MersV1, Main, Exploration, bibRecord, 004A86

Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence

Identifieur interne : 004A86 ( Main/Exploration ); précédent : 004A85; suivant : 004A87

Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence

Auteurs : E. P. Rybicki ; F. L. Hughes

Source :

Journal of general virology [ 0022-1317 ] ; 1990.

RBID : Pascal:91-0180790

Descripteurs français

KwdFr :
- Données de séquences moléculaires, Phylogénie, Plasmides, Poaceae (microbiologie), Réaction de polymérisation en chaîne, Sensibilité et spécificité, Sondes d'ADN, Séquence d'acides aminés, Séquence nucléotidique, Sérotypage, Virus des plantes (), Virus des plantes (génétique), Zea mays (microbiologie).
MESH :
- génétique : Virus des plantes.
- microbiologie : Poaceae, Zea mays.
Pascal (Inist)
- Données de séquences moléculaires, Phylogénie, Plasmides, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Sondes d'ADN, Séquence d'acides aminés, Séquence nucléotidique, Sérotypage, Virus des plantes, Virus streak maïs, Séquence conservée, Phylogenèse, Isolat, Phytopathogène, Séquence nucléotide, Homologie, Spécificité, Clonage moléculaire, Hybridation moléculaire, Méthode Southern, Détection, Réaction chaîne polymérase.

English descriptors

KwdEn :
- Amino Acid Sequence, Base Sequence, Conserved sequence, DNA Probes, Detection, Homology, Isolate, Maize streak virus, Molecular Sequence Data, Molecular cloning, Molecular hybridization, Nucleotide sequence, Phylogeny, Phytopathogen, Plant Viruses (classification), Plant Viruses (genetics), Plasmids, Poaceae (microbiology), Polymerase Chain Reaction, Sensitivity and Specificity, Serotyping, Southern blotting, Specificity, Zea mays (microbiology).
MESH :
- chemical : DNA Probes.
- classification : Plant Viruses.
- genetics : Plant Viruses.
- microbiology : Poaceae, Zea mays.
- Amino Acid Sequence, Base Sequence, Molecular Sequence Data, Phylogeny, Plasmids, Polymerase Chain Reaction, Sensitivity and Specificity, Serotyping.

Abstract

The oligonucleotide primers used for amplifications were 17-mers which contained a number of degeneracies. An approximately 250 base pair fragment was amplified from all geminivirus-infected grass and cereal samples tested. The amplification reaction was specific, working down to a concentration of 50 fg/ml of MSV-specific plasmid-cloned DNA and with a 10^-9 dilution of MSV-infected maize DNA extract

Affiliations:

Le document en format XML

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<term>DNA Probes</term>
<term>Detection</term>
<term>Homology</term>
<term>Isolate</term>
<term>Maize streak virus</term>
<term>Molecular Sequence Data</term>
<term>Molecular cloning</term>
<term>Molecular hybridization</term>
<term>Nucleotide sequence</term>
<term>Phylogeny</term>
<term>Phytopathogen</term>
<term>Plant Viruses (classification)</term>
<term>Plant Viruses (genetics)</term>
<term>Plasmids</term>
<term>Poaceae (microbiology)</term>
<term>Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
<term>Serotyping</term>
<term>Southern blotting</term>
<term>Specificity</term>
<term>Zea mays (microbiology)</term>
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<term>Phylogénie</term>
<term>Plasmides</term>
<term>Poaceae (microbiologie)</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Sondes d'ADN</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Sérotypage</term>
<term>Virus des plantes ()</term>
<term>Virus des plantes (génétique)</term>
<term>Zea mays (microbiologie)</term>
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<term>Base Sequence</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Plasmids</term>
<term>Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
<term>Serotyping</term>
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<term>Plasmides</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Sondes d'ADN</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Sérotypage</term>
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<term>Virus streak maïs</term>
<term>Séquence conservée</term>
<term>Phylogenèse</term>
<term>Isolat</term>
<term>Phytopathogène</term>
<term>Séquence nucléotide</term>
<term>Homologie</term>
<term>Spécificité</term>
<term>Clonage moléculaire</term>
<term>Hybridation moléculaire</term>
<term>Méthode Southern</term>
<term>Détection</term>
<term>Réaction chaîne polymérase</term>
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<front><div type="abstract" xml:lang="en">The oligonucleotide primers used for amplifications were 17-mers which contained a number of degeneracies. An approximately 250 base pair fragment was amplified from all geminivirus-infected grass and cereal samples tested. The amplification reaction was specific, working down to a concentration of 50 fg/ml of MSV-specific plasmid-cloned DNA and with a 10<sup>-9</sup>
 dilution of MSV-infected maize DNA extract</div>
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Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence

Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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