ; Po-Kong Chen [Taïwan] ; Guan-Ling Lin [Taïwan] ; Chun-Jen Wang [Taïwan] ; Chih-Hsien Liao [Taïwan] ; Yu-Cheng Hsiao [Taïwan] ; Jing-Hua Dong [Taïwan] ; Der-Shan Sun [Taïwan]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Antiviraux, Peptides.
- métabolisme : Glycoprotéine de spicule des coronavirus.
- physiologie : Virus du SRAS.
- Adhérence cellulaire, Animaux, Attachement viral, Humains, Liaison aux protéines, Lignée cellulaire, Sites de fixation.
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : Antiviral Agents, Peptides.
- chemical , metabolism : Spike Glycoprotein, Coronavirus.
- physiology : SARS Virus.
- Animals, Binding Sites, Cell Adhesion, Cell Line, Humans, Protein Binding, Virus Attachment.
Abstract
Cell adhesion assay is a simple method to identify the receptor-binding motif of the SARS-CoV spike. Synthetic peptides can be used to narrow the receptor-binding motif. The 436–445 amino acids are the minimum receptor-binding motif of the SARS-CoV spike. Cell adhesion assay can be developed as a high throughput system by using a 96-well MTT assay.
Url:
DOI: 10.1016/j.jviromet.2014.01.022
PubMed: 24530430
PubMed Central: 7113645
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PMC:7113645Le document en format XML
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<series><title level="j">Journal of Virological Methods</title>
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<term>Antiviral Agents (isolation & purification)</term>
<term>Binding Sites</term>
<term>Cell Adhesion</term>
<term>Cell Line</term>
<term>Humans</term>
<term>Peptides (isolation & purification)</term>
<term>Protein Binding</term>
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<term>Attachement viral</term>
<term>Glycoprotéine de spicule des coronavirus (métabolisme)</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Peptides (isolement et purification)</term>
<term>Sites de fixation</term>
<term>Virus du SRAS (physiologie)</term>
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<front><div type="abstract" xml:lang="en"><title>Highlights</title>
<p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label>
<p id="par0005">Cell adhesion assay is a simple method to identify the receptor-binding motif of the SARS-CoV spike.</p>
</list-item>
<list-item id="lsti0010"><label>•</label>
<p id="par0010">Synthetic peptides can be used to narrow the receptor-binding motif.</p>
</list-item>
<list-item id="lsti0015"><label>•</label>
<p id="par0015">The 436–445 amino acids are the minimum receptor-binding motif of the SARS-CoV spike.</p>
</list-item>
<list-item id="lsti0020"><label>•</label>
<p id="par0020">Cell adhesion assay can be developed as a high throughput system by using a 96-well MTT assay.</p>
</list-item>
</list>
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