Collision‐induced dissociation of oligonucleotide anions fully modified at the 2'‐position of the ribose: 2'‐F/‐H and 2'‐F/‐H/‐OMe mix‐mers
Identifieur interne : 000129 ( Istex/Corpus ); précédent : 000128; suivant : 000130Collision‐induced dissociation of oligonucleotide anions fully modified at the 2'‐position of the ribose: 2'‐F/‐H and 2'‐F/‐H/‐OMe mix‐mers
Auteurs : Yang Gao ; Scott A. McluckeySource :
- Journal of Mass Spectrometry [ 1076-5174 ] ; 2012-03.
Abstract
Gas‐phase dissociation of various 2'‐position modified oligonucleotide anions has been studied as a function of precursor ion charge state using ion trap and low energy beam‐type collision‐induced dissociation (CID). For a completely 2'‐O‐methyl modified 6‐mer, all possible dissociation channels along the phosphodiester linkage, generating complementary (a‐B)/w‐, b/x‐, c/y‐, d/z‐ion series, were observed with no single dominant type of dissociation pathway. Full sequence information was generated from each charge state via ion trap CID. More sequential fragmentation was noted under beam‐type CID conditions. Comparison with model DNA, in which all 2'‐OH groups are converted to 2'‐H, and RNA anions suggests that the 2'‐OMe substitution stabilizes the phosphodiester linkage with respect to fragmentation relative to both DNA and RNA oligomers. For modified mix‐mer anions, comprised of DNA nucleotides and 2'‐F substituted nucleotides or a mixture of DNA nucleotides and 2'‐O‐methyl (2'‐OMe) and 2'‐F substituted nucleotides, 3'‐side backbone cleavage was found to be inhibited by the 2'‐OMe or 2'‐F modification on the nucleotides under ion trap CID conditions. Thus, the sequence information was limited to the a‐Base/w‐fragments from the cleavage of the 3' C‐O bond of the 2'‐H (DNA) nucleotides. Under beam‐type CID conditions, limited additional cleavage adjacent to 2'‐OMe substituted nucleotides was noted but 2'‐F modified residues remained resistant to cleavage. Copyright © 2012 John Wiley & Sons, Ltd.
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DOI: 10.1002/jms.2044
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For a completely 2'‐O‐methyl modified 6‐mer, all possible dissociation channels along the phosphodiester linkage, generating complementary (a‐B)/w‐, b/x‐, c/y‐, d/z‐ion series, were observed with no single dominant type of dissociation pathway. Full sequence information was generated from each charge state via ion trap CID. More sequential fragmentation was noted under beam‐type CID conditions. Comparison with model DNA, in which all 2'‐OH groups are converted to 2'‐H, and RNA anions suggests that the 2'‐OMe substitution stabilizes the phosphodiester linkage with respect to fragmentation relative to both DNA and RNA oligomers. For modified mix‐mer anions, comprised of DNA nucleotides and 2'‐F substituted nucleotides or a mixture of DNA nucleotides and 2'‐O‐methyl (2'‐OMe) and 2'‐F substituted nucleotides, 3'‐side backbone cleavage was found to be inhibited by the 2'‐OMe or 2'‐F modification on the nucleotides under ion trap CID conditions. Thus, the sequence information was limited to the a‐Base/w‐fragments from the cleavage of the 3' C‐O bond of the 2'‐H (DNA) nucleotides. Under beam‐type CID conditions, limited additional cleavage adjacent to 2'‐OMe substituted nucleotides was noted but 2'‐F modified residues remained resistant to cleavage. 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For a completely 2'‐O‐methyl modified 6‐mer, all possible dissociation channels along the phosphodiester linkage, generating complementary (a‐B)/w‐, b/x‐, c/y‐, d/z‐ion series, were observed with no single dominant type of dissociation pathway. Full sequence information was generated from each charge state via ion trap CID. More sequential fragmentation was noted under beam‐type CID conditions. Comparison with model DNA, in which all 2'‐OH groups are converted to 2'‐H, and RNA anions suggests that the 2'‐OMe substitution stabilizes the phosphodiester linkage with respect to fragmentation relative to both DNA and RNA oligomers. For modified mix‐mer anions, comprised of DNA nucleotides and 2'‐F substituted nucleotides or a mixture of DNA nucleotides and 2'‐O‐methyl (2'‐OMe) and 2'‐F substituted nucleotides, 3'‐side backbone cleavage was found to be inhibited by the 2'‐OMe or 2'‐F modification on the nucleotides under ion trap CID conditions. Thus, the sequence information was limited to the a‐Base/w‐fragments from the cleavage of the 3' C‐O bond of the 2'‐H (DNA) nucleotides. Under beam‐type CID conditions, limited additional cleavage adjacent to 2'‐OMe substituted nucleotides was noted but 2'‐F modified residues remained resistant to cleavage. 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For modified mix‐mer anions, comprised of DNA nucleotides and 2'‐F substituted nucleotides or a mixture of DNA nucleotides and 2'‐O‐methyl (2'‐OMe) and 2'‐F substituted nucleotides, 3'‐side backbone cleavage was found to be inhibited by the 2'‐OMe or 2'‐F modification on the nucleotides under ion trap CID conditions. Thus, the sequence information was limited to the a‐Base/w‐fragments from the cleavage of the 3' C‐O bond of the 2'‐H (DNA) nucleotides. Under beam‐type CID conditions, limited additional cleavage adjacent to 2'‐OMe substituted nucleotides was noted but 2'‐F modified residues remained resistant to cleavage. 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Spectrom.</title></titleGroup></publicationMeta><publicationMeta level="part" position="30"><doi>10.1002/jms.v47.3</doi><copyright ownership="publisher">Copyright © 2012 John Wiley & Sons, Ltd.</copyright><numberingGroup><numbering type="journalVolume" number="47">47</numbering><numbering type="journalIssue">3</numbering></numberingGroup><coverDate startDate="2012-03">March 2012</coverDate></publicationMeta><publicationMeta level="unit" position="14" type="article" status="forIssue"><doi>10.1002/jms.2044</doi><idGroup><id type="unit" value="JMS2044"></id></idGroup><countGroup><count type="pageTotal" number="6"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Research Articles</title></titleGroup><copyright ownership="publisher">Copyright © 2012 John Wiley & Sons, Ltd.</copyright><eventGroup><event type="manuscriptReceived" date="2011-09-23"></event><event type="manuscriptRevised" date="2011-12-06"></event><event type="manuscriptAccepted" date="2011-12-09"></event><event type="xmlCreated" agent="SPi Global" date="2011-12-20"></event><event type="publishedOnlineFinalForm" date="2012-03-16"></event><event type="firstOnline" date="2012-03-16"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.3.4 mode:FullText" date="2015-02-24"></event></eventGroup><numberingGroup><numbering type="pageFirst">364</numbering><numbering type="pageLast">369</numbering></numberingGroup><correspondenceTo>Scott A. McLuckey, 560 Oval Drive, Department of Chemistry, Purdue University, West Lafayette, IN 47907–2084, USA. E‐mail: <email>mcluckey@purdue.edu</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JMS.JMS2044.