Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of tetrahydrodipicolinate‐N‐succinyltransferase (Rv1201c) from Mycobacterium tuberculosis
Identifieur interne : 000430 ( Istex/Corpus ); précédent : 000429; suivant : 000431Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of tetrahydrodipicolinate‐N‐succinyltransferase (Rv1201c) from Mycobacterium tuberculosis
Auteurs : Linda Schuldt ; Simone Weyand ; Georgia Kefala ; Manfred S. WeissSource :
- Acta Crystallographica Section F [ 1744-3091 ] ; 2008-09-01.
English descriptors
- Teeft :
- Acta, Acta cryst, Amino acid, Asymmetric unit, Cell pellet, Coli, Cryst, Crystallization, Cubic crystal, Cubic space group, Dapd, Different organisms, Diffraction data collection, Embl hamburg outstation, Enzyme dapd, Escherichia coli, Icosahedral symmetry, Initial crystallization experiments, Kefala, Molecular weight, Mycobacterium tuberculosis, Optical resolution, Peak fractions, Processing parameters, Program molrep, Protein solution, Reservoir solution, Room temperature, Single crystal, Sodium cacodylate, Space group, Space groups, Unpublished results, Vefold axes, Weiss.
Abstract
Tetrahydrodipicolinate‐N‐succinyltransferase from Mycobacterium tuberculosis (DapD, Rv1201c) has been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in the cubic space group I23 or I213. Preliminary diffraction data analysis indicates the presence of five molecules per asymmetric unit. Furthermore, the data exhibit icosahedral point‐group symmetry. One possible explanation for this is that the enzyme assembles into a 60‐mer exhibiting 235 point‐group symmetry and crystallizes as such in space group I23. In this case, the combination of crystallographic and noncrystallographic symmetry elements results in an arrangement of the icosahedrons in the cubic crystal with one pentamer in the asymmetric unit. Another explanation is that the packing of the molecules itself mimics icosahedral symmetry. In this case both space groups I23 and I213 would be possible.
Url:
DOI: 10.1107/S1744309108026559
Links to Exploration step
ISTEX:763DF7FF09536B11C0BD35F310A49F510985B436Le document en format XML
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2005</json:string><json:string>Vyas et al., 2008</json:string><json:string>de Marco et al., 2007</json:string><json:string>Parish & Stoker, 2002</json:string><json:string>Nguyen et al., 2008</json:string><json:string>Otwinowski & Minor, 1997</json:string><json:string>DapD, Rv1201c</json:string><json:string>Weiss, 2001</json:string><json:string>Kefala, Janowski et al., 2005</json:string><json:string>Kefala & Weiss, 2006, 2008</json:string><json:string>Mueller-Dieckmann, 2006</json:string><json:string>Rv1201c</json:string><json:string>Weyand et al., 2006</json:string><json:string>Cole et al., 1998a,b</json:string><json:string>Beaman et al., 1997, 1998, 2002</json:string><json:string>Hahn, 2002</json:string><json:string>Snider et al., 1994</json:string><json:string>Berges et al., 1986</json:string><json:string>Kefala, Perry et al., 2005</json:string><json:string>Looman et al., 1987</json:string><json:string>Vaguine et al., 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In this case both space groups <hi rend="italic">I</hi>23 and <hi rend="italic">I</hi>2<hi rend="subscript">1</hi>3 would be possible.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="k1">tetrahydrodipicolinate‐<hi rend="italic">N</hi>‐succinyltransferase</term><term xml:id="k2"><hi rend="italic">Mycobacterium tuberculosis</hi></term><term xml:id="k3">DapD</term></keywords><keywords rend="tocHeading1"><term>crystallization communications</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-QG3MVSTQ-6/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>International Union of Crystallography</publisherName><publisherLoc>5 Abbey Square, Chester, Cheshire CH1 2HU, England</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1744-3091</doi><issn type="print">1744-3091</issn><issn type="electronic">1744-3091</issn><idGroup><id type="product" value="AYF2"></id></idGroup><titleGroup><title type="main" sort="ACTA CRYSTALLOGRAPHICA F">Acta Crystallographica Section F</title><title type="short">Acta Cryst. F</title></titleGroup></publicationMeta><publicationMeta level="part" position="09009"><doi origin="wiley">10.1111/ayf.2008.64.issue-9</doi><numberingGroup><numbering type="journalVolume" number="64">64</numbering><numbering type="journalIssue" number="9">9</numbering></numberingGroup><coverDate startDate="2008-09-01">September 2008</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="24" status="forIssue"><doi origin="wiley">10.1107/S1744309108026559</doi><idGroup><id type="unit" value="AYF2HC5060"></id><id type="society" value="hc5060"></id></idGroup><titleGroup><title type="tocHeading1">crystallization communications</title></titleGroup><copyright>International Union of Crystallography, 2008</copyright><eventGroup><event type="firstOnline" date="2008-09-29"></event><event type="publishedOnlineFinalForm" date="2008-09-29"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:Header result:Header" date="2010-02-28"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-14"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="863">863</numbering><numbering type="pageLast" number="866">866</numbering></numberingGroup><correspondenceTo>Manfred S. Weiss, e‐mail: <email normalForm="msweiss@embl-hamburg.de">msweiss@embl‐hamburg.de</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:AYF2.AYF2hc5060.