Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses
Identifieur interne : 000590 ( Istex/Corpus ); précédent : 000589; suivant : 000591Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses
Auteurs : Pascal Genschik ; Yves Parmentier ; Andrée Durr ; Jacqueline Marbach ; Marie-Claire Criqui ; Elisabeth Jamet ; Jacqueline FleckSource :
- Plant Molecular Biology [ 0167-4412 ] ; 1992-12-01.
English descriptors
- KwdEn :
- Teeft :
- Amino, Amino acid, Amino acid sequence, Amino acid sequences, Amino acids, Arabidopsis thaliana, Basal level, Biochem, Biol, Blot analysis, Carboxyl extension protein, Cdna, Cell biol, Cell cycle, Cell division, Cell suspension cultures, Clone, Corresponding genes, Durr, Encode, Encoding, Encoding genes, First divisions, Fleck, Gene, Gene expression, Heat shock, High level, Human probe, Human region, Human ubiquitin, Hybridization, Hypersensitive reaction, Isolation procedure, Leaf strips, Mercuric, Mercuric chloride solution, Mercuric chloride treatment, Mrna, Mrnas encode, Nicotiana, Nicotiana sylvestris, Nucl acids, Nucleotide, Nylon membrane, Other hand, Polyubiquitin, Polyubiquitin genes, Polyubiquitin mrnas, Probe, Proc natl acad, Protein synthesis, Protoplast, Protoplast culture, Rna, Same membrane, Short time, Size class, Size classes, Specific probes, Sterile water, Sylvestris, Sylvestris plants, Thaliana, Total rnas, Ubiquitin, Ubiquitin gene expression, Ubiquitin genes, Ubiquitin monomer, Ubiquitin monomers, Ubiquitin mrnas, Ubiquitin multigene family, Ubiquitin transcript levels, Ubiquitin transcripts, Various stresses, Various times, Viral infection, Yeast.
Abstract
Abstract: Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5- mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.
Url:
DOI: 10.1007/BF00027161
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ISTEX:33D1C251C3F6EC1B5503A2CEE68D2470C907AB9FLe document en format XML
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of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5- mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.</div></front></TEI><istex><corpusName>springer-journals</corpusName><keywords><teeft><json:string>ubiquitin</json:string><json:string>protoplast</json:string><json:string>mrna</json:string><json:string>sylvestris</json:string><json:string>polyubiquitin</json:string><json:string>cdna</json:string><json:string>clone</json:string><json:string>encoding</json:string><json:string>ubiquitin genes</json:string><json:string>heat shock</json:string><json:string>nicotiana</json:string><json:string>biol</json:string><json:string>specific probes</json:string><json:string>rna</json:string><json:string>hybridization</json:string><json:string>thaliana</json:string><json:string>amino</json:string><json:string>size classes</json:string><json:string>encode</json:string><json:string>nicotiana 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Durr</name><affiliations><json:string>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</json:string></affiliations></json:item><json:item><name>Jacqueline Marbach</name><affiliations><json:string>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</json:string></affiliations></json:item><json:item><name>Marie-Claire Criqui</name><affiliations><json:string>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</json:string></affiliations></json:item><json:item><name>Elisabeth Jamet</name><affiliations><json:string>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</json:string></affiliations></json:item><json:item><name>Jacqueline Fleck</name><affiliations><json:string>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Cell division</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>gene expression</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Nicotiana sylvestris</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>protoplast</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>stress</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ubiquitin</value></json:item></subject><articleId><json:string>BF00027161</json:string><json:string>Art15</json:string></articleId><arkIstex>ark:/67375/1BB-K0DM107N-M</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5- mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.