Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses
Identifieur interne : 000380 ( France/Extraction ); précédent : 000379; suivant : 000381Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses
Auteurs : Pascal Genschik [France] ; Yves Parmentier [France] ; Andrée Durr [France] ; Jacqueline Marbach [France] ; Marie-Claire Criqui [France] ; Elisabeth Jamet [France] ; Jacqueline Fleck [France]Source :
- Plant Molecular Biology [ 0167-4412 ] ; 1992-12-01.
Descripteurs français
- KwdFr :
- ADN (génétique), ADN (isolement et purification), ARN (génétique), ARN (isolement et purification), ARN messager (biosynthèse), ARN messager (isolement et purification), Cartographie de restriction, Cellules cultivées, Données de séquences moléculaires, Famille multigénique, Humains, Masse moléculaire, Phénomènes physiologiques des plantes, Plantes (génétique), Protoplastes (physiologie), Régulation de l'expression des gènes, Similitude de séquences d'acides aminés, Similitude de séquences d'acides nucléiques, Sondes d'ADN, Séquence d'acides aminés, Séquence nucléotidique, Tabac (génétique), Tabac (physiologie), Ubiquitines (génétique), Végétaux toxiques.
- MESH :
- biosynthèse : ARN messager.
- génétique : ADN, ARN, Plantes, Tabac, Ubiquitines.
- isolement et purification : ADN, ARN, ARN messager.
- physiologie : Protoplastes, Tabac.
- Cartographie de restriction, Cellules cultivées, Données de séquences moléculaires, Famille multigénique, Humains, Masse moléculaire, Phénomènes physiologiques des plantes, Régulation de l'expression des gènes, Similitude de séquences d'acides aminés, Similitude de séquences d'acides nucléiques, Sondes d'ADN, Séquence d'acides aminés, Séquence nucléotidique, Végétaux toxiques.
English descriptors
- KwdEn :
- Amino Acid Sequence, Base Sequence, Cell division, Cells, Cultured, DNA (genetics), DNA (isolation & purification), DNA Probes, Gene Expression Regulation, Humans, Molecular Sequence Data, Molecular Weight, Multigene Family, Nicotiana sylvestris, Plant Physiological Phenomena, Plants (genetics), Plants, Toxic, Protoplasts (physiology), RNA (genetics), RNA (isolation & purification), RNA, Messenger (biosynthesis), RNA, Messenger (isolation & purification), Restriction Mapping, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Tobacco (genetics), Tobacco (physiology), Ubiquitins (genetics), gene expression, protoplast, stress, ubiquitin.
- MESH :
- chemical , biosynthesis : RNA, Messenger.
- chemical , genetics : DNA, RNA, Ubiquitins.
- chemical , isolation & purification : DNA, RNA, RNA, Messenger.
- genetics : Plants, Tobacco.
- physiology : Protoplasts, Tobacco.
- Teeft :
- Amino, Amino Acid Sequence, Amino acid, Amino acid sequence, Amino acid sequences, Amino acids, Arabidopsis thaliana, Basal level, Base Sequence, Biochem, Biol, Blot analysis, Carboxyl extension protein, Cdna, Cell biol, Cell cycle, Cell division, Cell suspension cultures, Cells, Cultured, Clone, Corresponding genes, DNA Probes, Durr, Encode, Encoding, Encoding genes, First divisions, Fleck, Gene, Gene Expression Regulation, Gene expression, Heat shock, High level, Human probe, Human region, Human ubiquitin, Humans, Hybridization, Hypersensitive reaction, Isolation procedure, Leaf strips, Mercuric, Mercuric chloride solution, Mercuric chloride treatment, Molecular Sequence Data, Molecular Weight, Mrna, Mrnas encode, Multigene Family, Nicotiana, Nicotiana sylvestris, Nucl acids, Nucleotide, Nylon membrane, Other hand, Plant Physiological Phenomena, Plants, Toxic, Polyubiquitin, Polyubiquitin genes, Polyubiquitin mrnas, Probe, Proc natl acad, Protein synthesis, Protoplast, Protoplast culture, Restriction Mapping, Rna, Same membrane, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Short time, Size class, Size classes, Specific probes, Sterile water, Sylvestris, Sylvestris plants, Thaliana, Total rnas, Ubiquitin, Ubiquitin gene expression, Ubiquitin genes, Ubiquitin monomer, Ubiquitin monomers, Ubiquitin mrnas, Ubiquitin multigene family, Ubiquitin transcript levels, Ubiquitin transcripts, Various stresses, Various times, Viral infection, Yeast.
Abstract
Abstract: Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5- mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.
