Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor†
Identifieur interne : 003A65 ( Main/Exploration ); précédent : 003A64; suivant : 003A66Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor†
Auteurs : L. Michelle Furtado ; Michael ThompsonSource :
- Analyst [ 0003-2654 ] ; 1998.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Oligonucleotide Probes.
- instrumentation : Biosensing Techniques.
- methods : Biosensing Techniques.
- Teeft :
- Acid chemistry, Acoustic, Acoustic energy, Acoustic properties, Acoustic wave devices, Acoustic wave sensor, Ambient, Ambient conditions, Ambient temperature, Ambient temperature conditions, Anal, Base pairing, Biotinylated, Biotinylated oligonucleotide, Boundary condition, Boundary conditions, Buffer solution, Chem, Complementary, Complementary hybridization, Complementary sequence, Complementary strand, Complementary strands, Crystal surface, Device frequency, Device surface, Different responses, Double strand, Drug discovery, Duplex formation, Effective thickness, Electronic Data Processing, Energy dissipation, Flow cell, Flow rate, Frequency decreases, Frequency shifts, Gene sequences, Higher temperature, Higher temperatures, Humans, Hybrid, Hybridization, Hybridization events, Hybridization temperature, Initial change, Interfacial viscosity, Liquid phase, Lower temperatures, Molecular recognitive film, Mutant, Mutation, Neutravidin, Nucleic, Nucleic Acid Hybridization, Nucleic acid, Nucleic acid hybridization, Oligonucleotide, Oligonucleotide hybridization, Oligonucleotides, Other systems, Point Mutation, Present paper, Present work, Pyrimidine, Quartz, Recent times, Resonant frequency, Schematic diagram, Second challenge, Second injection, Sensor, Sequence Analysis, DNA, Series resonance frequency, Series resonance plot, Shear stress, Smaller change, Sound, Substitution.
Abstract
The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid–solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.
Url:
DOI: 10.1039/a804439b
Affiliations:
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Le document en format XML
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<term>Biosensing Techniques (methods)</term>
<term>Electronic Data Processing</term>
<term>Humans</term>
<term>Nucleic Acid Hybridization</term>
<term>Oligonucleotide Probes</term>
<term>Point Mutation</term>
<term>Sequence Analysis, DNA</term>
<term>Sound</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
<term>Mutation ponctuelle</term>
<term>Son (acoustique)</term>
<term>Sondes oligonucléotidiques</term>
<term>Techniques de biocapteur ()</term>
<term>Techniques de biocapteur (instrumentation)</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Oligonucleotide Probes</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term>
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<keywords scheme="Teeft" xml:lang="en"><term>Acid chemistry</term>
<term>Acoustic</term>
<term>Acoustic energy</term>
<term>Acoustic properties</term>
<term>Acoustic wave devices</term>
<term>Acoustic wave sensor</term>
<term>Ambient</term>
<term>Ambient conditions</term>
<term>Ambient temperature</term>
<term>Ambient temperature conditions</term>
<term>Anal</term>
<term>Base pairing</term>
<term>Biotinylated</term>
<term>Biotinylated oligonucleotide</term>
<term>Boundary condition</term>
<term>Boundary conditions</term>
<term>Buffer solution</term>
<term>Chem</term>
<term>Complementary</term>
<term>Complementary hybridization</term>
<term>Complementary sequence</term>
<term>Complementary strand</term>
<term>Complementary strands</term>
<term>Crystal surface</term>
<term>Device frequency</term>
<term>Device surface</term>
<term>Different responses</term>
<term>Double strand</term>
<term>Drug discovery</term>
<term>Duplex formation</term>
<term>Effective thickness</term>
<term>Electronic Data Processing</term>
<term>Energy dissipation</term>
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<term>Flow rate</term>
<term>Frequency decreases</term>
<term>Frequency shifts</term>
<term>Gene sequences</term>
<term>Higher temperature</term>
<term>Higher temperatures</term>
<term>Humans</term>
<term>Hybrid</term>
<term>Hybridization</term>
<term>Hybridization events</term>
<term>Hybridization temperature</term>
<term>Initial change</term>
<term>Interfacial viscosity</term>
<term>Liquid phase</term>
<term>Lower temperatures</term>
<term>Molecular recognitive film</term>
<term>Mutant</term>
<term>Mutation</term>
<term>Neutravidin</term>
<term>Nucleic</term>
<term>Nucleic Acid Hybridization</term>
<term>Nucleic acid</term>
<term>Nucleic acid hybridization</term>
<term>Oligonucleotide</term>
<term>Oligonucleotide hybridization</term>
<term>Oligonucleotides</term>
<term>Other systems</term>
<term>Point Mutation</term>
<term>Present paper</term>
<term>Present work</term>
<term>Pyrimidine</term>
<term>Quartz</term>
<term>Recent times</term>
<term>Resonant frequency</term>
<term>Schematic diagram</term>
<term>Second challenge</term>
<term>Second injection</term>
<term>Sensor</term>
<term>Sequence Analysis, DNA</term>
<term>Series resonance frequency</term>
<term>Series resonance plot</term>
<term>Shear stress</term>
<term>Smaller change</term>
<term>Sound</term>
<term>Substitution</term>
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<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
<term>Mutation ponctuelle</term>
<term>Son (acoustique)</term>
<term>Sondes oligonucléotidiques</term>
<term>Techniques de biocapteur</term>
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<front><div type="abstract">The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid–solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.</div>
</front>
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