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Collision‐induced dissociation of oligonucleotide anions fully modified at the 2'‐position of the ribose: 2'‐F/‐H and 2'‐F/‐H/‐OMe mix‐mers</title><title type="short">CID of 2'‐modified DNA mix‐mers</title><title type="shortAuthors">Y. Gao and S. A. McLuckey</title></titleGroup><creators><creator creatorRole="author" xml:id="jms2044-cr-0001" affiliationRef="#jms2044-aff-0001"><personName><givenNames>Yang</givenNames><familyName>Gao</familyName></personName></creator><creator creatorRole="author" xml:id="jms2044-cr-0002" affiliationRef="#jms2044-aff-0001" corresponding="yes"><personName><givenNames>Scott A.</givenNames><familyName>McLuckey</familyName></personName><contactDetails><email>mcluckey@purdue.edu</email><phone>(765) 494–5270</phone><fax>(765) 494–0239</fax></contactDetails></creator></creators><affiliationGroup><affiliation countryCode="US" type="organization" xml:id="jms2044-aff-0001"><orgDiv>Department of Chemistry</orgDiv><orgName>Purdue University</orgName><address><city>West Lafayette</city><countryPart>IN</countryPart><postCode>47907‐2084</postCode></address></affiliation></affiliationGroup><keywordGroup type="author"><keyword xml:id="jms2044-kwd-0001">ion trap CID</keyword><keyword xml:id="jms2044-kwd-0002">2'‐modified DNA</keyword><keyword xml:id="jms2044-kwd-0003">low energy beam‐type CID</keyword><keyword xml:id="jms2044-kwd-0004">DNA mixmers</keyword><keyword xml:id="jms2044-kwd-0005">nucleic acid tandem MS</keyword></keywordGroup><supportingInformation><p>Supporting information may be found in the online version of this article.</p><supportingInfoItem><mediaResource alt="supplementary data" mimeType="application/msword" href="urn-x:wiley:10765174:media:jms2044:jms_2044_sm_f1-3"></mediaResource><caption>Supporting Information</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main"><p>Gas‐phase dissociation of various 2'‐position modified oligonucleotide anions has been studied as a function of precursor ion charge state using ion trap and low energy beam‐type collision‐induced dissociation (CID). For a completely 2'‐O‐methyl modified 6‐mer, all possible dissociation channels along the phosphodiester linkage, generating complementary (a‐B)/w‐, b/x‐, c/y‐, d/z‐ion series, were observed with no single dominant type of dissociation pathway. Full sequence information was generated from each charge state via ion trap CID. More sequential fragmentation was noted under beam‐type CID conditions. Comparison with model DNA, in which all 2'‐OH groups are converted to 2'‐H, and RNA anions suggests that the 2'‐OMe substitution stabilizes the phosphodiester linkage with respect to fragmentation relative to both DNA and RNA oligomers. For modified mix‐mer anions, comprised of DNA nucleotides and 2'‐F substituted nucleotides or a mixture of DNA nucleotides and 2'‐O‐methyl (2'‐OMe) and 2'‐F substituted nucleotides, 3'‐side backbone cleavage was found to be inhibited by the 2'‐OMe or 2'‐F modification on the nucleotides under ion trap CID conditions. Thus, the sequence information was limited to the a‐Base/w‐fragments from the cleavage of the 3' C‐O bond of the 2'‐H (DNA) nucleotides. Under beam‐type CID conditions, limited additional cleavage adjacent to 2'‐OMe substituted nucleotides was noted but 2'‐F modified residues remained resistant to cleavage. Copyright © 2012 John Wiley & Sons, Ltd.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Collision‐induced dissociation of oligonucleotide anions fully modified at the 2'‐position of the ribose: 2'‐F/‐H and 2'‐F/‐H/‐OMe mix‐mers</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>CID of 2'‐modified DNA mix‐mers</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Collision‐induced dissociation of oligonucleotide anions fully modified at the 2'‐position of the ribose: 2'‐F/‐H and 2'‐F/‐H/‐OMe mix‐mers</title></titleInfo><name type="personal"><namePart type="given">Yang</namePart><namePart type="family">Gao</namePart><affiliation>Department of Chemistry, Purdue University, IN, 47907‐2084, West Lafayette</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Scott A.</namePart><namePart type="family">McLuckey</namePart><affiliation>Department of Chemistry, Purdue University, IN, 47907‐2084, West Lafayette</affiliation><affiliation>E-mail: mcluckey@purdue.edu</affiliation><affiliation>E-mail: mcluckey@purdue.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>John Wiley & Sons, Ltd</publisher><place><placeTerm type="text">Chichester, UK</placeTerm></place><dateIssued encoding="w3cdtf">2012-03</dateIssued><dateCreated encoding="w3cdtf">2011-12-20</dateCreated><dateCaptured encoding="w3cdtf">2011-09-23</dateCaptured><dateValid encoding="w3cdtf">2011-12-09</dateValid><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract>Gas‐phase dissociation of various 2'‐position modified oligonucleotide anions has been studied as a function of precursor ion charge state using ion trap and low energy beam‐type collision‐induced dissociation (CID). For a completely 2'‐O‐methyl modified 6‐mer, all possible dissociation channels along the phosphodiester linkage, generating complementary (a‐B)/w‐, b/x‐, c/y‐, d/z‐ion series, were observed with no single dominant type of dissociation pathway. Full sequence information was generated from each charge state via ion trap CID. More sequential fragmentation was noted under beam‐type CID conditions. Comparison with model DNA, in which all 2'‐OH groups are converted to 2'‐H, and RNA anions suggests that the 2'‐OMe substitution stabilizes the phosphodiester linkage with respect to fragmentation relative to both DNA and RNA oligomers. For modified mix‐mer anions, comprised of DNA nucleotides and 2'‐F substituted nucleotides or a mixture of DNA nucleotides and 2'‐O‐methyl (2'‐OMe) and 2'‐F substituted nucleotides, 3'‐side backbone cleavage was found to be inhibited by the 2'‐OMe or 2'‐F modification on the nucleotides under ion trap CID conditions. Thus, the sequence information was limited to the a‐Base/w‐fragments from the cleavage of the 3' C‐O bond of the 2'‐H (DNA) nucleotides. Under beam‐type CID conditions, limited additional cleavage adjacent to 2'‐OMe substituted nucleotides was noted but 2'‐F modified residues remained resistant to cleavage. Copyright © 2012 John Wiley & Sons, Ltd.</abstract><subject><genre>keywords</genre><topic>ion trap CID</topic><topic>2'‐modified DNA</topic><topic>low energy beam‐type CID</topic><topic>DNA mixmers</topic><topic>nucleic acid tandem MS</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Mass Spectrometry</title></titleInfo><titleInfo type="abbreviated"><title>J. Mass. Spectrom.