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Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor†

Identifieur interne : 003A65 ( Main/Exploration ); précédent : 003A64; suivant : 003A66

Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor†

Auteurs : L. Michelle Furtado ; Michael Thompson

Source :

RBID : ISTEX:5E59A53C9038B6D6C778CB865FFC53AFED06AD04

Descripteurs français

English descriptors

Abstract

The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid–solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.

Url:
DOI: 10.1039/a804439b


Affiliations:


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Le document en format XML

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<term>Electronic Data Processing</term>
<term>Humans</term>
<term>Nucleic Acid Hybridization</term>
<term>Oligonucleotide Probes</term>
<term>Point Mutation</term>
<term>Sequence Analysis, DNA</term>
<term>Sound</term>
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<term>Analyse de séquence d'ADN</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
<term>Mutation ponctuelle</term>
<term>Son (acoustique)</term>
<term>Sondes oligonucléotidiques</term>
<term>Techniques de biocapteur ()</term>
<term>Techniques de biocapteur (instrumentation)</term>
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<term>Oligonucleotide Probes</term>
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<term>Biosensing Techniques</term>
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<term>Biosensing Techniques</term>
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<term>Acid chemistry</term>
<term>Acoustic</term>
<term>Acoustic energy</term>
<term>Acoustic properties</term>
<term>Acoustic wave devices</term>
<term>Acoustic wave sensor</term>
<term>Ambient</term>
<term>Ambient conditions</term>
<term>Ambient temperature</term>
<term>Ambient temperature conditions</term>
<term>Anal</term>
<term>Base pairing</term>
<term>Biotinylated</term>
<term>Biotinylated oligonucleotide</term>
<term>Boundary condition</term>
<term>Boundary conditions</term>
<term>Buffer solution</term>
<term>Chem</term>
<term>Complementary</term>
<term>Complementary hybridization</term>
<term>Complementary sequence</term>
<term>Complementary strand</term>
<term>Complementary strands</term>
<term>Crystal surface</term>
<term>Device frequency</term>
<term>Device surface</term>
<term>Different responses</term>
<term>Double strand</term>
<term>Drug discovery</term>
<term>Duplex formation</term>
<term>Effective thickness</term>
<term>Electronic Data Processing</term>
<term>Energy dissipation</term>
<term>Flow cell</term>
<term>Flow rate</term>
<term>Frequency decreases</term>
<term>Frequency shifts</term>
<term>Gene sequences</term>
<term>Higher temperature</term>
<term>Higher temperatures</term>
<term>Humans</term>
<term>Hybrid</term>
<term>Hybridization</term>
<term>Hybridization events</term>
<term>Hybridization temperature</term>
<term>Initial change</term>
<term>Interfacial viscosity</term>
<term>Liquid phase</term>
<term>Lower temperatures</term>
<term>Molecular recognitive film</term>
<term>Mutant</term>
<term>Mutation</term>
<term>Neutravidin</term>
<term>Nucleic</term>
<term>Nucleic Acid Hybridization</term>
<term>Nucleic acid</term>
<term>Nucleic acid hybridization</term>
<term>Oligonucleotide</term>
<term>Oligonucleotide hybridization</term>
<term>Oligonucleotides</term>
<term>Other systems</term>
<term>Point Mutation</term>
<term>Present paper</term>
<term>Present work</term>
<term>Pyrimidine</term>
<term>Quartz</term>
<term>Recent times</term>
<term>Resonant frequency</term>
<term>Schematic diagram</term>
<term>Second challenge</term>
<term>Second injection</term>
<term>Sensor</term>
<term>Sequence Analysis, DNA</term>
<term>Series resonance frequency</term>
<term>Series resonance plot</term>
<term>Shear stress</term>
<term>Smaller change</term>
<term>Sound</term>
<term>Substitution</term>
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<term>Hybridation d'acides nucléiques</term>
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<term>Son (acoustique)</term>
<term>Sondes oligonucléotidiques</term>
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<div type="abstract">The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid–solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.</div>
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