Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein
Identifieur interne : 000C89 ( Ncbi/Checkpoint ); précédent : 000C88; suivant : 000C90Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein
Auteurs : Hsin-Hou Chang [Taïwan] ; Po-Kong Chen [Taïwan] ; Guan-Ling Lin [Taïwan] ; Chun-Jen Wang [Taïwan] ; Chih-Hsien Liao [Taïwan] ; Yu-Cheng Hsiao [Taïwan] ; Jing-Hua Dong [Taïwan] ; Der-Shan Sun [Taïwan]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Antiviraux, Peptides.
- métabolisme : Glycoprotéine de spicule des coronavirus.
- physiologie : Virus du SRAS.
- Adhérence cellulaire, Animaux, Attachement viral, Humains, Liaison aux protéines, Lignée cellulaire, Sites de fixation.
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : Antiviral Agents, Peptides.
- chemical , metabolism : Spike Glycoprotein, Coronavirus.
- physiology : SARS Virus.
- Animals, Binding Sites, Cell Adhesion, Cell Line, Humans, Protein Binding, Virus Attachment.
Abstract
Cell adhesion assay is a simple method to identify the receptor-binding motif of the SARS-CoV spike. Synthetic peptides can be used to narrow the receptor-binding motif. The 436–445 amino acids are the minimum receptor-binding motif of the SARS-CoV spike. Cell adhesion assay can be developed as a high throughput system by using a 96-well MTT assay.
Url:
DOI: 10.1016/j.jviromet.2014.01.022
PubMed: 24530430
PubMed Central: 7113645
Affiliations:
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Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Hsiao, Yu Cheng" sort="Hsiao, Yu Cheng" uniqKey="Hsiao Y" first="Yu-Cheng" last="Hsiao">Yu-Cheng Hsiao</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Jing Hua" sort="Dong, Jing Hua" uniqKey="Dong J" first="Jing-Hua" last="Dong">Jing-Hua Dong</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Der Shan" sort="Sun, Der Shan" uniqKey="Sun D" first="Der-Shan" last="Sun">Der-Shan Sun</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Medical Sciences, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Virological Methods</title><idno type="ISSN">0166-0934</idno><idno type="eISSN">1879-0984</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antiviral Agents (isolation & purification)</term><term>Binding Sites</term><term>Cell Adhesion</term><term>Cell Line</term><term>Humans</term><term>Peptides (isolation & purification)</term><term>Protein Binding</term><term>SARS Virus (physiology)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Virus Attachment</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adhérence cellulaire</term><term>Animaux</term><term>Antiviraux (isolement et purification)</term><term>Attachement viral</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Peptides (isolement et purification)</term><term>Sites de fixation</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antiviral Agents</term><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antiviraux</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Binding Sites</term><term>Cell Adhesion</term><term>Cell Line</term><term>Humans</term><term>Protein Binding</term><term>Virus Attachment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adhérence cellulaire</term><term>Animaux</term><term>Attachement viral</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Sites de fixation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Cell adhesion assay is a simple method to identify the receptor-binding motif of the SARS-CoV spike.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Synthetic peptides can be used to narrow the receptor-binding motif.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">The 436–445 amino acids are the minimum receptor-binding motif of the SARS-CoV spike.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">Cell adhesion assay can be developed as a high throughput system by using a 96-well MTT assay.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Babcock, G J" uniqKey="Babcock G">G.J. Babcock</name></author><author><name sortKey="Esshaki, D J" uniqKey="Esshaki D">D.J. Esshaki</name></author><author><name sortKey="Thomas, W D" uniqKey="Thomas W">W.D. Thomas</name></author><author><name sortKey="Ambrosino, D M" uniqKey="Ambrosino D">D.M. Ambrosino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brynda, E" uniqKey="Brynda E">E. Brynda</name></author><author><name sortKey="Pachernik, J" uniqKey="Pachernik J">J. Pachernik</name></author><author><name sortKey="Houska, M" uniqKey="Houska M">M. Houska</name></author><author><name sortKey="Pientka, Z" uniqKey="Pientka Z">Z. Pientka</name></author><author><name sortKey="Dvorak, P" uniqKey="Dvorak P">P. Dvorak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chakraborti, S" uniqKey="Chakraborti S">S. Chakraborti</name></author><author><name sortKey="Prabakaran, P" uniqKey="Prabakaran P">P. 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Luo</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Chang, Hsin Hou" sort="Chang, Hsin Hou" uniqKey="Chang H" first="Hsin-Hou" last="Chang">Hsin-Hou Chang</name></noRegion><name sortKey="Chang, Hsin Hou" sort="Chang, Hsin Hou" uniqKey="Chang H" first="Hsin-Hou" last="Chang">Hsin-Hou Chang</name><name sortKey="Chen, Po Kong" sort="Chen, Po Kong" uniqKey="Chen P" first="Po-Kong" last="Chen">Po-Kong Chen</name><name sortKey="Dong, Jing Hua" sort="Dong, Jing Hua" uniqKey="Dong J" first="Jing-Hua" last="Dong">Jing-Hua Dong</name><name sortKey="Hsiao, Yu Cheng" sort="Hsiao, Yu Cheng" uniqKey="Hsiao Y" first="Yu-Cheng" last="Hsiao">Yu-Cheng Hsiao</name><name sortKey="Liao, Chih Hsien" sort="Liao, Chih Hsien" uniqKey="Liao C" first="Chih-Hsien" last="Liao">Chih-Hsien Liao</name><name sortKey="Lin, Guan Ling" sort="Lin, Guan Ling" uniqKey="Lin G" first="Guan-Ling" last="Lin">Guan-Ling Lin</name><name sortKey="Sun, Der Shan" sort="Sun, Der Shan" uniqKey="Sun D" first="Der-Shan" last="Sun">Der-Shan Sun</name><name sortKey="Sun, Der Shan" sort="Sun, Der Shan" uniqKey="Sun D" first="Der-Shan" last="Sun">Der-Shan Sun</name><name sortKey="Wang, Chun Jen" sort="Wang, Chun Jen" uniqKey="Wang C" first="Chun-Jen" last="Wang">Chun-Jen Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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