</abstract><qualityIndicators><score>9.7</score><pdfWordCount>8144</pdfWordCount><pdfCharCount>43758</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>14</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>225</abstractWordCount><abstractCharCount>1422</abstractCharCount><keywordCount>6</keywordCount></qualityIndicators><title>Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses</title><pmid><json:string>1281439</json:string></pmid><genre><json:string>research-article</json:string></genre><host><title>Plant Molecular 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of Nicotiana sylvestris and in response to various stresses</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><availability><licence><p>Kluwer Academic Publishers, 1992</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>1992-04-07</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>Research Articles</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses</title><author xml:id="author-0000"><persName><forename type="first">Pascal</forename><surname>Genschik</surname></persName><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Yves</forename><surname>Parmentier</surname></persName><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Andrée</forename><surname>Durr</surname></persName><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Jacqueline</forename><surname>Marbach</surname></persName><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Marie-Claire</forename><surname>Criqui</surname></persName><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Elisabeth</forename><surname>Jamet</surname></persName><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation></author><author xml:id="author-0006"><persName><forename type="first">Jacqueline</forename><surname>Fleck</surname></persName><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation></author><idno type="istex">33D1C251C3F6EC1B5503A2CEE68D2470C907AB9F</idno><idno type="ark">ark:/67375/1BB-K0DM107N-M</idno><idno type="DOI">10.1007/BF00027161</idno><idno type="article-id">BF00027161</idno><idno type="article-id">Art15</idno></analytic><monogr><title level="j">Plant Molecular Biology</title><title level="j" type="sub">An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</title><title level="j" type="abbrev">Plant Mol Biol</title><idno type="pISSN">0167-4412</idno><idno type="eISSN">1573-5028</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">24</idno><idno type="volume-issue-count">6</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1992-12-01"></date><biblScope unit="volume">20</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="897">897</biblScope><biblScope unit="page" to="910">910</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1992-04-07</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5- mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Key words</head><item><term>Cell division</term></item><item><term>gene expression</term></item><item><term>Nicotiana sylvestris</term></item><item><term>protoplast</term></item><item><term>stress</term></item><item><term>ubiquitin</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Biochemistry, general</term></item><item><term>Plant Sciences</term></item><item><term>Plant Pathology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1992-04-07">Created</change><change when="1992-12-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-K0DM107N-M/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Kluwer Academic Publishers</PublisherName><PublisherLocation>Dordrecht</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>11103</JournalID><JournalPrintISSN>0167-4412</JournalPrintISSN><JournalElectronicISSN>1573-5028</JournalElectronicISSN><JournalTitle>Plant Molecular Biology</JournalTitle><JournalSubTitle>An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</JournalSubTitle><JournalAbbreviatedTitle>Plant Mol Biol</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Biochemistry, general</JournalSubject><JournalSubject Type="Secondary">Plant Sciences</JournalSubject><JournalSubject Type="Secondary">Plant Pathology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>20</VolumeIDStart><VolumeIDEnd>20</VolumeIDEnd><VolumeIssueCount>6</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>5</IssueIDStart><IssueIDEnd>5</IssueIDEnd><IssueArticleCount>24</IssueArticleCount><IssueHistory><CoverDate><Year>1992</Year><Month>12</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName><CopyrightYear>1992</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art15"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF00027161</ArticleID><ArticleDOI>10.1007/BF00027161</ArticleDOI><ArticleSequenceNumber>15</ArticleSequenceNumber><ArticleTitle Language="En">Ubiquitin genes are differentially regulated in protoplast-derived cultures of <Emphasis Type="Italic">Nicotiana sylvestris</Emphasis> and in response to various stresses</ArticleTitle><ArticleCategory>Research