Collision‐induced dissociation of oligonucleotide anions fully modified at the 2'‐position of the ribose: 2'‐F/‐H and 2'‐F/‐H/‐OMe mix‐mers
Identifieur interne : 000129 ( Istex/Curation ); précédent : 000128; suivant : 000130Collision‐induced dissociation of oligonucleotide anions fully modified at the 2'‐position of the ribose: 2'‐F/‐H and 2'‐F/‐H/‐OMe mix‐mers
Auteurs : Yang Gao ; Scott A. Mcluckey [États-Unis]Source :
- Journal of Mass Spectrometry [ 1076-5174 ] ; 2012-03.
Abstract
Gas‐phase dissociation of various 2'‐position modified oligonucleotide anions has been studied as a function of precursor ion charge state using ion trap and low energy beam‐type collision‐induced dissociation (CID). For a completely 2'‐O‐methyl modified 6‐mer, all possible dissociation channels along the phosphodiester linkage, generating complementary (a‐B)/w‐, b/x‐, c/y‐, d/z‐ion series, were observed with no single dominant type of dissociation pathway. Full sequence information was generated from each charge state via ion trap CID. More sequential fragmentation was noted under beam‐type CID conditions. Comparison with model DNA, in which all 2'‐OH groups are converted to 2'‐H, and RNA anions suggests that the 2'‐OMe substitution stabilizes the phosphodiester linkage with respect to fragmentation relative to both DNA and RNA oligomers. For modified mix‐mer anions, comprised of DNA nucleotides and 2'‐F substituted nucleotides or a mixture of DNA nucleotides and 2'‐O‐methyl (2'‐OMe) and 2'‐F substituted nucleotides, 3'‐side backbone cleavage was found to be inhibited by the 2'‐OMe or 2'‐F modification on the nucleotides under ion trap CID conditions. Thus, the sequence information was limited to the a‐Base/w‐fragments from the cleavage of the 3' C‐O bond of the 2'‐H (DNA) nucleotides. Under beam‐type CID conditions, limited additional cleavage adjacent to 2'‐OMe substituted nucleotides was noted but 2'‐F modified residues remained resistant to cleavage. Copyright © 2012 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jms.2044
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Yang Gao<affiliation><mods:affiliation>Department of Chemistry, Purdue University, IN, 47907‐2084, West Lafayette</mods:affiliation><wicri:noCountry code="subField">Lafayette</wicri:noCountry></affiliation>Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Collision‐induced dissociation of oligonucleotide anions fully modified at the 2'‐position of the ribose: 2'‐F/‐H and 2'‐F/‐H/‐OMe mix‐mers</title><author><name sortKey="Gao, Yang" sort="Gao, Yang" uniqKey="Gao Y" first="Yang" last="Gao">Yang Gao</name><affiliation><mods:affiliation>Department of Chemistry, Purdue University, IN, 47907‐2084, West Lafayette</mods:affiliation><wicri:noCountry code="subField">Lafayette</wicri:noCountry></affiliation></author><author><name sortKey="Mcluckey, Scott A" sort="Mcluckey, Scott A" uniqKey="Mcluckey S" first="Scott A." last="Mcluckey">Scott A. Mcluckey</name><affiliation><mods:affiliation>Department of Chemistry, Purdue University, IN, 47907‐2084, West Lafayette</mods:affiliation><wicri:noCountry code="subField">Lafayette</wicri:noCountry></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: mcluckey@purdue.edu</mods:affiliation><country wicri:rule="url">États-Unis</country></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: mcluckey@purdue.edu</mods:affiliation><country wicri:rule="url">États-Unis</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:002CE167BA6543CF217792661FE63FA8EB7649F7</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1002/jms.2044</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-SWTT33C3-P/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000129</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000129</idno><idno type="wicri:Area/Istex/Curation">000129</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Collision‐induced dissociation of oligonucleotide anions fully modified at the 2'‐position of the ribose: 2'‐F/‐H and 2'‐F/‐H/‐OMe mix‐mers</title><author><name sortKey="Gao, Yang" sort="Gao, Yang" uniqKey="Gao Y" first="Yang" last="Gao">Yang Gao</name><affiliation><mods:affiliation>Department of Chemistry, Purdue University, IN, 47907‐2084, West Lafayette</mods:affiliation><wicri:noCountry code="subField">Lafayette</wicri:noCountry></affiliation></author><author><name sortKey="Mcluckey, Scott A" sort="Mcluckey, Scott A" uniqKey="Mcluckey S" first="Scott A." last="Mcluckey">Scott A. Mcluckey</name><affiliation><mods:affiliation>Department of Chemistry, Purdue University, IN, 47907‐2084, West Lafayette</mods:affiliation></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: mcluckey@purdue.edu</mods:affiliation><country wicri:rule="url">États-Unis</country></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: mcluckey@purdue.edu</mods:affiliation><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Mass Spectrometry</title><title level="j" type="alt">JOURNAL OF MASS SPECTROMETRY</title><idno type="ISSN">1076-5174</idno><idno type="eISSN">1096-9888</idno><imprint><biblScope unit="vol">47</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="364">364</biblScope><biblScope unit="page" to="369">369</biblScope><biblScope unit="page-count">6</biblScope><publisher>John Wiley & Sons, Ltd</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2012-03">2012-03</date></imprint><idno type="ISSN">1076-5174</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1076-5174</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Gas‐phase dissociation of various 2'‐position modified oligonucleotide anions has been studied as a function of precursor ion charge state using ion trap and low energy beam‐type collision‐induced dissociation (CID). For a completely 2'‐O‐methyl modified 6‐mer, all possible dissociation channels along the phosphodiester linkage, generating complementary (a‐B)/w‐, b/x‐, c/y‐, d/z‐ion series, were observed with no single dominant type of dissociation pathway. Full sequence information was generated from each charge state via ion trap CID. More sequential fragmentation was noted under beam‐type CID conditions. Comparison with model DNA, in which all 2'‐OH groups are converted to 2'‐H, and RNA anions suggests that the 2'‐OMe substitution stabilizes the phosphodiester linkage with respect to fragmentation relative to both DNA and RNA oligomers. For modified mix‐mer anions, comprised of DNA nucleotides and 2'‐F substituted nucleotides or a mixture of DNA nucleotides and 2'‐O‐methyl (2'‐OMe) and 2'‐F substituted nucleotides, 3'‐side backbone cleavage was found to be inhibited by the 2'‐OMe or 2'‐F modification on the nucleotides under ion trap CID conditions. Thus, the sequence information was limited to the a‐Base/w‐fragments from the cleavage of the 3' C‐O bond of the 2'‐H (DNA) nucleotides. Under beam‐type CID conditions, limited additional cleavage adjacent to 2'‐OMe substituted nucleotides was noted but 2'‐F modified residues remained resistant to cleavage. Copyright © 2012 John Wiley & Sons, Ltd.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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