Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence.
Identifieur interne : 004A78 ( Main/Merge ); précédent : 004A77; suivant : 004A79Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence.
Auteurs : E P Rybicki [Afrique du Sud] ; F L HughesSource :
- The Journal of general virology [ 0022-1317 ] ; 1990.
Descripteurs français
- KwdFr :
- Données de séquences moléculaires, Phylogénie, Plasmides, Poaceae (microbiologie), Réaction de polymérisation en chaîne, Sensibilité et spécificité, Sondes d'ADN, Séquence d'acides aminés, Séquence nucléotidique, Sérotypage, Virus des plantes (), Virus des plantes (génétique), Zea mays (microbiologie).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : DNA Probes.
- classification : Plant Viruses.
- genetics : Plant Viruses.
- microbiology : Poaceae, Zea mays.
- Amino Acid Sequence, Base Sequence, Molecular Sequence Data, Phylogeny, Plasmids, Polymerase Chain Reaction, Sensitivity and Specificity, Serotyping.
Abstract
The application of the polymerase chain reaction DNA amplification technique to the detection and typing of isolates of maize streak virus (MSV) and other related geminiviruses of grasses is described. The oligonucleotide primers used for amplification were 17-mers which contained a number of degeneracies. An approximately 250 base pair fragment was amplified from all geminivirus-infected grass and cereal samples tested. The amplification reaction was specific, working down to a concentration of 50 fg/ml of MSV-specific plasmid-cloned DNA and with a 10(-9) dilution of MSV-infected maize DNA extract. DNA could also be amplified from distantly related geminiviruses, including two different sugarcane viruses, digitaria streak virus and another as yet uncharacterized virus of a Panicum sp. Amplified DNA from a Mauritian sugarcane isolate (SSV-M) was cloned and sequenced. Sequence comparison and phylogenetic analysis showed that this sequence differed sufficiently from the analogous region of other geminiviruses for SSV-M to be considered a distinct virus. The use of the polymerase chain reaction for the amplification of gemini- and other virus genomes or genomic fragments for typing, mapping, phylogenetic analysis and taxonomy is discussed.
DOI: 10.1099/0022-1317-71-11-2519
PubMed: 2254750
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amplification of a conserved viral sequence.</title><author><name sortKey="Rybicki, E P" sort="Rybicki, E P" uniqKey="Rybicki E" first="E P" last="Rybicki">E P Rybicki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Cape Town, Rondebosch, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Microbiology, University of Cape Town, Rondebosch</wicri:regionArea><wicri:noRegion>Rondebosch</wicri:noRegion></affiliation></author><author><name sortKey="Hughes, F L" sort="Hughes, F L" uniqKey="Hughes F" first="F L" last="Hughes">F L Hughes</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Base 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qualifier="classification" xml:lang="en"><term>Plant Viruses</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Poaceae</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Poaceae</term><term>Zea mays</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Plasmids</term><term>Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Serotyping</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Phylogénie</term><term>Plasmides</term><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term><term>Sondes d'ADN</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Sérotypage</term><term>Virus des plantes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The application of the polymerase chain reaction DNA amplification technique to the detection and typing of isolates of maize streak virus (MSV) and other related geminiviruses of grasses is described. The oligonucleotide primers used for amplification were 17-mers which contained a number of degeneracies. An approximately 250 base pair fragment was amplified from all geminivirus-infected grass and cereal samples tested. The amplification reaction was specific, working down to a concentration of 50 fg/ml of MSV-specific plasmid-cloned DNA and with a 10(-9) dilution of MSV-infected maize DNA extract. DNA could also be amplified from distantly related geminiviruses, including two different sugarcane viruses, digitaria streak virus and another as yet uncharacterized virus of a Panicum sp. Amplified DNA from a Mauritian sugarcane isolate (SSV-M) was cloned and sequenced. Sequence comparison and phylogenetic analysis showed that this sequence differed sufficiently from the analogous region of other geminiviruses for SSV-M to be considered a distinct virus. The use of the polymerase chain reaction for the amplification of gemini- and other virus genomes or genomic fragments for typing, mapping, phylogenetic analysis and taxonomy is discussed.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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