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Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence

Identifieur interne : 000116 ( PascalFrancis/Corpus ); précédent : 000115; suivant : 000117

Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence

Auteurs : E. P. Rybicki ; F. L. Hughes

Source :

RBID : Pascal:91-0180790

Descripteurs français

English descriptors

Abstract

The oligonucleotide primers used for amplifications were 17-mers which contained a number of degeneracies. An approximately 250 base pair fragment was amplified from all geminivirus-infected grass and cereal samples tested. The amplification reaction was specific, working down to a concentration of 50 fg/ml of MSV-specific plasmid-cloned DNA and with a 10-9 dilution of MSV-infected maize DNA extract

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 0022-1317
A02 01      @0 JGVIAY
A03   1    @0 J. Gen. Virol.
A05       @2 71
A06       @2 11
A08 01  1  ENG  @1 Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence
A11 01  1    @1 RYBICKI (E. P.)
A11 02  1    @1 HUGHES (F. L.)
A14 01      @1 Univ. Cape Town, dep. microbiology @2 Rondebosch 7700 @3 ZAF @Z A11011000
A20       @1 2519-2526
A21       @1 1990
A23 01      @0 ENG
A43 01      @1 INIST @2 13533 @3 354000015493370040/NUM
A44       @0 0000
A45       @0 p.
A47 01  1    @0 91-0180790
A60       @1 P
A61       @0 A
A64   1    @0 Journal of general virology
A66 01      @0 GBR
C01 01    ENG  @0 The oligonucleotide primers used for amplifications were 17-mers which contained a number of degeneracies. An approximately 250 base pair fragment was amplified from all geminivirus-infected grass and cereal samples tested. The amplification reaction was specific, working down to a concentration of 50 fg/ml of MSV-specific plasmid-cloned DNA and with a 10-9 dilution of MSV-infected maize DNA extract
C02 01  X    @0 002A05C05
C03 01  X  FRE  @0 Virus streak maïs
C03 03  X  FRE  @0 Séquence conservée
C03 07  X  FRE  @0 Phylogenèse
C03 08  X  FRE  @0 Isolat
C03 09  X  FRE  @0 Phytopathogène
C03 10  X  FRE  @0 Séquence nucléotide
C03 11  X  FRE  @0 Homologie
C03 12  X  FRE  @0 Spécificité
C03 13  X  FRE  @0 Clonage moléculaire
C03 14  X  FRE  @0 Hybridation moléculaire
C03 15  X  FRE  @0 Méthode Southern
C03 16  X  FRE  @0 Détection
C03 17  X  FRE  @0 Réaction chaîne polymérase @4 INC
C03 01  X  ENG  @0 Maize streak virus
C03 03  X  ENG  @0 Conserved sequence
C03 07  X  ENG  @0 Phylogeny
C03 08  X  ENG  @0 Isolate
C03 09  X  ENG  @0 Phytopathogen
C03 10  X  ENG  @0 Nucleotide sequence
C03 11  X  ENG  @0 Homology
C03 12  X  ENG  @0 Specificity
C03 13  X  ENG  @0 Molecular cloning
C03 14  X  ENG  @0 Molecular hybridization
C03 15  X  ENG  @0 Southern blotting
C03 16  X  ENG  @0 Detection
C03 01  X  SPA  @0 Maize streak virus
C03 03  X  SPA  @0 Secuencia conservada
C03 07  X  SPA  @0 Filogénesis
C03 08  X  SPA  @0 Aislado
C03 09  X  SPA  @0 Fitopatógeno
C03 10  X  SPA  @0 Secuencia nucleótido
C03 11  X  SPA  @0 Homología
C03 12  X  SPA  @0 Especificidad
C03 13  X  SPA  @0 Clonación molecular
C03 14  X  SPA  @0 Hibridación molecular
C03 16  X  SPA  @0 Detección
C07 01  X  FRE  @0 Geminivirus
C07 02  X  FRE  @0 Virus
C07 03  X  ENG  @0 Geminivirus
C07 04  X  ENG  @0 Virus
C07 05  X  SPA  @0 Geminivirus
C07 06  X  SPA  @0 Virus
N21       @1 048
N82       @1 NIM

Format Inist (serveur)

NO : PASCAL 91-0180790 INIST
ET : Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence
AU : RYBICKI (E. P.); HUGHES (F. L.)
AF : Univ. Cape Town, dep. microbiology/Rondebosch 7700/Afrique du Sud (A11011000)
DT : Publication en série; Niveau analytique
SO : Journal of general virology; ISSN 0022-1317; Coden JGVIAY; Royaume-Uni; Da. 1990; Vol. 71; No. 11; Pp. 2519-2526; Bibl. p.
LA : Anglais
EA : The oligonucleotide primers used for amplifications were 17-mers which contained a number of degeneracies. An approximately 250 base pair fragment was amplified from all geminivirus-infected grass and cereal samples tested. The amplification reaction was specific, working down to a concentration of 50 fg/ml of MSV-specific plasmid-cloned DNA and with a 10-9 dilution of MSV-infected maize DNA extract
CC : 002A05C05
FD : Virus streak maïs; Séquence conservée; Phylogenèse; Isolat; Phytopathogène; Séquence nucléotide; Homologie; Spécificité; Clonage moléculaire; Hybridation moléculaire; Méthode Southern; Détection; Réaction chaîne polymérase
FG : Geminivirus; Virus
ED : Maize streak virus; Conserved sequence; Phylogeny; Isolate; Phytopathogen; Nucleotide sequence; Homology; Specificity; Molecular cloning; Molecular hybridization; Southern blotting; Detection
EG : Geminivirus; Virus
SD : Maize streak virus; Secuencia conservada; Filogénesis; Aislado; Fitopatógeno; Secuencia nucleótido; Homología; Especificidad; Clonación molecular; Hibridación molecular; Detección
LO : INIST-13533
ID : 91-0180790

Links to Exploration step

Pascal:91-0180790

Le document en format XML

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<ET>Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence</ET>
<AU>RYBICKI (E. P.); HUGHES (F. L.)</AU>
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<sup>-9</sup>
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