Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor.
Identifieur interne : 000015 ( Ncbi/Checkpoint ); précédent : 000014; suivant : 000016Hybridization of complementary strand and single-base mutated oligonucleotides detected with an on-line acoustic wave sensor.
Auteurs : L M Furtado [Canada] ; M. ThompsonSource :
- The Analyst [ 0003-2654 ] ; 1998.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Oligonucleotide Probes.
- instrumentation : Biosensing Techniques.
- methods : Biosensing Techniques.
- Electronic Data Processing, Humans, Nucleic Acid Hybridization, Point Mutation, Sequence Analysis, DNA, Sound.
Abstract
The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid-solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.
DOI: 10.1039/a804439b
PubMed: 10209884
Affiliations:
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pubmed:10209884Le document en format XML
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Thompson</name></author></analytic><series><title level="j">The Analyst</title><idno type="ISSN">0003-2654</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (instrumentation)</term><term>Biosensing Techniques (methods)</term><term>Electronic Data Processing</term><term>Humans</term><term>Nucleic Acid Hybridization</term><term>Oligonucleotide Probes</term><term>Point Mutation</term><term>Sequence Analysis, DNA</term><term>Sound</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Humains</term><term>Hybridation d'acides nucléiques</term><term>Mutation ponctuelle</term><term>Son (acoustique)</term><term>Sondes oligonucléotidiques</term><term>Techniques de biocapteur ()</term><term>Techniques de biocapteur (instrumentation)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Oligonucleotide Probes</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electronic Data Processing</term><term>Humans</term><term>Nucleic Acid Hybridization</term><term>Point Mutation</term><term>Sequence Analysis, DNA</term><term>Sound</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Humains</term><term>Hybridation d'acides nucléiques</term><term>Mutation ponctuelle</term><term>Son (acoustique)</term><term>Sondes oligonucléotidiques</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The hybridization of a biotinylated 25-mer oligonucleotide probe with complementary, non-complementary and single-base mutated 25-mer targets at the liquid-solid (neutravidin-modified) interface of a thickness-shear mode acoustic wave device was studied. The sensor was incorporated into an on-line configuration capable of both variable flow and stop-flow experiments. Under ambient temperature conditions different signals were obtained for the complementary and non-complementary cases. At higher temperatures, the former system exhibits behaviour characteristic of the production of intermediate duplexes which are decomposed by the re-introduction of buffer solution. The use of these conditions allows the distinction of binding events involving a set of single-base mutated 25-mers. Different responses were obtained depending on both the nature of the instigated mismatch in base pairing and on the location of mutation.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Toronto</li></settlement><orgName><li>Université de Toronto</li></orgName></list><tree><noCountry><name sortKey="Thompson, M" sort="Thompson, M" uniqKey="Thompson M" first="M" last="Thompson">M. Thompson</name></noCountry><country name="Canada"><region name="Ontario"><name sortKey="Furtado, L M" sort="Furtado, L M" uniqKey="Furtado L" first="L M" last="Furtado">L M Furtado</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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