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 11 July 2008, accepted 18 August 2008</unparsedEditorialHistory><countGroup><count type="figureTotal" number="0"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="wordTotal" number="0"></count><count type="referenceTotal" number="0"></count><count type="linksCrossRef" number="0"></count><count type="linksPubMed" number="0"></count></countGroup><titleGroup><title type="main">Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of tetrahydrodipicolinate‐<i>N</i>‐succinyltransferase (Rv1201c) from <i>Mycobacterium tuberculosis</i></title><title type="short">Tetrahydrodipicolinate‐<i>N</i>‐succinyltransferase</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Linda</givenNames><familyName>Schuldt</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>Simone</givenNames><familyName>Weyand</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Georgia</givenNames><familyName>Kefala</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>Manfred S.</givenNames><familyName>Weiss</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="DE"><unparsedAffiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">tetrahydrodipicolinate‐<i>N</i>‐succinyltransferase</keyword><keyword xml:id="k2"><i>Mycobacterium tuberculosis</i></keyword><keyword xml:id="k3">DapD</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>Tetrahydrodipicolinate‐<i>N</i>‐succinyltransferase from <i>Mycobacterium tuberculosis</i> (DapD, Rv1201c) has been cloned, heterologously expressed in <i>Escherichia coli</i>, purified using standard chromatographic techniques and crystallized in the cubic space group <i>I</i>23 or <i>I</i>2<sub>1</sub>3. Preliminary diffraction data analysis indicates the presence of five molecules per asymmetric unit. Furthermore, the data exhibit icosahedral point‐group symmetry. One possible explanation for this is that the enzyme assembles into a 60‐mer exhibiting 235 point‐group symmetry and crystallizes as such in space group <i>I</i>23. In this case, the combination of crystallographic and noncrystallographic symmetry elements results in an arrangement of the icosahedrons in the cubic crystal with one pentamer in the asymmetric unit. Another explanation is that the packing of the molecules itself mimics icosahedral symmetry. In this case both space groups <i>I</i>23 and <i>I</i>2<sub>1</sub>3 would be possible.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of tetrahydrodipicolinate‐N‐succinyltransferase (Rv1201c) from Mycobacterium tuberculosis</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Tetrahydrodipicolinate‐N‐succinyltransferase</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of tetrahydrodipicolinate‐N‐succinyltransferase (Rv1201c) from Mycobacterium tuberculosis</title></titleInfo><name type="personal"><namePart type="given">Linda</namePart><namePart type="family">Schuldt</namePart><affiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Simone</namePart><namePart type="family">Weyand</namePart><affiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Georgia</namePart><namePart type="family">Kefala</namePart><affiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Manfred S.</namePart><namePart type="family">Weiss</namePart><affiliation>EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D‐22603 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>International Union of Crystallography</publisher><place><placeTerm type="text">5 Abbey Square, Chester, Cheshire CH1 2HU, England</placeTerm></place><dateIssued encoding="w3cdtf">2008-09-01</dateIssued><edition>Received 11 July 2008, accepted 18 August 2008</edition><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">0</extent><extent unit="tables">0</extent><extent unit="formulas">0</extent><extent unit="references">0</extent><extent unit="linksCrossRef">0</extent><extent unit="words">0</extent></physicalDescription><abstract lang="en">Tetrahydrodipicolinate‐N‐succinyltransferase from Mycobacterium tuberculosis (DapD, Rv1201c) has been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in the cubic space group I23 or I213. Preliminary diffraction data analysis indicates the presence of five molecules per asymmetric unit. Furthermore, the data exhibit icosahedral point‐group symmetry. One possible explanation for this is that the enzyme assembles into a 60‐mer exhibiting 235 point‐group symmetry and crystallizes as such in space group I23. In this case, the combination of crystallographic and noncrystallographic symmetry elements results in an arrangement of the icosahedrons in the cubic crystal with one pentamer in the asymmetric unit. Another explanation is that the packing of the molecules itself mimics icosahedral symmetry. In this case both space groups I23 and I213 would be possible.</abstract><subject lang="en"><genre>keywords</genre><topic>tetrahydrodipicolinate‐N‐succinyltransferase</topic><topic>Mycobacterium tuberculosis</topic><topic>DapD</topic></subject><relatedItem type="host"><titleInfo><title>Acta Crystallographica Section F</title></titleInfo><titleInfo type="abbreviated"><title>Acta Cryst. F</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">1744-3091</identifier><identifier type="eISSN">1744-3091</identifier><identifier type="DOI">10.1111/(ISSN)1744-3091</identifier><identifier type="PublisherID">AYF2</identifier><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>64</number></detail><detail type="issue"><caption>no.</caption><number>9</number></detail><extent unit="pages"><start>863</start><end>866</end></extent></part></relatedItem><identifier type="istex">763DF7FF09536B11C0BD35F310A49F510985B436</identifier><identifier type="ark">ark:/67375/WNG-QG3MVSTQ-6</identifier><identifier type="DOI">10.1107/S1744309108026559</identifier><identifier type="ArticleID">AYF2HC5060</identifier><accessCondition type="use and reproduction" contentType="copyright">International Union of Crystallography, 2008</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-13</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-QG3MVSTQ-6/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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