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><note type="content"> Supporting information may be found in the online version of this article.Supporting Info Item: Supporting Information - </note><subject><genre>article-category</genre><topic>Research Article</topic><topic>Research Articles</topic></subject><identifier type="ISSN">1076-5174</identifier><identifier type="eISSN">1096-9888</identifier><identifier type="DOI">10.1002/(ISSN)1096-9888c</identifier><identifier type="PublisherID">JMS</identifier><part><date>2012</date><detail type="volume"><caption>vol.</caption><number>47</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>364</start><end>369</end><total>6</total></extent></part></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0001"><titleInfo><title>Potent and specific genetic interference by double‐stranded RNA in Caenorhabditis elegans</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Fire</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Xu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. K.</namePart><namePart type="family">Montgomery</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">Kostas</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. E.</namePart><namePart type="family">Driver</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. C.</namePart><namePart type="family">Mello</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">A. Fire, S. Xu, M. K. Montgomery, S. A. Kostas, S. E. Driver, C. C. Mello. Potent and specific genetic interference by double‐stranded RNA in Caenorhabditis elegans. Nature 1998, 391, 806–811.</note><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>391</number></detail><extent unit="pages"><start>806</start><end>811</end></extent></part><relatedItem type="host"><titleInfo><title>Nature</title></titleInfo><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>391</number></detail><extent unit="pages"><start>806</start><end>811</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0002"><titleInfo><title>RNAi therapeutics: Principles, prospects and challenges</title></titleInfo><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Aagaard</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. J.</namePart><namePart type="family">Rossi</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">L. Aagaard, J. J. Rossi. RNAi therapeutics: Principles, prospects and challenges. Adv. Drug Delivery Rev. 2007, 59, 75–86.</note><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>59</number></detail><extent unit="pages"><start>75</start><end>86</end></extent></part><relatedItem type="host"><titleInfo><title>Adv. Drug Delivery Rev.</title></titleInfo><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>59</number></detail><extent unit="pages"><start>75</start><end>86</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0003"><titleInfo><title>RNA targeting therapeutics: Molecular mechanisms of antisense oligonucleotides as a therapeutic platform</title></titleInfo><name type="personal"><namePart type="given">C. F.</namePart><namePart type="family">Bennett</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E. E.</namePart><namePart type="family">Swayze</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">C. F. Bennett, E. E. Swayze. RNA targeting therapeutics: Molecular mechanisms of antisense oligonucleotides as a therapeutic platform. Pharmacol. Toxicol. 2010, 50, 259–293.</note><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>50</number></detail><extent unit="pages"><start>259</start><end>293</end></extent></part><relatedItem type="host"><titleInfo><title>Pharmacol. Toxicol.</title></titleInfo><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>50</number></detail><extent unit="pages"><start>259</start><end>293</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0004"><titleInfo><title>RNA interference</title></titleInfo><name type="personal"><namePart type="given">G. J.</namePart><namePart type="family">Hannon</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">G. J. Hannon. RNA interference. Nature 2002, 418, 244–251.</note><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>418</number></detail><extent unit="pages"><start>244</start><end>251</end></extent></part><relatedItem type="host"><titleInfo><title>Nature</title></titleInfo><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>418</number></detail><extent unit="pages"><start>244</start><end>251</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0005"><titleInfo><title>Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Soutschek</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Akinc</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Bramlage</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Charisse</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Constien</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Donoghue</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Elbashir</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Geick</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Hadwiger</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Harborth</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">John</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Kesavan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Lavine</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. K.</namePart><namePart type="family">Pandey</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Racie</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. G.</namePart><namePart type="family">Rajeev</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Rohl</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Toudjarska</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Wang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Wuschko</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Bumcrot</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Koteliansky</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Limmer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Manoharan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Vornlocher</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">J. Soutschek, A. Akinc, B. Bramlage, K. Charisse, R. Constien, M. Donoghue, S. Elbashir, A. Geick, P. Hadwiger, J. Harborth, M. John, V. Kesavan, G. Lavine, R. K. Pandey, T. Racie, K. G. Rajeev, I. Rohl, I. Toudjarska, G. Wang, S. Wuschko, D. Bumcrot, V. Koteliansky, S. Limmer, M. Manoharan, H. Vornlocher. Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs. Nature 2004, 432, 173–178.