Url:
DOI: 10.1007/BF00027161
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<title level="j" type="sub">An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</title>
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<term>Base Sequence</term>
<term>Cell division</term>
<term>Cells, Cultured</term>
<term>DNA (genetics)</term>
<term>DNA (isolation & purification)</term>
<term>DNA Probes</term>
<term>Gene Expression Regulation</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Molecular Weight</term>
<term>Multigene Family</term>
<term>Nicotiana sylvestris</term>
<term>Plant Physiological Phenomena</term>
<term>Plants (genetics)</term>
<term>Plants, Toxic</term>
<term>Protoplasts (physiology)</term>
<term>RNA (genetics)</term>
<term>RNA (isolation & purification)</term>
<term>RNA, Messenger (biosynthesis)</term>
<term>RNA, Messenger (isolation & purification)</term>
<term>Restriction Mapping</term>
<term>Sequence Homology, Amino Acid</term>
<term>Sequence Homology, Nucleic Acid</term>
<term>Tobacco (genetics)</term>
<term>Tobacco (physiology)</term>
<term>Ubiquitins (genetics)</term>
<term>gene expression</term>
<term>protoplast</term>
<term>stress</term>
<term>ubiquitin</term>
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<term>ARN messager (isolement et purification)</term>
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<term>Phénomènes physiologiques des plantes</term>
<term>Plantes (génétique)</term>
<term>Protoplastes (physiologie)</term>
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<term>ARN</term>
<term>Plantes</term>
<term>Tabac</term>
<term>Ubiquitines</term>
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<term>ARN</term>
<term>ARN messager</term>
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<term>Amino Acid Sequence</term>
<term>Amino acid</term>
<term>Amino acid sequence</term>
<term>Amino acid sequences</term>
<term>Amino acids</term>
<term>Arabidopsis thaliana</term>
<term>Basal level</term>
<term>Base Sequence</term>
<term>Biochem</term>
<term>Biol</term>
<term>Blot analysis</term>
<term>Carboxyl extension protein</term>
<term>Cdna</term>
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<term>Cell cycle</term>
<term>Cell division</term>
<term>Cell suspension cultures</term>
<term>Cells, Cultured</term>
<term>Clone</term>
<term>Corresponding genes</term>
<term>DNA Probes</term>
<term>Durr</term>
<term>Encode</term>
<term>Encoding</term>
<term>Encoding genes</term>
<term>First divisions</term>
<term>Fleck</term>
<term>Gene</term>
<term>Gene Expression Regulation</term>
<term>Gene expression</term>
<term>Heat shock</term>
<term>High level</term>
<term>Human probe</term>
<term>Human region</term>
<term>Human ubiquitin</term>
<term>Humans</term>
<term>Hybridization</term>
<term>Hypersensitive reaction</term>
<term>Isolation procedure</term>
<term>Leaf strips</term>
<term>Mercuric</term>
<term>Mercuric chloride solution</term>
<term>Mercuric chloride treatment</term>
<term>Molecular Sequence Data</term>
<term>Molecular Weight</term>
<term>Mrna</term>
<term>Mrnas encode</term>
<term>Multigene Family</term>
<term>Nicotiana</term>
<term>Nicotiana sylvestris</term>
<term>Nucl acids</term>
<term>Nucleotide</term>
<term>Nylon membrane</term>
<term>Other hand</term>
<term>Plant Physiological Phenomena</term>
<term>Plants, Toxic</term>
<term>Polyubiquitin</term>
<term>Polyubiquitin genes</term>
<term>Polyubiquitin mrnas</term>
<term>Probe</term>
<term>Proc natl acad</term>
<term>Protein synthesis</term>
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<term>Protoplast culture</term>
<term>Restriction Mapping</term>
<term>Rna</term>
<term>Same membrane</term>
<term>Sequence Homology, Amino Acid</term>
<term>Sequence Homology, Nucleic Acid</term>
<term>Short time</term>
<term>Size class</term>
<term>Size classes</term>
<term>Specific probes</term>
<term>Sterile water</term>
<term>Sylvestris</term>
<term>Sylvestris plants</term>
<term>Thaliana</term>
<term>Total rnas</term>
<term>Ubiquitin</term>
<term>Ubiquitin gene expression</term>
<term>Ubiquitin genes</term>
<term>Ubiquitin monomer</term>
<term>Ubiquitin monomers</term>
<term>Ubiquitin mrnas</term>
<term>Ubiquitin multigene family</term>
<term>Ubiquitin transcript levels</term>
<term>Ubiquitin transcripts</term>
<term>Various stresses</term>
<term>Various times</term>
<term>Viral infection</term>
<term>Yeast</term>
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<term>Cellules cultivées</term>
<term>Données de séquences moléculaires</term>
<term>Famille multigénique</term>
<term>Humains</term>
<term>Masse moléculaire</term>
<term>Phénomènes physiologiques des plantes</term>
<term>Régulation de l'expression des gènes</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>Sondes d'ADN</term>
<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">Abstract: Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5- mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.</div>
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<tree><country name="France"><region name="Grand Est"><name sortKey="Genschik, Pascal" sort="Genschik, Pascal" uniqKey="Genschik P" first="Pascal" last="Genschik">Pascal Genschik</name>
</region>
<name sortKey="Criqui, Marie Claire" sort="Criqui, Marie Claire" uniqKey="Criqui M" first="Marie-Claire" last="Criqui">Marie-Claire Criqui</name>
<name sortKey="Durr, Andree" sort="Durr, Andree" uniqKey="Durr A" first="Andrée" last="Durr">Andrée Durr</name>
<name sortKey="Fleck, Jacqueline" sort="Fleck, Jacqueline" uniqKey="Fleck J" first="Jacqueline" last="Fleck">Jacqueline Fleck</name>
<name sortKey="Jamet, Elisabeth" sort="Jamet, Elisabeth" uniqKey="Jamet E" first="Elisabeth" last="Jamet">Elisabeth Jamet</name>
<name sortKey="Marbach, Jacqueline" sort="Marbach, Jacqueline" uniqKey="Marbach J" first="Jacqueline" last="Marbach">Jacqueline Marbach</name>
<name sortKey="Parmentier, Yves" sort="Parmentier, Yves" uniqKey="Parmentier Y" first="Yves" last="Parmentier">Yves Parmentier</name>
</country>
</tree>
</affiliations>
</record>
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