Genetic diagnosis of Rehmannia species micropropagated by tip tissue culture and an F1 hybrid by RAPD analysis
Identifieur interne : 000429 ( Istex/Curation ); précédent : 000428; suivant : 000430Genetic diagnosis of Rehmannia species micropropagated by tip tissue culture and an F1 hybrid by RAPD analysis
Auteurs : M. Hatano [Japon] ; R. Nakai [Japon] ; F. Kawanishi2 [Japon] ; K. Kedo2 [Japon] ; Y. Shoyama1 [Japon]Source :
- Plant Breeding [ 0179-9541 ] ; 1997-12.
English descriptors
- Teeft :
- Clonal, Clonal multiplication, Clonal propagation, Genetic analysis, Genetic distance, Genetic distances, Genetic homogeneity, Genetie homogeneity, Glulinosa, Glutinosa, Hybrid, Hybrid strain, Iridoid glycoside contents, Kyushu university, Large amount, Medicinal plants, Micropropagation, Parental, Parental plants, Parental species, Pharmaceutical sciences, Plania mediea, Planl cell, Plant cell, Planta mediea, Plantlet, Plantlets regenerated, Primer, Purpurea, Purpurea makino, Qualitative analysis, Random primers, Rapd, Rapd analysis, Rapd markers, Regenerated, Regenerated plantlets, Rehmannia, Rehmannia glulinosa, Rehmannia species, Root division, Sample material, Secondary metabolites, Shoyama, Takeda chemical industries, Tissue culture, Tuberous roots, Typical bands, Virus infection.
Abstract
Shoot formation was induced from the tip tissue of Rehmannia glutinosa f. hueichingensis on hormone‐free Murashige‐Skoog medium within 4 weeks of culture. Shoots were propagated on Murashige‐Skoog medium supplemented with l.0mg/l benzy0ladenine for 4 weeks. Propagated shoots rooted on the hormone‐free Murashige‐Skoog medium during 4 weeks of culture. Total DNA was extracted from the leaves of a F1 hybrid and its parents. R. glulinosa f. huekliingensis and R. glutinosa var, purpurea. Analysis of random‐amplified polymorphic DNA (RAPD) using 10 arbitrary oligonucleotide 10‐mers. showed the genetic homogeneity ofthe above three species. The F1 hybrid was genetically intermediate between both parental plants, compared with the genetic distance between the F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between the F1 hybrid, obtained from the crossing clearly showed that parts of the bands of both parents. R. glutinosa f. hueichingensis and R. glutinosa var. purpurea, were introduced into the F1 hybrid.
Url:
DOI: 10.1111/j.1439-0523.1997.tb02195.x
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Nakai</name><affiliation wicri:level="1"><mods:affiliation>Faculty of Pharmaceutical Sciences, Kyushu University, Higashiku, Fukuoka 812, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Pharmaceutical Sciences, Kyushu University, Higashiku, Fukuoka 812</wicri:regionArea></affiliation></author><author><name sortKey="Kawanishi2, F" sort="Kawanishi2, F" uniqKey="Kawanishi2 F" first="F." last="Kawanishi2">F. Kawanishi2</name><affiliation wicri:level="1"><mods:affiliation>Fukuchiyama Experitnental Farm, Takeda Chemical Industries Ltd, 4428‐2 Nagata, Fukuchiyama 620‐1, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Fukuchiyama Experitnental Farm, Takeda Chemical Industries Ltd, 4428‐2 Nagata, Fukuchiyama 620‐1</wicri:regionArea></affiliation></author><author><name sortKey="Kedo2, K" sort="Kedo2, K" uniqKey="Kedo2 K" first="K." last="Kedo2">K. 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Shoots were propagated on Murashige‐Skoog medium supplemented with l.0mg/l benzy0ladenine for 4 weeks. Propagated shoots rooted on the hormone‐free Murashige‐Skoog medium during 4 weeks of culture. Total DNA was extracted from the leaves of a F1 hybrid and its parents. R. glulinosa f. huekliingensis and R. glutinosa var, purpurea. Analysis of random‐amplified polymorphic DNA (RAPD) using 10 arbitrary oligonucleotide 10‐mers. showed the genetic homogeneity ofthe above three species. The F1 hybrid was genetically intermediate between both parental plants, compared with the genetic distance between the F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between the F1 hybrid, obtained from the crossing clearly showed that parts of the bands of both parents. R. glutinosa f. hueichingensis and R. glutinosa var. purpurea, were introduced into the F1 hybrid.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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