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>432</number></detail><extent unit="pages"><start>173</start><end>178</end></extent></part><relatedItem type="host"><titleInfo><title>Nature</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>432</number></detail><extent unit="pages"><start>173</start><end>178</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0006"><titleInfo><title>Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi)</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Wilda</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">U.</namePart><namePart type="family">Fuchs</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Wossmann</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Borkhardt</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">M. Wilda, U. Fuchs, W. Wossmann, A. Borkhardt. Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi). Oncogene 2002, 21, 5716–5724.</note><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>21</number></detail><extent unit="pages"><start>5716</start><end>5724</end></extent></part><relatedItem type="host"><titleInfo><title>Oncogene</title></titleInfo><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>21</number></detail><extent unit="pages"><start>5716</start><end>5724</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0007"><titleInfo><title>Inhibition of hepatitis B virus in mice by RNA interference</title></titleInfo><name type="personal"><namePart type="given">A. P.</namePart><namePart type="family">McCaffery</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Nakai</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Pandey</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Z.</namePart><namePart type="family">Huang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F. H.</namePart><namePart type="family">Salazar</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Xu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. F.</namePart><namePart type="family">Wieland</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. L.</namePart><namePart type="family">Marion</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. A.</namePart><namePart type="family">Kay</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">A. P. McCaffery, H. Nakai, K. Pandey, Z. Huang, F. H. Salazar, H. Xu, S. F. Wieland, P. L. Marion, M. A. Kay. Inhibition of hepatitis B virus in mice by RNA interference. Nat. Biotechnol. 2003, 21, 639–644.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>21</number></detail><extent unit="pages"><start>639</start><end>644</end></extent></part><relatedItem type="host"><titleInfo><title>Nat. Biotechnol.</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>21</number></detail><extent unit="pages"><start>639</start><end>644</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0008"><titleInfo><title>Molecular medicine for the brain: silencing of disease genes with RNA interference</title></titleInfo><name type="personal"><namePart type="given">B. L.</namePart><namePart type="family">Davidson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H. L.</namePart><namePart type="family">Paulson</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">B. L. Davidson, H. L. Paulson. Molecular medicine for the brain: silencing of disease genes with RNA interference. Lancet Neurol. 2004, 3, 145–149.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>3</number></detail><extent unit="pages"><start>145</start><end>149</end></extent></part><relatedItem type="host"><titleInfo><title>Lancet Neurol.</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>3</number></detail><extent unit="pages"><start>145</start><end>149</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0009"><titleInfo><title>Characterization of fully 2'‐modified oligoribonucleotide hetero‐ and homoduplex hybridization and nuclease sensitivity</title></titleInfo><name type="personal"><namePart type="given">L. L.</namePart><namePart type="family">Cummins</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. R.</namePart><namePart type="family">Owens</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. M.</namePart><namePart type="family">Risen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E. A.</namePart><namePart type="family">Lesnik</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. M.</namePart><namePart type="family">Freier</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">McGee</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. J.</namePart><namePart type="family">Guinosso</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. D.</namePart><namePart type="family">Cook</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">L. L. Cummins, S. R. Owens, L. M. Risen, E. A. Lesnik, S. M. Freier, D. McGee, C. J. Guinosso, P. D. Cook. Characterization of fully 2'‐modified oligoribonucleotide hetero‐ and homoduplex hybridization and nuclease sensitivity. Nucleic Acid Res. 1995, 23, 2019–2024.</note><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>2019</start><end>2024</end></extent></part><relatedItem type="host"><titleInfo><title>Nucleic Acid Res.</title></titleInfo><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>2019</start><end>2024</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0010"><titleInfo><title>RNA interference in mammalian cells by chemically‐modified RNA</title></titleInfo><name type="personal"><namePart type="given">D. A.</namePart><namePart type="family">Braasch</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Jensen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Kaur</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Arar</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. A.</namePart><namePart type="family">White</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D. R.</namePart><namePart type="family">Corey</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">D. A. Braasch, S. Jensen, Y. Liu, K. Kaur, K. Arar, M. A. White, D. R. Corey. RNA interference in mammalian cells by chemically‐modified RNA. Biochemistry 2003, 42, 7967–7975.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>7967</start><end>7975</end></extent></part><relatedItem type="host"><titleInfo><title>Biochemistry</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>7967</start><end>7975</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0011"><titleInfo><title>Sequence‐specific potent induction of IFN‐alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7</title></titleInfo><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Hornung</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Guenthner‐Biller</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Bourquin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Ablasser</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Schlee</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Uematsu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Noronha</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Manoharan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Akira</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Fougerolles</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Endres</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Hartmann</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">V. Hornung, M. Guenthner‐Biller, C. Bourquin, A. Ablasser, M. Schlee, S. Uematsu, A. Noronha, M. Manoharan, S. Akira, A. Fougerolles, S. Endres, G. Hartmann. Sequence‐specific potent induction of IFN‐alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat. Med. 2005, 11, 263–270.