Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor.
Identifieur interne : 000015 ( Ncbi/Curation ); précédent : 000014; suivant : 000016Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor.
Auteurs : L M Furtado [Canada] ; M. ThompsonSource :
- The Analyst [ 0003-2654 ] ; 1998.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Oligonucleotide Probes.
- instrumentation : Biosensing Techniques.
- methods : Biosensing Techniques.
- Electronic Data Processing, Humans, Nucleic Acid Hybridization, Point Mutation, Sequence Analysis, DNA, Sound.
Abstract
The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid-solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.
DOI: 10.1039/a804439b
PubMed: 10209884
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pubmed:10209884Le document en format XML
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<affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, University of Toronto, Ontario, Canada. bgranozi@alchemy.chem.utoronto.ca</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Chemistry, University of Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
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<author><name sortKey="Thompson, M" sort="Thompson, M" uniqKey="Thompson M" first="M" last="Thompson">M. Thompson</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor.</title>
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<affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, University of Toronto, Ontario, Canada. bgranozi@alchemy.chem.utoronto.ca</nlm:affiliation>
<country xml:lang="fr">Canada</country>
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<author><name sortKey="Thompson, M" sort="Thompson, M" uniqKey="Thompson M" first="M" last="Thompson">M. Thompson</name>
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<series><title level="j">The Analyst</title>
<idno type="ISSN">0003-2654</idno>
<imprint><date when="1998" type="published">1998</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (instrumentation)</term>
<term>Biosensing Techniques (methods)</term>
<term>Electronic Data Processing</term>
<term>Humans</term>
<term>Nucleic Acid Hybridization</term>
<term>Oligonucleotide Probes</term>
<term>Point Mutation</term>
<term>Sequence Analysis, DNA</term>
<term>Sound</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
<term>Mutation ponctuelle</term>
<term>Son (acoustique)</term>
<term>Sondes oligonucléotidiques</term>
<term>Techniques de biocapteur ()</term>
<term>Techniques de biocapteur (instrumentation)</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Oligonucleotide Probes</term>
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<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term>
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<term>Humans</term>
<term>Nucleic Acid Hybridization</term>
<term>Point Mutation</term>
<term>Sequence Analysis, DNA</term>
<term>Sound</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Humains</term>
<term>Hybridation d'acides nucléiques</term>
<term>Mutation ponctuelle</term>
<term>Son (acoustique)</term>
<term>Sondes oligonucléotidiques</term>
<term>Techniques de biocapteur</term>
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<front><div type="abstract" xml:lang="en">The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid-solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.</div>
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