Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein
Identifieur interne : 000E97 ( Pmc/Curation ); précédent : 000E96; suivant : 000E98Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein
Auteurs : Hsin-Hou Chang [Taïwan] ; Po-Kong Chen [Taïwan] ; Guan-Ling Lin [Taïwan] ; Chun-Jen Wang [Taïwan] ; Chih-Hsien Liao [Taïwan] ; Yu-Cheng Hsiao [Taïwan] ; Jing-Hua Dong [Taïwan] ; Der-Shan Sun [Taïwan]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2014.
Abstract
Cell adhesion assay is a simple method to identify the receptor-binding motif of the SARS-CoV spike. Synthetic peptides can be used to narrow the receptor-binding motif. The 436–445 amino acids are the minimum receptor-binding motif of the SARS-CoV spike. Cell adhesion assay can be developed as a high throughput system by using a 96-well MTT assay.
Url:
DOI: 10.1016/j.jviromet.2014.01.022
PubMed: 24530430
PubMed Central: 7113645
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level="a" type="main">Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein</title><author><name sortKey="Chang, Hsin Hou" sort="Chang, Hsin Hou" uniqKey="Chang H" first="Hsin-Hou" last="Chang">Hsin-Hou Chang</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Medical Sciences, Tzu-Chi University, Hualien</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Po Kong" sort="Chen, Po Kong" uniqKey="Chen P" first="Po-Kong" last="Chen">Po-Kong Chen</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Medical Sciences, Tzu-Chi University, Hualien</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Guan Ling" sort="Lin, Guan Ling" uniqKey="Lin G" first="Guan-Ling" last="Lin">Guan-Ling Lin</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Medical Sciences, Tzu-Chi University, Hualien</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Chun Jen" sort="Wang, Chun Jen" uniqKey="Wang C" first="Chun-Jen" last="Wang">Chun-Jen Wang</name><affiliation wicri:level="1"><nlm:aff id="aff0015">The Department of Life Sciences, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>The Department of Life Sciences, Tzu-Chi University, Hualien</wicri:regionArea></affiliation></author><author><name sortKey="Liao, Chih Hsien" sort="Liao, Chih Hsien" uniqKey="Liao C" first="Chih-Hsien" last="Liao">Chih-Hsien Liao</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea></affiliation></author><author><name sortKey="Hsiao, Yu Cheng" sort="Hsiao, Yu Cheng" uniqKey="Hsiao Y" first="Yu-Cheng" last="Hsiao">Yu-Cheng Hsiao</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea></affiliation></author><author><name sortKey="Dong, Jing Hua" sort="Dong, Jing Hua" uniqKey="Dong J" first="Jing-Hua" last="Dong">Jing-Hua Dong</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea></affiliation></author><author><name sortKey="Sun, Der Shan" sort="Sun, Der Shan" uniqKey="Sun D" first="Der-Shan" last="Sun">Der-Shan Sun</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Medical Sciences, Tzu-Chi University, Hualien</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of Virological Methods</title><idno type="ISSN">0166-0934</idno><idno type="eISSN">1879-0984</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Cell adhesion assay is a simple method to identify the receptor-binding motif of the SARS-CoV spike.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Synthetic peptides can be used to narrow the receptor-binding motif.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">The 436–445 amino acids are the minimum receptor-binding motif of the SARS-CoV spike.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">Cell adhesion assay can be developed as a high throughput system by using a 96-well MTT assay.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Babcock, G J" uniqKey="Babcock G">G.J. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol Methods</journal-id><journal-id journal-id-type="iso-abbrev">J. Virol. Methods</journal-id><journal-title-group><journal-title>Journal of Virological Methods</journal-title></journal-title-group><issn pub-type="ppub">0166-0934</issn><issn pub-type="epub">1879-0984</issn><publisher><publisher-name>The Authors. Published by Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24530430</article-id><article-id pub-id-type="pmc">7113645</article-id><article-id pub-id-type="publisher-id">S0166-0934(14)00032-9</article-id><article-id pub-id-type="doi">10.1016/j.jviromet.2014.01.022</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Chang</surname><given-names>Hsin-Hou</given-names></name><xref rid="aff0005" ref-type="aff">a</xref><xref rid="aff0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Chen</surname><given-names>Po-Kong</given-names></name><xref rid="aff0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Lin</surname><given-names>Guan-Ling</given-names></name><xref rid="aff0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Wang</surname><given-names>Chun-Jen</given-names></name><xref rid="aff0015" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="aut0025"><name><surname>Liao</surname><given-names>Chih-Hsien</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0030"><name><surname>Hsiao</surname><given-names>Yu-Cheng</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0035"><name><surname>Dong</surname><given-names>Jing-Hua</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0040"><name><surname>Sun</surname><given-names>Der-Shan</given-names></name><email>dssun@mail.tcu.edu.tw</email><email>dershansun@yahoo.com</email><xref rid="aff0005" ref-type="aff">a</xref><xref rid="aff0010" ref-type="aff">b</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff0005"><label>a</label>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan</aff><aff id="aff0010"><label>b</label>Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan</aff><aff id="aff0015"><label>c</label>The Department of Life Sciences, Tzu-Chi University, Hualien, Taiwan</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author at: Department of Molecular Biology and Human Genetics, Tzu-Chi University, No. 701, Section 3, Zhong-Yang Road, Hualien 970, Taiwan. Tel.: +886 3 8565301x2681; fax: +886 3 8561422. <email>dssun@mail.tcu.edu.tw</email><email>dershansun@yahoo.com</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>13</day><month>2</month><year>2014</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>1</day><month>6</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>13</day><month>2</month><year>2014</year></pub-date><volume>201</volume><fpage>1</fpage><lpage>6</lpage><history><date date-type="received"><day>21</day><month>8</month><year>2013</year></date><date date-type="rev-recd"><day>20</day><month>1</month><year>2014</year></date><date date-type="accepted"><day>24</day><month>1</month><year>2014</year></date></history><permissions><copyright-statement>© 2014 The Authors</copyright-statement><copyright-year>2014</copyright-year><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract abstract-type="author-highlights" id="abs0005"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Cell adhesion assay is a simple method to identify the receptor-binding motif of the SARS-CoV spike.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Synthetic peptides can be used to narrow the receptor-binding motif.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">The 436–445 amino acids are the minimum receptor-binding motif of the SARS-CoV spike.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">Cell adhesion assay can be developed as a high throughput system by using a 96-well MTT assay.</p></list-item></list></p></abstract><abstract id="abs0010"><p>Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10–25 residues. Following analyses, 2 peptides spanning the 436–445 and 437–461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV.</p></abstract><kwd-group id="kwd0005"><title>Abbreviations</title><kwd>SARS-CoV, severe acute respiratory syndrome-coronavirus</kwd><kwd>S, spike</kwd><kwd>RBD, receptor-binding domain</kwd><kwd>ACE2, angiotensin-converting enzyme 2</kwd><kwd>RBM, receptor-binding motif</kwd><kwd>MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide</kwd></kwd-group><kwd-group id="kwd0010"><title>Keywords</title><kwd>SARS-CoV</kwd><kwd>Spike protein</kwd><kwd>Vero E6 cells</kwd><kwd>Binding motif</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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