</note><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>11</number></detail><extent unit="pages"><start>263</start><end>270</end></extent></part><relatedItem type="host"><titleInfo><title>Nat. Med.</title></titleInfo><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>11</number></detail><extent unit="pages"><start>263</start><end>270</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0012"><titleInfo><title>siRNA function in RNA: A chemical modification analysis</title></titleInfo><name type="personal"><namePart type="given">Y.‐L.</namePart><namePart type="family">Chiu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T. M.</namePart><namePart type="family">Rana</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Y.‐L. Chiu, T. M. Rana. siRNA function in RNA: A chemical modification analysis. RNA 2003, 9, 1034–1048.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>9</number></detail><extent unit="pages"><start>1034</start><end>1048</end></extent></part><relatedItem type="host"><titleInfo><title>RNA</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>9</number></detail><extent unit="pages"><start>1034</start><end>1048</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0013"><titleInfo><title>Potent and persistent in vivo anti‐HBV activity of chemically modified siRNAs</title></titleInfo><name type="personal"><namePart type="given">D. V.</namePart><namePart type="family">Morrissey</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. A.</namePart><namePart type="family">Lockridge</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Shaw</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Blanchard</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Jensen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Breen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Hartsough</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Machemer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Radka</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Jadhav</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N.</namePart><namePart type="family">Vaish</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Zinnen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Vargeese</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Bowman</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. S.</namePart><namePart type="family">Shaffer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. B.</namePart><namePart type="family">Jeffs</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Judge</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">MacLachlan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Polisky</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">D. V. Morrissey, J. A. Lockridge, L. Shaw, K. Blanchard, K. Jensen, W. Breen, K. Hartsough, L. Machemer, S. Radka, V. Jadhav, N. Vaish, S. Zinnen, C. Vargeese, K. Bowman, C. S. Shaffer, L. B. Jeffs, A. Judge, I. MacLachlan, B. Polisky. Potent and persistent in vivo anti‐HBV activity of chemically modified siRNAs. Nat. Biotechnol. 2005, 23, 1002–1007.</note><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>1002</start><end>1007</end></extent></part><relatedItem type="host"><titleInfo><title>Nat. Biotechnol.</title></titleInfo><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>1002</start><end>1007</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0014"><titleInfo><title>Experimental approaches to identify non‐coding RNAs</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Hüttenhofer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Vogel</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">A. Hüttenhofer, J. Vogel. Experimental approaches to identify non‐coding RNAs. Nucleic Acid Res. 2006, 34, 635–646.</note><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>34</number></detail><extent unit="pages"><start>635</start><end>646</end></extent></part><relatedItem type="host"><titleInfo><title>Nucleic Acid Res.</title></titleInfo><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>34</number></detail><extent unit="pages"><start>635</start><end>646</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0015"><titleInfo><title>Collision‐induced dissociation of intact duplex and single‐stranded siRNA Anions</title></titleInfo><name type="personal"><namePart type="given">T.‐Y.</namePart><namePart type="family">Huang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X.</namePart><namePart type="family">Liang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B. D. M.</namePart><namePart type="family">Hodges</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">T.‐Y. Huang, J. Liu, X. Liang, B. D. M. Hodges, S. A. McLuckey. Collision‐induced dissociation of intact duplex and single‐stranded siRNA Anions. Anal. Chem. 2008, 80, 8501–8508.</note><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>80</number></detail><extent unit="pages"><start>8501</start><end>8508</end></extent></part><relatedItem type="host"><titleInfo><title>Anal. Chem.</title></titleInfo><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>80</number></detail><extent unit="pages"><start>8501</start><end>8508</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0016"><titleInfo><title>Sequence confirmation of chemically modified RNAs using exonuclease digestion and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry</title></titleInfo><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Gao</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Rumley</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Yuan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Mao</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">H. Gao, Y. Liu, M. Rumley, H. Yuan, B. Mao. Sequence confirmation of chemically modified RNAs using exonuclease digestion and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. Rapid Commun. Mass Spectrom. 2009, 23, 3423–3430.</note><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>3423</start><end>3430</end></extent></part><relatedItem type="host"><titleInfo><title>Rapid Commun. Mass Spectrom.