Articles</ArticleCategory><ArticleFirstPage>897</ArticleFirstPage><ArticleLastPage>910</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>4</Month><Day>17</Day></RegistrationDate><Received><Year>1992</Year><Month>4</Month><Day>7</Day></Received><Accepted><Year>1992</Year><Month>7</Month><Day>14</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName><CopyrightYear>1992</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>11103</JournalID><VolumeIDStart>20</VolumeIDStart><VolumeIDEnd>20</VolumeIDEnd><IssueIDStart>5</IssueIDStart><IssueIDEnd>5</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Pascal</GivenName><FamilyName>Genschik</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yves</GivenName><FamilyName>Parmentier</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Andrée</GivenName><FamilyName>Durr</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Jacqueline</GivenName><FamilyName>Marbach</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Marie-Claire</GivenName><FamilyName>Criqui</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Elisabeth</GivenName><FamilyName>Jamet</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Jacqueline</GivenName><FamilyName>Fleck</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgName>Institut de Biologie Moléculaire des Plantes du C.N.R.S.</OrgName><OrgAddress><Street>12 rue du Général Zimmer</Street><Postcode>67084</Postcode><City>Strasbourg Cédex</City><Country>France</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of <Emphasis Type="Italic">Nicotiana sylvestris</Emphasis>. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5- mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl<Subscript>2</Subscript> treatment, a viral infection giving rise to a hypersensitive reaction, and an <Emphasis Type="Italic">Agrobacterium tumefaciens</Emphasis> infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.</Para></Abstract><KeywordGroup Language="En"><Heading>Key words</Heading><Keyword>Cell division</Keyword><Keyword>gene expression</Keyword><Keyword><Emphasis Type="Italic">Nicotiana sylvestris</Emphasis></Keyword><Keyword>protoplast</Keyword><Keyword>stress</Keyword><Keyword>ubiquitin</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses</title></titleInfo><name type="personal"><namePart type="given">Pascal</namePart><namePart type="family">Genschik</namePart><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yves</namePart><namePart type="family">Parmentier</namePart><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Andrée</namePart><namePart type="family">Durr</namePart><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jacqueline</namePart><namePart type="family">Marbach</namePart><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marie-Claire</namePart><namePart type="family">Criqui</namePart><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Elisabeth</namePart><namePart type="family">Jamet</namePart><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jacqueline</namePart><namePart type="family">Fleck</namePart><affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12 rue du Général Zimmer, 67084, Strasbourg Cédex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Kluwer Academic Publishers</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateCreated encoding="w3cdtf">1992-04-07</dateCreated><dateIssued encoding="w3cdtf">1992-12-01</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5- mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.</abstract><note>Research Articles</note><subject lang="en"><genre>Key words</genre><topic>Cell division</topic><topic>gene expression</topic><topic>Nicotiana sylvestris</topic><topic>protoplast</topic><topic>stress</topic><topic>ubiquitin</topic></subject><relatedItem type="host"><titleInfo><title>Plant Molecular Biology</title><subTitle>An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</subTitle></titleInfo><titleInfo type="abbreviated"><title>Plant Mol Biol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1992-12-01</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Biochemistry, general</topic><topic>Plant Sciences</topic><topic>Plant Pathology</topic></subject><identifier type="ISSN">0167-4412</identifier><identifier type="eISSN">1573-5028</identifier><identifier type="JournalID">11103</identifier><identifier type="IssueArticleCount">24</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>1992</date><detail type="volume"><number>20</number><caption>vol.</caption></detail><detail type="issue"><number>5</number><caption>no.</caption></detail><extent unit="pages"><start>897</start><end>910</end></extent></part><recordInfo><recordOrigin>Kluwer Academic Publishers, 1992</recordOrigin></recordInfo></relatedItem><identifier type="istex">33D1C251C3F6EC1B5503A2CEE68D2470C907AB9F</identifier><identifier type="ark">ark:/67375/1BB-K0DM107N-M</identifier><identifier type="DOI">10.1007/BF00027161</identifier><identifier type="ArticleID">BF00027161</identifier><identifier type="ArticleID">Art15</identifier><accessCondition type="use and reproduction" contentType="copyright">Kluwer Academic Publishers, 1992</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Kluwer Academic Publishers, 1992</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-K0DM107N-M/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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