Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of tetrahydrodipicolinate‐N‐succinyltransferase (Rv1201c) from Mycobacterium tuberculosis
Identifieur interne : 000430 ( Istex/Curation ); précédent : 000429; suivant : 000431Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of tetrahydrodipicolinate‐N‐succinyltransferase (Rv1201c) from Mycobacterium tuberculosis
Auteurs : Linda Schuldt [Allemagne] ; Simone Weyand [Allemagne] ; Georgia Kefala [Allemagne] ; Manfred S. Weiss [Allemagne]Source :
- Acta Crystallographica Section F [ 1744-3091 ] ; 2008-09-01.
English descriptors
- Teeft :
- Acta, Acta cryst, Amino acid, Asymmetric unit, Cell pellet, Coli, Cryst, Crystallization, Cubic crystal, Cubic space group, Dapd, Different organisms, Diffraction data collection, Embl hamburg outstation, Enzyme dapd, Escherichia coli, Icosahedral symmetry, Initial crystallization experiments, Kefala, Molecular weight, Mycobacterium tuberculosis, Optical resolution, Peak fractions, Processing parameters, Program molrep, Protein solution, Reservoir solution, Room temperature, Single crystal, Sodium cacodylate, Space group, Space groups, Unpublished results, Vefold axes, Weiss.
Abstract
Tetrahydrodipicolinate‐N‐succinyltransferase from Mycobacterium tuberculosis (DapD, Rv1201c) has been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in the cubic space group I23 or I213. Preliminary diffraction data analysis indicates the presence of five molecules per asymmetric unit. Furthermore, the data exhibit icosahedral point‐group symmetry. One possible explanation for this is that the enzyme assembles into a 60‐mer exhibiting 235 point‐group symmetry and crystallizes as such in space group I23. In this case, the combination of crystallographic and noncrystallographic symmetry elements results in an arrangement of the icosahedrons in the cubic crystal with one pentamer in the asymmetric unit. Another explanation is that the packing of the molecules itself mimics icosahedral symmetry. In this case both space groups I23 and I213 would be possible.
Url:
DOI: 10.1107/S1744309108026559
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Weiss</name><affiliation wicri:level="1"><mods:affiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:763DF7FF09536B11C0BD35F310A49F510985B436</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1107/S1744309108026559</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-QG3MVSTQ-6/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000430</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000430</idno><idno type="wicri:Area/Istex/Curation">000430</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of tetrahydrodipicolinate‐N‐succinyltransferase (Rv1201c) from Mycobacterium tuberculosis</title><author><name sortKey="Schuldt, Linda" sort="Schuldt, Linda" uniqKey="Schuldt L" first="Linda" last="Schuldt">Linda Schuldt</name><affiliation wicri:level="1"><mods:affiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg</wicri:regionArea></affiliation></author><author><name sortKey="Weyand, Simone" sort="Weyand, Simone" uniqKey="Weyand S" first="Simone" last="Weyand">Simone Weyand</name><affiliation wicri:level="1"><mods:affiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg</wicri:regionArea></affiliation></author><author><name sortKey="Kefala, Georgia" sort="Kefala, Georgia" uniqKey="Kefala G" first="Georgia" last="Kefala">Georgia Kefala</name><affiliation wicri:level="1"><mods:affiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg</wicri:regionArea></affiliation></author><author><name sortKey="Weiss, Manfred S" sort="Weiss, Manfred S" uniqKey="Weiss M" first="Manfred S." last="Weiss">Manfred S. Weiss</name><affiliation wicri:level="1"><mods:affiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Acta Crystallographica Section F</title><title level="j" type="alt">ACTA CRYSTALLOGRAPHICA F</title><idno type="ISSN">1744-3091</idno><idno type="eISSN">1744-3091</idno><imprint><biblScope unit="vol">64</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="863">863</biblScope><biblScope unit="page" to="866">866</biblScope><publisher>International Union of Crystallography</publisher><pubPlace>5 Abbey Square, Chester, Cheshire CH1 2HU, England</pubPlace><date type="published" when="2008-09-01">2008-09-01</date></imprint><idno type="ISSN">1744-3091</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1744-3091</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acta</term><term>Acta cryst</term><term>Amino acid</term><term>Asymmetric unit</term><term>Cell pellet</term><term>Coli</term><term>Cryst</term><term>Crystallization</term><term>Cubic crystal</term><term>Cubic space group</term><term>Dapd</term><term>Different organisms</term><term>Diffraction data collection</term><term>Embl hamburg outstation</term><term>Enzyme dapd</term><term>Escherichia coli</term><term>Icosahedral symmetry</term><term>Initial crystallization experiments</term><term>Kefala</term><term>Molecular weight</term><term>Mycobacterium tuberculosis</term><term>Optical resolution</term><term>Peak fractions</term><term>Processing parameters</term><term>Program molrep</term><term>Protein solution</term><term>Reservoir solution</term><term>Room temperature</term><term>Single crystal</term><term>Sodium cacodylate</term><term>Space group</term><term>Space groups</term><term>Unpublished results</term><term>Vefold axes</term><term>Weiss</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tetrahydrodipicolinate‐N‐succinyltransferase from Mycobacterium tuberculosis (DapD, Rv1201c) has been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in the cubic space group I23 or I213. Preliminary diffraction data analysis indicates the presence of five molecules per asymmetric unit. Furthermore, the data exhibit icosahedral point‐group symmetry. One possible explanation for this is that the enzyme assembles into a 60‐mer exhibiting 235 point‐group symmetry and crystallizes as such in space group I23. In this case, the combination of crystallographic and noncrystallographic symmetry elements results in an arrangement of the icosahedrons in the cubic crystal with one pentamer in the asymmetric unit. Another explanation is that the packing of the molecules itself mimics icosahedral symmetry. In this case both space groups I23 and I213 would be possible.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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