</title></titleInfo><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>3423</start><end>3430</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0017"><titleInfo><title>Tandem mass spectrometry of small, multiply charged oligonucleotides</title></titleInfo><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. J.</namePart><namePart type="family">Van Berkel</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. L.</namePart><namePart type="family">Glish</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">S. A. McLuckey, G. J. Van Berkel, G. L. Glish. Tandem mass spectrometry of small, multiply charged oligonucleotides. J. Am. Soc. Mass Spectrom. 1992, 3, 60–70.</note><part><date>1992</date><detail type="volume"><caption>vol.</caption><number>3</number></detail><extent unit="pages"><start>60</start><end>70</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>1992</date><detail type="volume"><caption>vol.</caption><number>3</number></detail><extent unit="pages"><start>60</start><end>70</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0018"><titleInfo><title>Tandem mass spectrometry of modified and platinated oligoribonucleotides</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Nyakas</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. R.</namePart><namePart type="family">Stucki</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Schürch</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">A. Nyakas, S. R. Stucki, S. Schürch. Tandem mass spectrometry of modified and platinated oligoribonucleotides. J. Am. Soc. Mass Spectrom. 2011, 22, 875–887.</note><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>22</number></detail><extent unit="pages"><start>875</start><end>887</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>22</number></detail><extent unit="pages"><start>875</start><end>887</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0019"><titleInfo><title>Implementation of ion/ion reactions in a quadrupole/time‐of‐flight tandem mass spectrometer</title></titleInfo><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Xia</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. A.</namePart><namePart type="family">Chrisman</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D. E.</namePart><namePart type="family">Erickson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X.</namePart><namePart type="family">Liang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F. A.</namePart><namePart type="family">Londry</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. J.</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Y. Xia, P. A. Chrisman, D. E. Erickson, J. Liu, X. Liang, F. A. Londry, M. J. Yang, S. A. McLuckey. Implementation of ion/ion reactions in a quadrupole/time‐of‐flight tandem mass spectrometer. Anal. Chem. 2006, 78, 4146–4154.</note><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>78</number></detail><extent unit="pages"><start>4146</start><end>4154</end></extent></part><relatedItem type="host"><titleInfo><title>Anal. Chem.</title></titleInfo><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>78</number></detail><extent unit="pages"><start>4146</start><end>4154</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0020"><titleInfo><title>Pulsed dual electrospray ionization for ion/ion reactions</title></titleInfo><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Xia</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X.</namePart><namePart type="family">Liang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Y. Xia, X. Liang, S. A. McLuckey. Pulsed dual electrospray ionization for ion/ion reactions. J. Am. Soc. Mass Spectom. 2005, 16, 1750–1756.</note><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>16</number></detail><extent unit="pages"><start>1750</start><end>1756</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectom.</title></titleInfo><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>16</number></detail><extent unit="pages"><start>1750</start><end>1756</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0021"><titleInfo><title>Evaluation of 2'‐modified oligonucleotides containing 2'deoxy gaps as antisense inhibitors of gene‐expression</title></titleInfo><name type="personal"><namePart type="given">B. P.</namePart><namePart type="family">Monia</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E. A.</namePart><namePart type="family">Lesnik</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Gonzalez</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W. F.</namePart><namePart type="family">Lima</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">McGee</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. J.</namePart><namePart type="family">Guinosso</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. M.</namePart><namePart type="family">Kawasaki</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. D.</namePart><namePart type="family">Cook</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. M.</namePart><namePart type="family">Freier</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">B. P. Monia, E. A. Lesnik, C. Gonzalez, W. F. Lima, D. McGee, C. J. Guinosso, A. M. Kawasaki, P. D. Cook, S. M. Freier. Evaluation of 2'‐modified oligonucleotides containing 2'deoxy gaps as antisense inhibitors of gene‐expression. J. Bio. Chem. 1993, 268, 14514–14522.</note><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>268</number></detail><extent unit="pages"><start>14514</start><end>14522</end></extent></part><relatedItem type="host"><titleInfo><title>J. Bio. Chem.</title></titleInfo><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>268</number></detail><extent unit="pages"><start>14514</start><end>14522</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0022"><titleInfo><title>Ultra‐performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) and UPLC/MSE analysis of RNA oligonucleotides</title></titleInfo><name type="personal"><namePart type="given">V. B.</namePart><namePart type="family">Ivleva</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y. Q.</namePart><namePart type="family">Yu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Gilar</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">V. B. Ivleva, Y. Q. Yu, M. Gilar. Ultra‐performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) and UPLC/MSE analysis of RNA oligonucleotides. Rapid Commun. Mass Spectrom. 2010, 24, 2631–2640.</note><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>24</number></detail><extent unit="pages"><start>2631</start><end>2640</end></extent></part><relatedItem type="host"><titleInfo><title>Rapid Commun. Mass Spectrom.