Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence
Identifieur interne : 000117 ( PascalFrancis/Checkpoint ); précédent : 000116; suivant : 000118Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence
Auteurs : E. P. Rybicki ; F. L. HughesSource :
- Journal of general virology [ 0022-1317 ] ; 1990.
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- Pascal (Inist)
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Abstract
The oligonucleotide primers used for amplifications were 17-mers which contained a number of degeneracies. An approximately 250 base pair fragment was amplified from all geminivirus-infected grass and cereal samples tested. The amplification reaction was specific, working down to a concentration of 50 fg/ml of MSV-specific plasmid-cloned DNA and with a 10-9 dilution of MSV-infected maize DNA extract
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Hughes</name></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">91-0180790</idno><date when="1990">1990</date><idno type="stanalyst">PASCAL 91-0180790 INIST</idno><idno type="RBID">Pascal:91-0180790</idno><idno type="wicri:Area/PascalFrancis/Corpus">000116</idno><idno type="wicri:Area/PascalFrancis/Curation">000098</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000117</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000117</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence</title><author><name sortKey="Rybicki, E P" sort="Rybicki, E P" uniqKey="Rybicki E" first="E. P." last="Rybicki">E. P. 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L." last="Hughes">F. L. Hughes</name><name sortKey="Rybicki, E P" sort="Rybicki, E P" uniqKey="Rybicki E" first="E. P." last="Rybicki">E. P. Rybicki</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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