Transcription in vivo within the replication origin of the Escherichia coli chromosome: a mechanism for activating initiation of replication
Identifieur interne : 000128 ( Istex/Curation ); précédent : 000127; suivant : 000129Transcription in vivo within the replication origin of the Escherichia coli chromosome: a mechanism for activating initiation of replication
Auteurs : Tsuneaki Asai [Japon] ; Chi-Pien Chen [Taïwan] ; Toshio Nagata [Japon] ; Mitsuru Takanami [Japon] ; Mutsuo Imai [Japon]Source :
- Molecular and General Genetics MGG [ 0026-8925 ] ; 1992-01-01.
English descriptors
- KwdEn :
- Teeft :
- Amber mutation, Asai, Bacterial strains, Bamhi, Biol, Chromosomal, Chromosome, Coli, Coli chromosome, Copy number, Deletion, Dnaa, Dnaa boxes, Dnaa gene, Dnaa protein, Dnaa strain, Duplex, Duplex opening, Escherichia, Escherichia coli, Escherichia coli chromosome, Fragment, Genet, Hindiii, Initiation event, Kornberg, Leftward, Leftward origin transcripts, Leftward transcription, Minimal oric, Mutation, Negative supercoiling, Nozaki, Nucleic acids, Oric, Oric fragment, Oric fragments, Oric plasmid replication, Oric plasmids, Oric region, Oric sequence, Origin activity, Phage, Plasmid, Polymerase, Positive supercoiling, Present work, Proc natl acad, Promoter, Promoter fragment, Replication, Replication origin, Right side, Rightward, Rightward origin transcription, Rightward origin transcripts, Rightward synthesis, Rightward transcription, Rightward transcripts, Rpob mutations, Sali, Schauzu, Sugimoto, Supercoiled, Supercoiling, Takanami, Transcript, Transcription, Transcription proceeds, Transcriptional, Transcriptional activation, Xhoi, Xhoi sites.
Abstract
Summary: Within the replication origin, oriC, of the Escherichia coli chromosome, novel in vivo transcripts were detected which proceeded rightward and whose production was activated by DnaA protein. In contrast, DnaA protein repressed the previously described ori-L leftward transcription. The former should introduce negative supercoiling, and the latter positive supercoiling, into the 13-mers. The effects of transcription on the initiation of replication were also investigated by making constructs with promoters placed near oriC. Transcription was found to enhance the origin activity only when it was oriented in such a way as to introduce negative supercoiling into the 13-mers. From these results, we propose that transcription within oriC regulates replication initiation by altering the topology of the 13-mer region.
Url:
DOI: 10.1007/BF00279788
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xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Microbiology, Kaohsiung Medical College, Kaohsiung</wicri:regionArea></affiliation></author><author><name sortKey="Nagata, Toshio" sort="Nagata, Toshio" uniqKey="Nagata T" first="Toshio" last="Nagata">Toshio Nagata</name><affiliation wicri:level="1"><mods:affiliation>Institute for Virus Research, Kyoto University, 606, Sakyo-ku, Kyoto, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Virus Research, Kyoto University, 606, Sakyo-ku, Kyoto</wicri:regionArea></affiliation></author><author><name sortKey="Takanami, Mitsuru" sort="Takanami, Mitsuru" uniqKey="Takanami M" first="Mitsuru" last="Takanami">Mitsuru Takanami</name><affiliation wicri:level="1"><mods:affiliation>Institute for Chemical Research, Kyoto University, 611, Uji, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Chemical Research, Kyoto University, 611, Uji</wicri:regionArea></affiliation></author><author><name sortKey="Imai, Mutsuo" sort="Imai, Mutsuo" uniqKey="Imai M" first="Mutsuo" last="Imai">Mutsuo Imai</name><affiliation wicri:level="1"><mods:affiliation>Institute for Virus Research, Kyoto University, 606, Sakyo-ku, Kyoto, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Virus Research, Kyoto University, 606, Sakyo-ku, Kyoto</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular and General Genetics MGG</title><title level="j" type="abbrev">Molec. Gen. Genet.</title><idno type="ISSN">0026-8925</idno><idno type="eISSN">1432-1874</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1992-01-01">1992-01-01</date><biblScope unit="volume">231</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="169">169</biblScope><biblScope unit="page" to="178">178</biblScope></imprint><idno type="ISSN">0026-8925</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0026-8925</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DnaA protein</term><term>Replication initiation</term><term>Transcription regulation</term><term>Transcriptional activation</term><term>oriC</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Amber mutation</term><term>Asai</term><term>Bacterial strains</term><term>Bamhi</term><term>Biol</term><term>Chromosomal</term><term>Chromosome</term><term>Coli</term><term>Coli chromosome</term><term>Copy number</term><term>Deletion</term><term>Dnaa</term><term>Dnaa boxes</term><term>Dnaa gene</term><term>Dnaa protein</term><term>Dnaa strain</term><term>Duplex</term><term>Duplex opening</term><term>Escherichia</term><term>Escherichia coli</term><term>Escherichia coli chromosome</term><term>Fragment</term><term>Genet</term><term>Hindiii</term><term>Initiation event</term><term>Kornberg</term><term>Leftward</term><term>Leftward origin transcripts</term><term>Leftward transcription</term><term>Minimal oric</term><term>Mutation</term><term>Negative supercoiling</term><term>Nozaki</term><term>Nucleic acids</term><term>Oric</term><term>Oric fragment</term><term>Oric fragments</term><term>Oric plasmid replication</term><term>Oric plasmids</term><term>Oric region</term><term>Oric sequence</term><term>Origin activity</term><term>Phage</term><term>Plasmid</term><term>Polymerase</term><term>Positive supercoiling</term><term>Present work</term><term>Proc natl acad</term><term>Promoter</term><term>Promoter fragment</term><term>Replication</term><term>Replication origin</term><term>Right side</term><term>Rightward</term><term>Rightward origin transcription</term><term>Rightward origin transcripts</term><term>Rightward synthesis</term><term>Rightward transcription</term><term>Rightward transcripts</term><term>Rpob mutations</term><term>Sali</term><term>Schauzu</term><term>Sugimoto</term><term>Supercoiled</term><term>Supercoiling</term><term>Takanami</term><term>Transcript</term><term>Transcription</term><term>Transcription proceeds</term><term>Transcriptional</term><term>Transcriptional activation</term><term>Xhoi</term><term>Xhoi sites</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Within the replication origin, oriC, of the Escherichia coli chromosome, novel in vivo transcripts were detected which proceeded rightward and whose production was activated by DnaA protein. In contrast, DnaA protein repressed the previously described ori-L leftward transcription. The former should introduce negative supercoiling, and the latter positive supercoiling, into the 13-mers. The effects of transcription on the initiation of replication were also investigated by making constructs with promoters placed near oriC. Transcription was found to enhance the origin activity only when it was oriented in such a way as to introduce negative supercoiling into the 13-mers. From these results, we propose that transcription within oriC regulates replication initiation by altering the topology of the 13-mer region.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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