</title></titleInfo><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>24</number></detail><extent unit="pages"><start>2631</start><end>2640</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0023"><titleInfo><title>Charged vs. neutral nucleobase loss from multiply charged oligonucleotide anions</title></titleInfo><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Vaidyanathan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Habibi‐Goudarzi</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">S. A. McLuckey, G. Vaidyanathan, S. Habibi‐Goudarzi. Charged vs. neutral nucleobase loss from multiply charged oligonucleotide anions. J. Mass Spectrom. 1995, 30, 1222–1229.</note><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>30</number></detail><extent unit="pages"><start>1222</start><end>1229</end></extent></part><relatedItem type="host"><titleInfo><title>J. Mass Spectrom.</title></titleInfo><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>30</number></detail><extent unit="pages"><start>1222</start><end>1229</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0024"><titleInfo><title>Investigation of the initial fragmentation of oligodeoxynucleotides in a quadrupole ion trap: Charge level‐related base loss</title></titleInfo><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Pan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Verhoeven</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. K.</namePart><namePart type="family">Lee</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">S. Pan, K. Verhoeven, J. K. Lee. Investigation of the initial fragmentation of oligodeoxynucleotides in a quadrupole ion trap: Charge level‐related base loss. J. Am. Soc. Mass Spectrom. 2005, 16, 1853–1865.</note><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>16</number></detail><extent unit="pages"><start>1853</start><end>1865</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>16</number></detail><extent unit="pages"><start>1853</start><end>1865</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0025"><titleInfo><title>Gas‐phase fragmentation of oligonucleotide ions</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Wu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">J. Wu, S. A. McLuckey. Gas‐phase fragmentation of oligonucleotide ions. Int. J. Mass Spectrom. 2004, 237, 197–241.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>237</number></detail><extent unit="pages"><start>197</start><end>241</end></extent></part><relatedItem type="host"><titleInfo><title>Int. J. Mass Spectrom.</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>237</number></detail><extent unit="pages"><start>197</start><end>241</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0026"><titleInfo><title>Ion trap collision‐induced dissociation of multiply deprotonated RNA: c/y‐ions versus (a‐B)/w‐ions</title></titleInfo><name type="personal"><namePart type="given">T.‐Y.</namePart><namePart type="family">Huang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Karlamova</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">T.‐Y. Huang, A. Karlamova, J. Liu, S. A. McLuckey. Ion trap collision‐induced dissociation of multiply deprotonated RNA: c/y‐ions versus (a‐B)/w‐ions. J. Am. Soc. Mass Spectrom. 2008, 19, 1832–1840.</note><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>19</number></detail><extent unit="pages"><start>1832</start><end>1840</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>19</number></detail><extent unit="pages"><start>1832</start><end>1840</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0027"><titleInfo><title>New Aspects of the Fragmentation Mechanisms of Unmodified and Methylphosphonate‐Modified Oligonucleotides</title></titleInfo><name type="personal"><namePart type="given">S. T. M.</namePart><namePart type="family">Monn</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Schürch</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">S. T. M. Monn, S. Schürch. New Aspects of the Fragmentation Mechanisms of Unmodified and Methylphosphonate‐Modified Oligonucleotides. J. Am. Soc. Mass Spectrom. 2007, 18, 984–990.</note><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>18</number></detail><extent unit="pages"><start>984</start><end>990</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>18</number></detail><extent unit="pages"><start>984</start><end>990</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0028"><titleInfo><title>Low‐energy collision‐induced dissociation of deprotonated dinucleotides: Determination of the energetically favored dissociation pathways and the relative acidities of the nucleic acid bases</title></titleInfo><name type="personal"><namePart type="given">M. T.</namePart><namePart type="family">Rodgers</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Campbell</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E. M.</namePart><namePart type="family">Marzluff</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. L.</namePart><namePart type="family">Beauchamp</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">M. T. Rodgers, S. Campbell, E. M. Marzluff, J. L. Beauchamp. Low‐energy collision‐induced dissociation of deprotonated dinucleotides: Determination of the energetically favored dissociation pathways and the relative acidities of the nucleic acid bases. Int. J. Mass Spectrom. 1994, 137, 121–149.</note><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>137</number></detail><extent unit="pages"><start>121</start><end>149</end></extent></part><relatedItem type="host"><titleInfo><title>Int. J. Mass Spectrom.</title></titleInfo><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>137</number></detail><extent unit="pages"><start>121</start><end>149</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0029"><titleInfo><title>Ion trap collisional activation of the deprotonated deoxymononucleoside and deoxydinucleoside monophosphates</title></titleInfo><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Habibi‐Ghoudarzi</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">S. Habibi‐Ghoudarzi, S. A. McLuckey. Ion trap collisional activation of the deprotonated deoxymononucleoside and deoxydinucleoside monophosphates. J. Am. Soc. Mass Spectrom. 1995, 6, 102–113.</note><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>6</number></detail><extent unit="pages"><start>102</start><end>113</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>6</number></detail><extent unit="pages"><start>102</start><end>113</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0030"><titleInfo><title>Gas‐Phase dissociation of oligonucleotides and their analogues studied by electrospray ionization tandem mass spectrometry</title></titleInfo><name type="personal"><namePart type="given">J. M.</namePart><namePart type="family">Tromp</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Schürch</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">J. M. Tromp, S. Schürch. Gas‐Phase dissociation of oligonucleotides and their analogues studied by electrospray ionization tandem mass spectrometry. J. Am. Soc. Mass Spectrom. 