Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor.
Identifieur interne : 002633 ( PubMed/Corpus ); précédent : 002632; suivant : 002634Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor.
Auteurs : L M Furtado ; M. ThompsonSource :
- The Analyst [ 0003-2654 ] ; 1998.
English descriptors
- KwdEn :
- MESH :
- chemical : Oligonucleotide Probes.
- instrumentation : Biosensing Techniques.
- methods : Biosensing Techniques.
- Electronic Data Processing, Humans, Nucleic Acid Hybridization, Point Mutation, Sequence Analysis, DNA, Sound.
Abstract
The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid-solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.
DOI: 10.1039/a804439b
PubMed: 10209884
Links to Exploration step
pubmed:10209884Le document en format XML
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Thompson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:10209884</idno><idno type="pmid">10209884</idno><idno type="doi">10.1039/a804439b</idno><idno type="wicri:Area/PubMed/Corpus">002633</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002633</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor.</title><author><name sortKey="Furtado, L M" sort="Furtado, L M" uniqKey="Furtado L" first="L M" last="Furtado">L M Furtado</name><affiliation><nlm:affiliation>Department of Chemistry, University of Toronto, Ontario, Canada. bgranozi@alchemy.chem.utoronto.ca</nlm:affiliation></affiliation></author><author><name sortKey="Thompson, M" sort="Thompson, M" uniqKey="Thompson M" first="M" last="Thompson">M. Thompson</name></author></analytic><series><title level="j">The Analyst</title><idno type="ISSN">0003-2654</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (instrumentation)</term><term>Biosensing Techniques (methods)</term><term>Electronic Data Processing</term><term>Humans</term><term>Nucleic Acid Hybridization</term><term>Oligonucleotide Probes</term><term>Point Mutation</term><term>Sequence Analysis, DNA</term><term>Sound</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Oligonucleotide Probes</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electronic Data Processing</term><term>Humans</term><term>Nucleic Acid Hybridization</term><term>Point Mutation</term><term>Sequence Analysis, DNA</term><term>Sound</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid-solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10209884</PMID><DateCompleted><Year>1999</Year><Month>04</Month><Day>30</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>22</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0003-2654</ISSN><JournalIssue CitedMedium="Print"><Volume>123</Volume><Issue>10</Issue><PubDate><Year>1998</Year><Month>Oct</Month></PubDate></JournalIssue><Title>The Analyst</Title><ISOAbbreviation>Analyst</ISOAbbreviation></Journal><ArticleTitle>Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor.</ArticleTitle><Pagination><MedlinePgn>1937-45</MedlinePgn></Pagination><Abstract><AbstractText>The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid-solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Furtado</LastName><ForeName>L M</ForeName><Initials>LM</Initials><AffiliationInfo><Affiliation>Department of Chemistry, University of Toronto, Ontario, Canada. bgranozi@alchemy.chem.utoronto.ca</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thompson</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Analyst</MedlineTA><NlmUniqueID>0372652</NlmUniqueID><ISSNLinking>0003-2654</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015345">Oligonucleotide Probes</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015374" MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001330" MajorTopicYN="N">Electronic Data Processing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009693" MajorTopicYN="Y">Nucleic Acid Hybridization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015345" MajorTopicYN="N">Oligonucleotide Probes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017354" MajorTopicYN="N">Point Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="Y">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013016" MajorTopicYN="N">Sound</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1999</Year><Month>4</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1999</Year><Month>4</Month><Day>21</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1999</Year><Month>4</Month><Day>21</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10209884</ArticleId><ArticleId IdType="doi">10.1039/a804439b</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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