Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein.
Identifieur interne : 001A55 ( PubMed/Corpus ); précédent : 001A54; suivant : 001A56Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein.
Auteurs : Hsin-Hou Chang ; Po-Kong Chen ; Guan-Ling Lin ; Chun-Jen Wang ; Chih-Hsien Liao ; Yu-Cheng Hsiao ; Jing-Hua Dong ; Der-Shan SunSource :
- Journal of virological methods [ 1879-0984 ] ; 2014.
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : Antiviral Agents, Peptides.
- chemical , metabolism : Spike Glycoprotein, Coronavirus.
- physiology : SARS Virus.
- Animals, Binding Sites, Cell Adhesion, Cell Line, Humans, Protein Binding, Virus Attachment.
Abstract
Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10-25 residues. Following analyses, 2 peptides spanning the 436-445 and 437-461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV.
DOI: 10.1016/j.jviromet.2014.01.022
PubMed: 24530430
Links to Exploration step
pubmed:24530430Le document en format XML
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<front><div type="abstract" xml:lang="en">Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10-25 residues. Following analyses, 2 peptides spanning the 436-445 and 437-461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV. </div>
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