2005, 16, 1262–1268.</note><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>16</number></detail><extent unit="pages"><start>1262</start><end>1268</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>16</number></detail><extent unit="pages"><start>1262</start><end>1268</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0031"><titleInfo><title>Electrospray tandem mass spectrometry of mixed‐sequence RNA/DNA oligonucleotides</title></titleInfo><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Schürch</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Bernal‐Mendez</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. J.</namePart><namePart type="family">Leumann</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">S. Schürch, E. Bernal‐Mendez, C. J. Leumann. Electrospray tandem mass spectrometry of mixed‐sequence RNA/DNA oligonucleotides. J. Am. Soc. Mass Spectrom. 2002, 13, 936–945.</note><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>936</start><end>945</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>936</start><end>945</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0032"><titleInfo><title>RNA Fragmentation in MALDI mass spectrometry studied by H/D‐exchange: Mechanism of general applicability to nucleic acids</title></titleInfo><name type="personal"><namePart type="given">T. E.</namePart><namePart type="family">Andersen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Kirpekar</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. F.</namePart><namePart type="family">Haselmann</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">T. E. Andersen, F. Kirpekar, K. F. Haselmann. RNA Fragmentation in MALDI mass spectrometry studied by H/D‐exchange: Mechanism of general applicability to nucleic acids. J. Am. Soc. Mass Spectrom. 2006, 17, 1353–1368.</note><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>17</number></detail><extent unit="pages"><start>1353</start><end>1368</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>17</number></detail><extent unit="pages"><start>1353</start><end>1368</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0033"><titleInfo><title>Collision‐induced dissociation (CID) of peptides and proteins</title></titleInfo><name type="personal"><namePart type="given">J. M.</namePart><namePart type="family">Wells</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">J. M. Wells, S. A. McLuckey. Collision‐induced dissociation (CID) of peptides and proteins. Methods Enzymol. 2005, 402, 148–185.</note><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>402</number></detail><extent unit="pages"><start>148</start><end>185</end></extent></part><relatedItem type="host"><titleInfo><title>Methods Enzymol.</title></titleInfo><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>402</number></detail><extent unit="pages"><start>148</start><end>185</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0034"><titleInfo><title>Sequence confirmation of modified oligonucleotides using chemical degradation, electrospray ionization, time‐of‐flight, and tandem mass spectrometry</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Farand</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Beverly</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">J. Farand, M. Beverly. Sequence confirmation of modified oligonucleotides using chemical degradation, electrospray ionization, time‐of‐flight, and tandem mass spectrometry. Anal. Chem. 2008, 80, 7414–7421.</note><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>80</number></detail><extent unit="pages"><start>7414</start><end>7421</end></extent></part><relatedItem type="host"><titleInfo><title>Anal. Chem.</title></titleInfo><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>80</number></detail><extent unit="pages"><start>7414</start><end>7421</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0035"><titleInfo><title>Ion trap collision‐induced dissociation of locked nucleic acids</title></titleInfo><name type="personal"><namePart type="given">T.‐Y.</namePart><namePart type="family">Huang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Kharlamova</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. A.</namePart><namePart type="family">McLuckey</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">T.‐Y. Huang, A. Kharlamova, S. A. McLuckey. Ion trap collision‐induced dissociation of locked nucleic acids. J. Am. Soc. Mass Spectrom. 2010, 21, 144–153.</note><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>21</number></detail><extent unit="pages"><start>144</start><end>153</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>21</number></detail><extent unit="pages"><start>144</start><end>153</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="jms2044-cit-0036"><titleInfo><title>Structure and fragmentation mechanisms of isomeric T‐rich oligodeoxynucleotides: A comparison of four tandem mass spectrometric methods</title></titleInfo><name type="personal"><namePart type="given">Z.</namePart><namePart type="family">Wang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. X.</namePart><namePart type="family">Wan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Ramanathan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. S.</namePart><namePart type="family">Taylor</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. L</namePart><namePart type="family">Gross</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Z. Wang, K. X. Wan, R. Ramanathan, J. S. Taylor, M. L Gross. Structure and fragmentation mechanisms of isomeric T‐rich oligodeoxynucleotides: A comparison of four tandem mass spectrometric methods. J. Am. Soc. Mass Spectrom.1998, 9, 683–691.</note><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>9</number></detail><extent unit="pages"><start>683</start><end>691</end></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Soc. Mass Spectrom.</title></titleInfo><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>9</number></detail><extent unit="pages"><start>683</start><end>691</end></extent></part></relatedItem></relatedItem><identifier type="istex">002CE167BA6543CF217792661FE63FA8EB7649F7</identifier><identifier type="ark">ark:/67375/WNG-SWTT33C3-P</identifier><identifier type="DOI">10.1002/jms.2044</identifier><identifier type="ArticleID">JMS2044</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2012 John Wiley & Sons, Ltd.Copyright © 2012 John Wiley & Sons, Ltd.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-15</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-SWTT33C3-P/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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